We narrowed to 256 results for: idua

1. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

   Type

   Blog Post

   Published

   May 1, 2018, 1:57 p.m.

   ... particular protein  complex as opposed to an individual protein. The Bethune Lab has overcome this limitation...

2. Lab Automation at Addgene

   Type

   Blog Post

   Published

   March 22, 2018, 1:28 p.m.

   ...same time as opposed to preparing each sample individually. Enter the BIOROBOT! This robot is called the...

3. Adeno Associated Virus (AAV) for Cell and Gene Therapy

   Type

   Blog Post

   Published

   Nov. 7, 2017, 1:59 p.m.

   ...their efficacy:  Pre-existing immunity: Many individuals already have wt AAV in their bodies and may therefore...

4. Hot Plasmids and Viral Preps - January 2021

   Type

   Blog Post

   Published

   Jan. 5, 2021, 2:15 p.m.

   ...Tagging with Light (SPOTlight) as a way to isolate individual cells with unique spatiotemporal phenotypes from...

5. How to Prepare for an Industry Interview

   Type

   Blog Post

   Published

   July 9, 2020, 1:15 p.m.

   ...however, were more structured. I met with several individual and/or small groups, usually starting with the...

6. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

   Type

   Blog Post

   Published

   June 26, 2018, 1:33 p.m.

   ...could improve health outcomes for millions of individuals worldwide.Arg is unique among amino acids in ...

7. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

   Type

   Blog Post

   Published

   Aug. 20, 2019, 12:31 p.m.

   ...Animal Strain, age, sex, housing conditions (individually versus in groups), health status, diet, cage...

8. Open Resources and Plasmid Tools For Studying C. elegans

   Type

   Blog Post

   Published

   July 18, 2019, 12:55 p.m.

   ...embryo when all essential embryonic genes are individually depleted via RNA interference (RNAi). Scientists...

9. Illuminating Epigenetics with A FRET Based Biosensor

   Type

   Blog Post

   Published

   Nov. 19, 2015, 3:30 p.m.

   ...maternal copy of the gene is expressed in normal individuals (in a parent-of-origin-specific manner), but ...

10. The Materials Science of Optogenetics Experiments

    Type

    Blog Post

    Published

    Sept. 17, 2015, 2:30 p.m.

    ...contexts/locations in the test apparatus and thus individual laser stimulation is essential. In terms of the...

11. Viral Vectors 101: Producing Your rAAV

    Type

    Blog Post

    Published

    July 16, 2024, 1:15 p.m.

    ...with DNase before undergoing titering, to remove residual plasmid DNA carried over from the production process...

12. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type

    Blog Post

    Published

    Oct. 12, 2017, 2:26 p.m.

    ...screen, it is expensive and time consuming to individually generate gRNAs and homology arm repair templates...

13. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type

    Blog Post

    Published

    April 4, 2019, 12:53 p.m.

    ...and colleagues used Mobile-CRISPRi to knockdown individual genes in several bacteria associated with human...

14. Academic vs. Industry Postdocs

    Type

    Blog Post

    Published

    Nov. 13, 2018, 1:22 p.m.

    ...team: Industry is all about teamwork. A single individual could not bring a drug to the market, so people...

15. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type

    Blog Post

    Published

    Oct. 23, 2018, 11:49 a.m.

    ...samples are first DNaseI digested to remove any residual AAV plasmid that was carried over from the AAV...

16. Transferable Skills: Negotiation

    Type

    Blog Post

    Published

    March 3, 2022, 2 p.m.

    ...examples depend on many specific details about the individuals, each example provides a scenario where both ...

17. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type

    Blog Post

    Published

    Sept. 12, 2017, 1:44 p.m.

    ...step process using Geneious software: First, the individual sequencing reads (~150 bp each) are aligned to...

18. Plasmids 101: Optimizing Plasmid Yields

    Type

    Blog Post

    Published

    May 26, 2016, 2:30 p.m.

    ...determined for each plasmid/strain combination individually, based on determining the cell density or by...

19. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type

    Blog Post

    Published

    May 22, 2015, 4:58 p.m.

    ...can restore the wild-type sequence (save for the residual sgRNAs and possibly cas9 gene). An immunizing ...

20. Plasmids 101: Multicistronic Vectors

    Type

    Blog Post

    Published

    Sept. 9, 2014, 8:20 p.m.

    ...-expression by using a plasmid with multiple, individual expression cassettes, having the genes expressed...