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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 406 results for: mag

Showing: 181 - 200 of 406 results

  2. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ... fluorescent indicators for organellar calcium imaging. Biophysical journal. 2016 Sep 20;111(6):1119-31...2014 Nov 18;7:88. Tian L, Hires SA, Looger LL. Imaging neuronal activity with genetically encoded calcium...

  3. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ...Reports 9: https://doi.org/10.1038/s41598-019-41088-2 Magnus C, Lee P, Bonaventura J, Zemla R, Gomez J, Ramirez...applications. Science 6436: eaav5282. https://science.sciencemag.org/content/364/6436/eaav5282.long Redfern ...

  6. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    Published
    April 6, 2017, 1:02 p.m.
    ...Cell Collection at Coriell. More information and images generated from these cell lines can be found in...fluorescent proteins as described here enables live cell imaging to study cellular structures and processes and ...

  7. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    Published
    Sept. 27, 2017, 1:23 p.m.
    ...offer guided tours to show people where the lab magic is happening. 3) Volunteer – Step into science education...learning on the job. Try to get an internship at a magazine or newspaper (here’s a partial list). A great ...

  8. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...which will bind and light up a tagged protein for imaging. Given its popularity and widespread usage, a proteolysis-targeting... avoid AID tags. If your project also involves imaging at some stage, then choosing a HaloTag could pay...

  9. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    Published
    April 26, 2022, 1:15 p.m.
    ...fluorescent signaling in the presence of dsDNA. Image courtesy of: Ygonaar    Taqman is more sensitive...thousands of PCR reactions using Taqman chemistry (see image, left, of droplet immersion technique, courtesy ...

  10. Transferable Skills: Negotiation

    Type
    Blog Post
    Published
    March 3, 2022, 2 p.m.
    ...confocal microscope. You’re trying to get better images for a paper and she’s trying to get some data for...manuscript is more important to you than collecting your images this week. Even though three examples depend on...

  11. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    Published
    April 4, 2019, 12:53 p.m.
    ... Figure 1: Mobile-CRISPRi's modular components. Image from Peters et al., 2019 with permission.   ...recipient bacteria with an integrated copy of CRISPRi. Image from Peters et al., 2019 with permission.    ...

  13. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    Published
    May 22, 2015, 4:58 p.m.
    ... extend far beyond the laboratory and seriously damage public trust in scientists, experiments involving...technologies with care and humility. Nothing would damage that trust more than an accidental gene drive release...

  14. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...Fluorescent proteins and their applications in imaging living cells and tissues”  Physiol. Rev. 90:1103...et. al. (2011) “Modern fluorescent proteins and imaging technologies to study gene expression, nuclear ...

  15. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ...   Figure 2: Structure of a retrovirus. Image re-used from Dong & Kantor, 2021 under CC-BY license...   Figure 3: The life cycle of a retrovirus. Image courtesy of Jeremy Luban, MD. Figure 3 depicts...

  16. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ...fluorescent label, which can reduce the potential image resolution of super-resolution fluorescence microscopy...Nanobodies: site-specific labeling for super-resolution imaging, rapid epitope-mapping and native protein complex...

  17. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    Published
    May 20, 2025, 1:15 p.m.
    ...I fell in love with biology because of an image that was honestly quite boring. My Bio 101 professor ...sorting or to visually select cells in a microscopy image for individual analysis. A shuttle vector designed...

  18. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    Published
    Oct. 13, 2022, 1:45 p.m.
    ...knock in, base edit, activate, repress, and even image your favorite gene. With so many Cas9 reagents available...target strand based on the sequence of the pegRNA. Image from David Liu.   Activation and repression...

  19. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    Published
    April 13, 2021, 1:15 p.m.
    .... Steps 1-1 and 1-2 can be done simultaneously. Image from Li et al., 2021.   The updates involved...gene dosage on N-acetylglucosamine concentration. Image from the Yang lab.   In conclusion, the MUCICAT...

  20. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    Published
    Nov. 19, 2020, 2:15 p.m.
    ...-inducible Cre not only halted the neurological damage but completely reversed it, regardless of age or...corrected by CRISPR/Cas9 in wild-type human iPSCs. Image modified from Le et al., 2019. Gene editing ...
Showing: 181 - 200 of 406 results