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We narrowed to 486 results for: mme

Showing: 181 - 200 of 486 results

  1. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    Published
    Oct. 20, 2022, 1:15 p.m.
    ...These modifications can be done in-lab using commercially available kits or ordered as synthesis modifications... to do-it-yourself with a kit or purchase it commercially synthesized with a label. Not enough options...

  2. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    Published
    Dec. 8, 2016, 3:30 p.m.
    ...resistant to the toxic effects of CcdB. Another commercially available, CcdB-resistant strain is ccdB Survival...these resources further. Feel free to offer your comments and suggestions, and even to submit a guest blog...

  3. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    Published
    Aug. 29, 2024, 1:15 p.m.
    ...just right for your experiment. As always, we recommend checking your cloning via a miniprep and restriction...component plasmid, component name, size, and any comments. You can use the ID to look up the component plasmids...

  5. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type
    Blog Post
    Published
    June 25, 2020, 1:15 p.m.
    ... with our Financial Operations Specialist, Lucy Hammer, about why she did not go. She told me, “I think...favorite response to this question came from Lucy Hammer, our Financial Operations Specialist. She said,...

  6. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    Published
    June 22, 2023, 1:15 p.m.
    ...are always required and lab coats are either recommended or required in BSL-1 and required in BSL-2 labs...with lentivirus, many organizations require or recommend disposable gowns over your lab coats and double...

  7. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    Published
    April 6, 2023, 3 p.m.
    ...antibody. Manning et al. tested a variety of commercially available secondary antibodies and found that...(08)60255-1  Manning, C. F., Bundros, A. M., & Trimmer, J. S. (2012). Benefits and Pitfalls of Secondary...

  9. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ...past the initial round of screening. I STRONGLY recommend that you ask a PI or mentor (your supervisor/ ...not; applications for funding from charities, commercial organizations, or universities may have someone...

  10. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    Published
    Sept. 13, 2016, 2:30 p.m.
    ...that this has precipitated increases to the NIH recommended pay scale – used by many institutions to set ...PMC4304227. 4. Revitalizing biomedical research: recommendations from the Future of Research Chicago Symposium...

  11. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type
    Blog Post
    Published
    Jan. 21, 2015, 1:30 p.m.
    ...come to a meeting with a clear agenda, you will immediately get your supervisor’s attention so you can focus...supervisors might try to intimidate you with negative comments (hostile-aggressives, complainers, know-it all...

  12. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ... liquid containers closed when they aren’t in immediate use. This will prevent spills, but, if any spills...occur, they should be wiped with 70% ethanol immediately. Finally, avoid sticking pipettes into solution...

  13. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    Published
    Sept. 7, 2017, 12:17 p.m.
    ...poured onto the middle of the plate, where it immediately spreads out to form a firm monolayer. The cell...the level of endogenous expression. We highly recommend the use of an electronic pipettor with variable...

  14. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    Published
    Aug. 3, 2023, 1:15 p.m.
    ... Highly expressed markers should be paired with dimmer dyes so they don’t “wash out” the rest of the panel...anything (negative population). It is strongly recommended that when you first run a new panel on a spectral...

  15. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ...during the application process. Many institutions recommend that you reach out to potential advisors before...professors per program. Most applications will recommend that you name two professors in your application...

  16. Transferable Skills: Negotiation

    Type
    Blog Post
    Published
    March 3, 2022, 2 p.m.
    ...hoping that your lab mate would have time to make comments on your manuscript. If you give her this slot,...separately in a future post.   Resources Maria recommends: Getting to Yes: Negotiating Agreement Without...

  17. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...believe that industry postdocs, having already been immersed in an industry environment, have a better understanding...or you want to end up in an industry job, being immersed in that environment sooner rather than later can...

  18. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    Published
    Aug. 21, 2018, 12:31 p.m.
    ...fragments, or genomic DNA. Also remember that commercially available cloning kits can often make the process... some that rely solely on PCR, and a host of commercially available cloning kits that one could choose...

  20. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    Published
    May 22, 2015, 4:58 p.m.
    ...likely capabilities of RNA-guided gene drives in the summer of 2014 (after extensive discussions with biosecurity...sake of laboratory convenience. Disclaimer: Recommendations concerning the appropriate degree of confinement...
Showing: 181 - 200 of 486 results