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We narrowed to 415 results for: genome

Showing: 181 - 200 of 415 results

  1. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    Published
    Oct. 19, 2023, 1:15 p.m.
    ...an AAV plasmid an AAV plasmid.  Within the AAV genome, ITRs serve as the origin of replication and are... that flank the ends of the single stranded DNA genome of AAV in the native virus. Since the WT sequence...nicking. Since Rep proteins are important for viral genome replication, these mutations can affect transduction...Inverted Terminal Repeats (ITRs) on the Productivity, Genome Packaging, and Potency of AAV Vectors. Microorganisms...

  2. X-CHIME: Context Dependent Germline Knockout in Immune Cells

    Type
    Blog Post
    Published
    Jan. 16, 2024, 6:13 p.m.
    ...CRISPR101: Validating Your Genome Edit How to Design Your gRNA for CRISPR Genome Editing CRISPR Cheat Sheat...from Addgene.org CRISPR Guide CRISPR: Protocol for Genome Deletions in Mammalian Cell Lines    ...

  3. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    Published
    Aug. 11, 2015, 2:30 p.m.
    ...uses fluorescently labeled Cas9 to label multiple genomic loci in live cells. While other systems can be ...is a great step forward in live cell imaging of genomic loci. Ma et al. anticipate that the technique will...this publication at Addgene. Dynamic imaging of genomic loci in living human cells by an optimized CRISPR...

  4. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    Published
    Jan. 16, 2025, 2:15 p.m.
    ...modifying capsid and genomic structures. For example, rAAV pseudotypes mix the genome of one AAV serotype... AAV vectors have both distinctive capsids and genomic structures. The viral capsid is the key that lets...separated by a slash: the first number refers to the genome, and the second number to the capsid. Most vectors... provided by Addgene are pseudotypes using the genomic structure of AAV2, so they are formally called ...best studied and most commonly used serotype. Its genome is the most common choice for AAV pseudotyping....

  5. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    Published
    March 21, 2017, 2:30 p.m.
    ...broader range of cells and their integration into the genome is more efficient. The first retroviral vectors...three plasmids encoding 1) the lentiviral vector genome which was composed of the wild-type 5’ and 3’ LTRs...desired gene (transfer vector plasmid), 2) the HIV-1 genome containing all viral genes with the exception of...LTR so you don’t need Tat to transcribe the viral genome in producing cells. Currently, the third-generation...still infect the researcher and insert into her/his genome - this is a biosafety risk Infects dividing and...proteins generated Stable integration into host genome and stable expression of the transgene   Transfer...

  6. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    Published
    Aug. 29, 2024, 1:15 p.m.
    ...stable fly cell lines; attL and attR sequences for genome integration by phiC31) Drosophilia Plasmid...fly cell lines; Hygromycin resistance gene; for genome integration by spontaneous insertion)   Drosophilia...sequences; white + selectable marker; attB site for genome integration by phiC31   The Fragmid website...assessment of emerging CRISPR technologies. Cell Genomics, 4(3), 100519. https://doi.org/10.1016/j.xgen.2024.100519...

  7. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type
    Blog Post
    Published
    Dec. 14, 2021, 2:15 p.m.
    ...loci – in neighboring chromatin or throughout the genome – could be affected, leading to unpredicted gene...targeting, including modifying other sites in the genome and directly interacting with transcription machinery...DNMT1 from that site. So, when DNMT1 methylates the genome during DNA replication, it doesn’t methylate sites...Galonska C, Charlton J, Mattei AL et al (2018). Genome-wide tracking of dCas9-methyltransferase footprints...Resources on the Addgene blog CRISPR101: Editing the Epigenome  Mapping the 4D Nucleome with CRISPR/Cas9 Resources...

  8. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ...Cas9 technology has been a breakthrough for the genome-editing field and the greater scientific community...several strains of E. coli and lethally chop their genomes into many, many pieces. Locus Biosciences is currently...employs Cas9 to cut at two sites within the viral genome to excise a large portion of it. This ongoing trial... Zarghamian P, Klermund J, Cathomen T. Clinical genome editing to treat sickle cell disease-A brief update...

  9. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    Published
    Sept. 24, 2015, 2:30 p.m.
    .... CRISPR technology has been widely adopted for genome editing purposes because it's cheaper, faster, ...be used in vivo. A common choice for conducting genome-wide screens using CRISPR. AAV transduction Only...evaluate lentiviral component delivery as well as genome repair by non-homologous end joining (NHEJ) or ...component delivery and sgRNA efficacy by cloning in genome target sequence into EGFP reporter. Target DNA ...

  10. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ...2022b). Genome editing technology and applications with the type I CRISPR system. Gene and Genome Editing...CRISPR, by any other name would…probably still edit genomes. Understanding the different types of CRISPR systems...might imagine, this is handy for creating large genomic deletions. Type I systems are also employed as ...Marraffini, L. A., & Zhang, F. (2013). Multiplex genome engineering using CRISPR/CAS systems. Science, ...inactivation mechanisms in type I-C CRISPR-Cas3 for genome-editing applications. Molecular Cell, 84(3), 463... expanding biotechnology toolbox for and beyond genome editing. Cell & Bioscience, 8(1). https://doi.org...

  11. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    Published
    Aug. 11, 2020, 1:15 p.m.
    ...and the transfer plasmid which contains the viral genome. In today’s blog post, we’ll focus on the AAV ...transgene. The WPRE is located at the end of the AAV genome, just upstream of the final ITR.  polyA  The polyadenylation...longevity. The polyA is located at the end of the AAV genome, just upstream of the final ITR and downstream ...

  12. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    Published
    April 2, 2020, 6:17 p.m.
    ...specific, controlled changes to an organism’s genome (genome engineering) Produce synthetic viruses that...plasmids, reporter plasmids, viral plasmids, and genome engineering plasmids. Some of the many things that...

  13. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    Published
    Feb. 13, 2018, 2:51 p.m.
    ... go-to method for mapping where a protein binds genome-wide, and has been widely applied in many model...proteins may appear to be binding at particular genomic sites when in fact they are just non-specific or...protocols Browse CRISPR plasmids for isolating a genomic locus Browse FRET plasmids for studying protein...

  14. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    Published
    July 27, 2021, 1:15 p.m.
    ...ready-to-use viral preps New toolkit for CRISPR-based genome editing in organoids By Ashley Waldron Organoids...at temperatures up to 43 °C and can be used for genome editing in moderate thermophiles.  These evolved...activity on NGG PAMs relative to wild type. A new genome-wide CRISPR knockout library from Xiaole Shirley...

  15. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

    Type
    Blog Post
    Published
    Nov. 12, 2019, 2 p.m.
    ...profiling samples, and years later, several whole genome sequencing projects were launched in the Quake ... Schmidt not only produced partially assembled genomes, but also led to the discovery of IgnaviCas9, a...CRISPR array in the resulting Ignavibacteriae phylum genome. IgnaviCas9 is a hyperthermostable Cas9 CRISPR ...Spring in Lower Geyser Basin. They used a mini-metagenomics method using microfluidic parallelization to...

  16. CRISPR 101: Drug discovery and CRISPR

    Type
    Blog Post
    Published
    May 4, 2023, 1 p.m.
    ...model generation CRISPR has made the generation of genome edits accessible, fast, and easy. In cases where...Line Using CRISPR-Cas9 to Edit Disease Out of the genome Tips for a first time CRISPR User (by a first ...and Resources CRISPR History and Development for Genome Engineering    ...

  17. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type
    Blog Post
    Published
    Sept. 15, 2015, 2:30 p.m.
    ...Every year, the Rare Genomics Institute sponsors a global competition for researchers working on rare...disease.” He says that the award from the Rare Genomics Institute and Addgene will make a substantial ...Additional Resources Find out more about the Rare Genomics Institute and the BeHeard Award!    ...

  18. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ... show the potential of base editing for precise genome modification. Plasmid ID Plasmid Name Cas9 Variant...library. Although B. subtilis has a fully sequenced genome, many applications in biotech, and is considered... length is important for cellular function and genomic stability, as telomere shortening leads to premature...regulating telomere length with TZAP Multiplexible plant genome engineering toolkit The Voytas Lab at the University...that can be used for either TALEN or CRISPR -based genome engineering across a variety of plant species. ...identified using a bioinformatics approach examining genomes possessing CRISPR systems with apparent self-targeting...inhibitors may provide a layer of regulation to genome engineering that enables new applications, such...

  19. Five Popular Model Organisms

    Type
    Blog Post
    Published
    April 11, 2019, 12:39 p.m.
    ...The zebrafish reference genome sequence and its relationship to the human genome." Nature496.7446 (2013...every 2 hours. Yeast are also the first eukaryotic genome to be entirely sequenced and is very amenable to...one-celled embryos with DNA or RNA to edit their genomes or to create transgenic animals. Zebrafish are ...Similarly to other model organisms, the C.elegans genome is also completely sequenced and over 60% of human...

  20. Addgene Protocols Go Viral: AAV Purification and Titration

    Type
    Blog Post
    Published
    June 13, 2019, 12:19 p.m.
    ...described in this blog post, the team developed a viral genome sequencing strategy to confirm the identity and... successfully set up your qPCR to determine the genome copies in a given AAV preparation. For detailed...
Showing: 181 - 200 of 415 results