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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 403 results for: mag

Showing: 181 - 200 of 403 results

  2. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ... fluorescent indicators for organellar calcium imaging. Biophysical journal. 2016 Sep 20;111(6):1119-31...2014 Nov 18;7:88. Tian L, Hires SA, Looger LL. Imaging neuronal activity with genetically encoded calcium...

  3. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ...Reports 9: https://doi.org/10.1038/s41598-019-41088-2 Magnus C, Lee P, Bonaventura J, Zemla R, Gomez J, Ramirez...applications. Science 6436: eaav5282. https://science.sciencemag.org/content/364/6436/eaav5282.long Redfern ...

  4. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    Published
    April 26, 2022, 1:15 p.m.
    ...fluorescent signaling in the presence of dsDNA. Image courtesy of: Ygonaar    Taqman is more sensitive...thousands of PCR reactions using Taqman chemistry (see image, left, of droplet immersion technique, courtesy ...

  5. Transferable Skills: Negotiation

    Type
    Blog Post
    Published
    March 3, 2022, 2 p.m.
    ...confocal microscope. You’re trying to get better images for a paper and she’s trying to get some data for...manuscript is more important to you than collecting your images this week. Even though three examples depend on...

  6. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    Published
    April 4, 2019, 12:53 p.m.
    ... Figure 1: Mobile-CRISPRi's modular components. Image from Peters et al., 2019 with permission.   ...recipient bacteria with an integrated copy of CRISPRi. Image from Peters et al., 2019 with permission.    ...

  7. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...which will bind and light up a tagged protein for imaging. Given its popularity and widespread usage, a proteolysis-targeting... avoid AID tags. If your project also involves imaging at some stage, then choosing a HaloTag could pay...

  9. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    Published
    May 22, 2015, 4:58 p.m.
    ... extend far beyond the laboratory and seriously damage public trust in scientists, experiments involving...technologies with care and humility. Nothing would damage that trust more than an accidental gene drive release...

  10. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...Fluorescent proteins and their applications in imaging living cells and tissues”  Physiol. Rev. 90:1103...et. al. (2011) “Modern fluorescent proteins and imaging technologies to study gene expression, nuclear ...

  11. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ...fluorescent label, which can reduce the potential image resolution of super-resolution fluorescence microscopy...Nanobodies: site-specific labeling for super-resolution imaging, rapid epitope-mapping and native protein complex...

  12. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ...   Figure 2: Structure of a retrovirus. Image re-used from Dong & Kantor, 2021 under CC-BY license...   Figure 3: The life cycle of a retrovirus. Image courtesy of Jeremy Luban, MD. Figure 3 depicts...

  13. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    Published
    Nov. 15, 2017, 3 p.m.
    ... appear darker than full vector particles. This image shows that the vast majority of the vectors consist...particles (black arrowhead). Scale bar = 100nm. Image courtesy of David Bell and Svetla Stoilova-McPhie...

  14. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    Published
    April 13, 2021, 1:15 p.m.
    .... Steps 1-1 and 1-2 can be done simultaneously. Image from Li et al., 2021.   The updates involved...gene dosage on N-acetylglucosamine concentration. Image from the Yang lab.   In conclusion, the MUCICAT...

  15. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    Published
    May 20, 2025, 1:15 p.m.
    ...I fell in love with biology because of an image that was honestly quite boring. My Bio 101 professor ...sorting or to visually select cells in a microscopy image for individual analysis. A shuttle vector designed...

  16. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    Published
    May 21, 2024, 1:30 p.m.
    ...would help you do the job as described. Let’s imagine you’re applying to a job that requires ddPCR skills...answer to that is what goes into your cover letter. Imagine you’re applying to a job with a lot of file management...

  17. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ...groups have utilized dCas13 fusion proteins for imaging, tracking, modulating splicing, and regulating ...fluorescent fusion protein dLwaCas13a-NF for in vivo RNA imaging. In short, the Cas13a molecule brings a much anticipated...

  18. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    Published
    Nov. 19, 2020, 2:15 p.m.
    ...-inducible Cre not only halted the neurological damage but completely reversed it, regardless of age or...corrected by CRISPR/Cas9 in wild-type human iPSCs. Image modified from Le et al., 2019. Gene editing ...

  19. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ...with mRNA and charged tRNAs to build a peptide. Image from Mariana Ruiz Villarreal. By the late 1980s... codons are under expressed compared to others. Image from Hiss et al., 2017. Most commonly, scientists...

  20. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    Published
    Oct. 13, 2022, 1:45 p.m.
    ...knock in, base edit, activate, repress, and even image your favorite gene. With so many Cas9 reagents available...target strand based on the sequence of the pegRNA. Image from David Liu.   Activation and repression...
Showing: 181 - 200 of 403 results