...color switching, TRICK requires the following components: a GFP protein that binds to the coding region... reporter. Delayed translation is known to be important in development, and Halstead et al. used TRICK...and 3’ UTR. Before beginning experiments, it’s important to verify that these changes do not impact translation...

...be it an organic dye or FP) is one of the most important steps in the design of an imaging experiment.  ...Goedhart on the Addgene blog – outlining various important considerations when choosing a FP, but much of...valid email address).  One may also simply attach important references that serve to further characterize ...

...attention for its powerful promoter of a gene important for methanol usage. This gene promoter, AOX1, ..., From Saccharomyces cerevisiae to human: The important gene co‑expression modules. Biomedical Reports...., Comparative genomics of biotechnologically important yeasts. Proc. Natl. Acad. Sci., 113, 9882-9887...

...it can be important to consider the type of bacteria it is being used in, and how components of the plasmid... bacteria is due to differences in cell wall composition. Cell wall structure Gram positive bacteria, ...

... for favors.  Don’t focus on networking with “important” or senior people. Focus on building relationships...times if you don’t get a response. It breaks an important law of networking--don’t be creepy. There is a...because I want it to be clear that it is completely impossible for me or anyone to stay in touch with all of...

... Lab Archives by LabArchives The single, most important part of lab life is the lab notebook. Lab Archives...always comes first with research! It is always important to know what kind of reagents you work with in...iGreenEarth AMINO is a very simple app that packs important biological information, amino acid abbreviations...

...proteins consuming molecular fuel sources. While the importance of understanding these complex processes can’...degradation under a variety of conditions. They are composed of 4 proteins encoded in a single operon and were...

...that no component of the overall project gets left undone or is duplicated. With each component of the ...responsibility if things go wrong. Here are some important criteria for delegating tasks appropriately: ...

...This is followed by some perhaps surprising, yet important, factors to consider when seeking your dream lab...short-term goals and chat about awesome science. Important factors in the search for a lab Studies show that...postdoc. Although the chosen research field is important, the end goal during this time is to be trained...

...only examined under a few conditions since it is impossible to check all possible combinations of biology...detected signal and therefore high brightness is an important feature of a fluorophore. The key question is ...However, the theoretical brightness ignores several important experimental conditions that are related to the...

... fluorescence, so higher concentrations of the compound can be added to the media. Cellular esterase then...producing a constant supply of substrate. Using this compound to excite the Nano-lanterns, Takai et al. continuously... substrate is required for imaging, and it is important to verify that this substrate does not alter cellular...

...library used. EvolvR: key components The EvolvR system has two key components: the error prone PolI3M that...variants in bacteria. Read on to learn about the key components of EvolvR and its potential applications. Challenges...

...most important resource that Addgene provides the scientific community? Katz: The most important resource...beyond. In your perspective, what do you think are important considerations for the future of open science?...

...tools are not very robust. When using GCE, each component of the experiment system (including media, cell...optimization parameters the same for all three components, meaning that the final protocol may involve ... They provide resources, knowledge, and most importantly, validated protocols (using readily available...

...gamma-retrovirus are so closely related, their required components are very similar. While plasmids containing virus-specific...between the two systems, other, more general, viral components such as a heterologous envelope or a post-transcriptional...713. DOI: 10.3390/v3060677 Varanda C, Félix MDR, Campos MD, Materatski P. An Overview of the Application...

...the target cell or transiently expressed. It’s important to note that for all the applications we discuss... plasmids can induce gene-specific silencing temporarily, long-term, and even inducibly depending on the... virus to which immunity is desired. Instead, components of the virus are now delivered in other forms...

...permanently integrate into the host genome or temporarily express whatever it encodes. This ability to ...allows you to infect cells and deliver cargo, it’s important to understand their differences to choose the ...PMC413431. 2. Bukrinsky MI, et al. “Active nuclear import of human immunodeficiency virus type 1 preintegration...

...consumer-grade components. In this field, often referred to as 'frugal science', existing components are adapted...around the use of commercially available tools and components. Rapid prototyping techniques such as 3D printing...

...aging. For both a screen and a selection, it is important to clearly define what type of factor you are ...CRISPR, drug, etc.), just with an added baseline component. Alternative screening techniques While CRISPR...whole genome), they knock down gene expression temporarily. RNAi libraries are especially useful if you ...

...severely cold conditions. To understand their unique importance, let’s begin with a few basics.  Mesophiles vs...understanding and appreciating their biology. The importance of psychrophilic enzymes From an industrial perspective...scientist from Addgene. The talk highlighted the importance of reagent repositories and the myriad facilities...