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We narrowed to 295 results for: fus

Showing: 181 - 200 of 295 results
  1. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ...never used the Ex Taq. In our hands, Herculase II Fusion polymerase or Kapa Hifi Polymerase work very well... (meaning the mature processed form of crRNA is fused chimerically to a mature tracrRNA), the spacer used...
  2. CRISPR Cheat Sheet

    Type
    Blog Post
    Published
    Jan. 27, 2021, 2 p.m.
    ...nucleases that cut or modify RNA as opposed to DNA. Fusing the nuclease to an adenosine deaminase can convert...
  3. Purple Plants that Report Pollutants

    Type
    Blog Post
    Published
    March 3, 2026, 2:14 p.m.
    ...suited for detection of chemicals that readily diffuse through plant tissue. Those might be the most important...
  4. Editor's Choice, August 2016

    Type
    Blog Post
    Published
    Sept. 2, 2016, 1:32 p.m.
    ...catalytically inactive CRISPR endonuclease (dCas9) fused to a deaminase to make the transitions at target...
  5. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    Published
    Aug. 4, 2015, 2:30 p.m.
    ... saturates around 30 uM. For low-abundance GFP fusions, a photoconversion signal may not be observed. ...
  6. What's New in CRISPR - March 2020

    Type
    Blog Post
    Published
    March 10, 2020, 1:15 p.m.
    ...Casilio-ME1. Image from Taghbalout et al., 2019. Fusing epigenetic modifiers such as TET1 with inactive...
  7. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    Published
    Aug. 11, 2015, 2:30 p.m.
    ...orthologs NmCas9 and St1Cas9. Each ortholog was fused to a different fluorescent protein to create three...
  8. Build Your CRISPR Vocabulary

    Type
    Blog Post
    Published
    July 2, 2024, 1:15 p.m.
    ... it have a lot of parts! If you’re still a bit confused by CRISPR acronyms, this post is for you. We’ll...
  9. Year of the Camelid: Antibody Style

    Type
    Blog Post
    Published
    May 9, 2024, 1:15 p.m.
    ... which are single-domain antibodies genetically fused to fluorescent proteins and used to visualize target...
  10. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    Published
    Dec. 14, 2020, 2:15 p.m.
    ...constructs. For the Gγ constructs, the GFP2 tag is fused to the N-terminus of the protein. For the Gα constructs...
Showing: 181 - 200 of 295 results