We narrowed to 291 results for: fus

1. CRISPR Cheat Sheet

   Type

   Blog Post

   Published

   Jan. 27, 2021, 2 p.m.

   ...nucleases that cut or modify RNA as opposed to DNA. Fusing the nuclease to an adenosine deaminase can convert...

2. The Developmental Studies Hybridoma Bank: Over 25 Years of Antibody Sharing

   Type

   Blog Post

   Published

   March 10, 2015, 2:25 p.m.

   ...monoclonal antibody cell line, myeloma cells are fused with activated B cells from the spleen of mice injected...

3. Measuring Kinase Activity at the Single-Cell Level with Kinase Translocation Reporters (KTRs)

   Type

   Blog Post

   Published

   July 26, 2018, 12:46 p.m.

   ...substrate for a kinase of interest which is further fused to a bipartite nuclear localization signal (bNLS...

4. Editor's Choice, August 2016

   Type

   Blog Post

   Published

   Sept. 2, 2016, 1:32 p.m.

   ...catalytically inactive CRISPR endonuclease (dCas9) fused to a deaminase to make the transitions at target...

5. Scientist Networking: What is an Informational Interview?

   Type

   Blog Post

   Published

   July 1, 2014, 3:42 p.m.

   ...might say yes and they might say no but do not confuse networking for a job connection with ongoing relationship...

6. Seeing Red: Simple GFP Photoconversion

   Type

   Blog Post

   Published

   Aug. 4, 2015, 2:30 p.m.

   ... saturates around 30 uM. For low-abundance GFP fusions, a photoconversion signal may not be observed. ...

7. IDP and your PI: A Roadmap for Career Planning and Personal Development

   Type

   Blog Post

   Published

   July 21, 2015, 2:30 p.m.

   ...complete their work rather than overwhelmed or confused about what’s coming next. Goal setting and career...

8. What's New in CRISPR - March 2020

   Type

   Blog Post

   Published

   March 10, 2020, 1:15 p.m.

   ...Casilio-ME1. Image from Taghbalout et al., 2019. Fusing epigenetic modifiers such as TET1 with inactive...

9. Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot

   Type

   Blog Post

   Published

   Dec. 5, 2019, 1:49 p.m.

   ...hard to find. This made the page look not only confusing, but also made it hard for users to get where ...

10. Mapping the 4D nucleome with CRISPR/Cas9

    Type

    Blog Post

    Published

    Aug. 11, 2015, 2:30 p.m.

    ...orthologs NmCas9 and St1Cas9. Each ortholog was fused to a different fluorescent protein to create three...

11. Cre-ating New Methods for Site-specific Recombination in Drosophila

    Type

    Blog Post

    Published

    May 12, 2015, 1:32 p.m.

    .... A Cre-estrogen receptor ligand binding domain-fusion can prevent this toxicity, but with the caveat ...

12. SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture

    Type

    Blog Post

    Published

    Aug. 13, 2014, 3:49 p.m.

    ...amino acids, but SpyCatcher is a protein. You can fuse SpyCatcher onto things, but even better if one could...

13. Build Your CRISPR Vocabulary

    Type

    Blog Post

    Published

    July 2, 2024, 1:15 p.m.

    ... it have a lot of parts! If you’re still a bit confused by CRISPR acronyms, this post is for you. We’ll...

14. Year of the Camelid: Antibody Style

    Type

    Blog Post

    Published

    May 9, 2024, 1:15 p.m.

    ... which are single-domain antibodies genetically fused to fluorescent proteins and used to visualize target...

15. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type

    Blog Post

    Published

    June 11, 2020, 1:34 p.m.

    ...chemogenetics relies on chemical activation and the diffusion of drugs throughout the body, the onset of receptor...

16. Understanding GPCR Signaling Using TRUPATH

    Type

    Blog Post

    Published

    Dec. 14, 2020, 2:15 p.m.

    ...constructs. For the Gγ constructs, the GFP2 tag is fused to the N-terminus of the protein. For the Gα constructs...

17. New Viral Vectors - Winter 2025

    Type

    Blog Post

    Published

    Jan. 28, 2025, noon

    ...Scanziani New viral prep pAAV_hSyn1-SIO-stGtACR2-FusionRed AAV5 Optogenetics Ofer Yizhar New serotype pAAV-CaMKIIa-ChRmine-oScarlet-KV...

18. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type

    Blog Post

    Published

    Aug. 31, 2023, 1 p.m.

    ... (ChR2), a blue light sensitive cation channel, fused to mCherry to the brain tissue. They performed optical...

19. Antibodies 101: Epitope Tags

    Type

    Blog Post

    Published

    May 11, 2023, 1:15 p.m.

    ... References Terpe, K., Overview of tag protein fusions: from molecular and biochemical fundamentals to...

20. Intro to Our Summer SciComm Series!

    Type

    Blog Post

    Published

    June 9, 2022, 1:15 p.m.

    ....” You can see how you could end up in a very confusing situation rather quickly!  The question   The...