We narrowed to 515 results for: gats

...closer look at this new functionality and ways to navigate it, we’ll search for the calcium biosensor, GCaMP... in Parkinson’s disease. Knowing the primary investigator where the plasmid was created can narrow your... keeps more than half of these. Instead we can navigate first to the CRISPR Resources page: The page ...

...of Research, our volunteers have been helping to gather information for our FLSA and postdocs resource ...points on this salary range. We have been able to gather data on changes to salary for some of the institutions...advocating for and with junior scientists, to help them navigate the scientific enterprise and promote their voices...

...preparation after negative staining. Empty vector particles can be identified after negative staining and ...proteins) as opposed to purified DNA. This may be mitigated by using purified viral DNA as the template instead...

...Many CRISPR screens use non-targeting guides as negative controls. Non-targeting guides do not target any...sequence in the genome, and are commonly used as negative controls for genome-wide CRISPR screens; and 3... Less significant p-values lead to more false-negatives results (i.e. failing to identify a gene that’...

...recently was invited to attend the first Principal Investigator meeting of the NSF EDGE (Enabling Discovery ...experimental species. Discovering ways to culture, propagate, transform, store, and share all of these new ...cephalopods die after they lay their 1 clutch of eggs? Propagation is thus quite difficult. CRISPR genome editing...

...latent HIV reservoirs, chronic inflammation, and negative metabolic effects of ART. Why can’t the body mount...effects were detected. Kaminski et al. observed no negative effects on cell viability, cell cycle progression...would make it easier for an “escape” virus to propagate and infect adjacent cells. It will be important...

...2013). Since then, Spinach2 has been used to investigate intracellular viral DNA dynamics in neuronal ...developed into a split aptamer that can be used to investigate RNA assembly. The split aptamer is composed of... blog Learn more about aptamer fluorophores Investigate other fluorescent biosensors Find other uses ...

...confidential (see image below). Slide of information gathered by Postdoctoral Associations around Boston - From...who can provide additional advice to trainees navigating the waters of career development. Publishing ...individual is then judged objectively and possibly negatively by a third party, it can come as a shock to the...

...photoinstability, as well as the tendency of fluorophores to aggregate in the neurons’ somata. Second, the system did...to find those with ideal characteristics (low aggregation, high photostability, and high stability after...immunostaining. This feature facilitates screening of Cre-negative Brainbow mice to determine the number and type...

...dynamic labeling power of miniTurboID, a proximity ligation tool (Cenik et al., 2024). While dCas9 has been...courtesy of Brianna Monroe, MS. Proximity ligation tools like TurboID and its mini counterpart label... location. TurboID improved on other proximity ligation tools with better kinetics and less background...

...characterization, these proteins could function as surrogate antibodies for identifying and characterizing ...with T4 DNA polymerase (to create blunt ends) and ligate into a bluntly digested vector designed for protein...presence of certain modifications to DNA bases.  Ligate and transform your resultant plasmid libraries ...

...extract the linearized band, ligate the plasmid, and re-transform the ligated plasmid to recover the monomeric...version of the plasmid. Linearize, gel extract, and ligate the plasmid If you are having difficulty isolating...

...yet powerful way to tag cellular proteins and investigate protein localization, interaction, and expression...the early reversible PA-FPs also display this “negative” photoswitching. In 2008, scientists used mutagenesis...function and localization, or leading to protein aggregation. Another important parameter to consider is the...

...achieves ultra-low false-positive (0–9%) and false-negative (0–19%) rates. In clinically relevant HSPC models...Shivalila described Wave Life Science’s GalNAc-conjugated AIMer platform, which engages endogenous ADAR...., Reyon, D., & Duffield, J. S. (2025). 3′-end ligation sequencing is a sensitive method to detect DNA...

...page. The data produced is available on the F1000 gateway and Zenodo community.  Knockdown approach For... have the same clone available with different conjugates. Summary Flow cytometry is a powerful tool to...resources from the Addgene blog Introduction to Gating in Flow Cytometry Antibodies 101: Flow Cytometry...

...covered in this segment Hot Plasmid 1: The Tol2 Gateway-Compatible Toolbox for quickly generating zebrafish... diseases. Listen to Our Segement on the Tol 2 Gateway-Compatible Toolbox  Hot Plasmid 2: AAV Vectors ...

...the blog’s newly updated navigation menu and search tool to help you navigate the scavenger hunt. Good...

... Singapore. Chapter 4: Life as a new primary investigator. Coming up with new project ideas, applying ... Chew! Wei Leong Chew is a Junior Principal Investigator at the Genome Institute of Singapore. His lab...

...protocols.  These feature step-by-step instructions to navigate various lab processes.  Our most recent protocol...videos feature screen-capture footage to help you navigate Addgene’s vast online resources. Our most popular...

...breakthrough research. Insights about how to navigate through all stages of your career, whether you...lab manager, research technician, or Principal Investigator. How-to guides for troubleshooting your experiments...