CRISPR/Cas9 FAQs Answered!
Type
Blog Post
Published
March 13, 2014, 4:08 p.m.
...never used the Ex Taq. In our hands, Herculase II Fusion polymerase or Kapa Hifi Polymerase work very well... (meaning the mature processed form of crRNA is fused chimerically to a mature tracrRNA), the spacer used...