We narrowed to 513 results for: gats

...vitro, before transforming it into E. coli for propagation. However, while not widely known, plasmid assembly...Laboratory of Molecular Biology. He is currently investigating the molecular biology of synaptic transmission...and plasticity.     Javier García-Nafría is an investigator scientist at the MRC Laboratory of Molecular...

...Many CRISPR screens use non-targeting guides as negative controls. Non-targeting guides do not target any...sequence in the genome, and are commonly used as negative controls for genome-wide CRISPR screens; and 3... Less significant p-values lead to more false-negatives results (i.e. failing to identify a gene that’...

...preparation after negative staining. Empty vector particles can be identified after negative staining and ...proteins) as opposed to purified DNA. This may be mitigated by using purified viral DNA as the template instead...

...recently was invited to attend the first Principal Investigator meeting of the NSF EDGE (Enabling Discovery ...experimental species. Discovering ways to culture, propagate, transform, store, and share all of these new ...cephalopods die after they lay their 1 clutch of eggs? Propagation is thus quite difficult. CRISPR genome editing...

...of Research, our volunteers have been helping to gather information for our FLSA and postdocs resource ...points on this salary range. We have been able to gather data on changes to salary for some of the institutions...advocating for and with junior scientists, to help them navigate the scientific enterprise and promote their voices...

...2013). Since then, Spinach2 has been used to investigate intracellular viral DNA dynamics in neuronal ...developed into a split aptamer that can be used to investigate RNA assembly. The split aptamer is composed of... blog Learn more about aptamer fluorophores Investigate other fluorescent biosensors Find other uses ...

...latent HIV reservoirs, chronic inflammation, and negative metabolic effects of ART. Why can’t the body mount...effects were detected. Kaminski et al. observed no negative effects on cell viability, cell cycle progression...would make it easier for an “escape” virus to propagate and infect adjacent cells. It will be important...

...confidential (see image below). Slide of information gathered by Postdoctoral Associations around Boston - From...who can provide additional advice to trainees navigating the waters of career development. Publishing ...individual is then judged objectively and possibly negatively by a third party, it can come as a shock to the...

...photoinstability, as well as the tendency of fluorophores to aggregate in the neurons’ somata. Second, the system did...to find those with ideal characteristics (low aggregation, high photostability, and high stability after...immunostaining. This feature facilitates screening of Cre-negative Brainbow mice to determine the number and type...

...extract the linearized band, ligate the plasmid, and re-transform the ligated plasmid to recover the monomeric...version of the plasmid. Linearize, gel extract, and ligate the plasmid If you are having difficulty isolating...

...dynamic labeling power of miniTurboID, a proximity ligation tool (Cenik et al., 2024). While dCas9 has been...courtesy of Brianna Monroe, MS. Proximity ligation tools like TurboID and its mini counterpart label... location. TurboID improved on other proximity ligation tools with better kinetics and less background...

...characterization, these proteins could function as surrogate antibodies for identifying and characterizing ...with T4 DNA polymerase (to create blunt ends) and ligate into a bluntly digested vector designed for protein...presence of certain modifications to DNA bases.  Ligate and transform your resultant plasmid libraries ...

...yet powerful way to tag cellular proteins and investigate protein localization, interaction, and expression...the early reversible PA-FPs also display this “negative” photoswitching. In 2008, scientists used mutagenesis...function and localization, or leading to protein aggregation. Another important parameter to consider is the...

...page. The data produced is available on the F1000 gateway and Zenodo community.  Knockdown approach For... have the same clone available with different conjugates. Summary Flow cytometry is a powerful tool to...resources from the Addgene blog Introduction to Gating in Flow Cytometry Antibodies 101: Flow Cytometry...

...covered in this segment Hot Plasmid 1: The Tol2 Gateway-Compatible Toolbox for quickly generating zebrafish... diseases. Listen to Our Segement on the Tol 2 Gateway-Compatible Toolbox  Hot Plasmid 2: AAV Vectors ...

...the blog’s newly updated navigation menu and search tool to help you navigate the scavenger hunt. Good...

... Singapore. Chapter 4: Life as a new primary investigator. Coming up with new project ideas, applying ... Chew! Wei Leong Chew is a Junior Principal Investigator at the Genome Institute of Singapore. His lab...

...protocols.  These feature step-by-step instructions to navigate various lab processes.  Our most recent protocol...videos feature screen-capture footage to help you navigate Addgene’s vast online resources. Our most popular...

...breakthrough research. Insights about how to navigate through all stages of your career, whether you...lab manager, research technician, or Principal Investigator. How-to guides for troubleshooting your experiments...

...electricity aggregation program” for all local households and businesses. Electricity aggregation allows a...