Skip to main content

We narrowed to 414 results for: mag

Showing: 181 - 200 of 414 results
  1. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    Published
    May 26, 2020, 1:15 p.m.
    ... Figure 1: The SRIRACCHA reporter construct. Image from Wen et al., 2017. Once you’ve introduced...fluorescence.  Figure 2: The GEmCherry reporter. Image from Højland Knudsen et al., 2018. So how does...
  2. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    Published
    June 29, 2021, 1:15 p.m.
    ... hybridomas for monoclonal antibody production. Image created with BioRender.com.   The antibody-producing...different antibodies produced by the hybridoma. Image from Bradbury et al., 2018.   Another barrier...
  3. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    Published
    June 12, 2018, 11:40 a.m.
    ...chromatin recognition of transcription-associated DNA damage that promotes homologous recombination. Gong F,...small, sensitive immunohistochemical reagents. Yamagata M, Sanes JR Proc Natl Acad Sci U S A. 2018 Feb...
  4. All in a Twist: dsRNA

    Type
    Blog Post
    Published
    Feb. 2, 2023, 2:15 p.m.
    ... molecules are clearly associated with cellular damage and dysfunction, it is unclear what the precise... that is now vulnerable to various forms of DNA damage, and these structures can in turn lead to genome...
  5. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ... space that would size-exclude an antibody. In imaging applications, smaller affinity reagents means higher...often used for clinical applications, including imaging.  Diabodies are ~60 kDa proteins formed by two ...
  6. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    Published
    Dec. 8, 2016, 3:30 p.m.
    ...succumb to the toxicity of CcdB.   Figure 1: Image from Bernard, P., et al. showing the pKIL 18/19 ...desired insert should not grow.   Figure 2: Image from http://teachline.ls.huji.ac.il/72682/tutorials...
  7. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ... fluorescent indicators for organellar calcium imaging. Biophysical journal. 2016 Sep 20;111(6):1119-31...2014 Nov 18;7:88. Tian L, Hires SA, Looger LL. Imaging neuronal activity with genetically encoded calcium...
  8. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ...Reports 9: https://doi.org/10.1038/s41598-019-41088-2 Magnus C, Lee P, Bonaventura J, Zemla R, Gomez J, Ramirez...applications. Science 6436: eaav5282. https://science.sciencemag.org/content/364/6436/eaav5282.long Redfern ...
  9. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    Published
    Sept. 27, 2017, 1:23 p.m.
    ...offer guided tours to show people where the lab magic is happening. 3) Volunteer – Step into science education...learning on the job. Try to get an internship at a magazine or newspaper (here’s a partial list). A great ...
  10. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    Published
    April 6, 2017, 1:02 p.m.
    ...Cell Collection at Coriell. More information and images generated from these cell lines can be found in...fluorescent proteins as described here enables live cell imaging to study cellular structures and processes and ...
  11. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    Published
    April 26, 2022, 1:15 p.m.
    ...fluorescent signaling in the presence of dsDNA. Image courtesy of: Ygonaar    Taqman is more sensitive...thousands of PCR reactions using Taqman chemistry (see image, left, of droplet immersion technique, courtesy ...
  12. Transferable Skills: Negotiation

    Type
    Blog Post
    Published
    March 3, 2022, 2 p.m.
    ...confocal microscope. You’re trying to get better images for a paper and she’s trying to get some data for...manuscript is more important to you than collecting your images this week. Even though three examples depend on...
  13. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...which will bind and light up a tagged protein for imaging. Given its popularity and widespread usage, a proteolysis-targeting... avoid AID tags. If your project also involves imaging at some stage, then choosing a HaloTag could pay...
  14. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    Published
    April 4, 2019, 12:53 p.m.
    ... Figure 1: Mobile-CRISPRi's modular components. Image from Peters et al., 2019 with permission.   ...recipient bacteria with an integrated copy of CRISPRi. Image from Peters et al., 2019 with permission.    ...
  15. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    Published
    May 22, 2015, 4:58 p.m.
    ... extend far beyond the laboratory and seriously damage public trust in scientists, experiments involving...technologies with care and humility. Nothing would damage that trust more than an accidental gene drive release...
  16. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...Fluorescent proteins and their applications in imaging living cells and tissues”  Physiol. Rev. 90:1103...et. al. (2011) “Modern fluorescent proteins and imaging technologies to study gene expression, nuclear ...
Showing: 181 - 200 of 414 results