...Sonication based lysing protocol Subcellular fractional kit or lysing protocol Not enough target...using a subcellular fractionation kit or a lysing protocol developed for isolating proteins from a specific...errors Search online/ask an expert Review protocol videos for a visual guide to good technique ...

...you’ve gained experience working with similar protocols, and look - they’re seeking someone who has, to...Maybe there was a time you noticed an issue with a protocol and you took it upon yourself to fix the issue...you are in charge of completing many meticulous protocols or tasks, you’ve probably developed an attention...

...Learn our protocol for AAV titration by qPCR Browse our collection of viral vector related protocols Learn...Browse our blog posts related to viral vector protocols and tips Read all of our viral vectors blog posts...

...detailed troubleshooting tips can be found in protocols by Kerppola (2006, 2013). BiFC vs. FRET: When ...visualization of protein interactions in living cells. Nat Protoc 1:1278–1286. https://doi.org/10.1038/nprot.2006.201...Protein Interactions in Live Cells. Cold Spring Harb Protoc 2013:pdb.prot076497. https://doi.org/10.1101/pdb.prot076497...

...and ecology can make for beautifully compelling photos and videos, while molecular, micro, and cell biology...academic to informal writing can difficult.  Photography Medium (high for microscopy) Visually compelling...context via still images. Some things cannot be photographed or need text to properly contextualize. Graphics...

... target is present immediately upstream of a Protospacer Adjacent Motif (PAM), the sequence NGG.  If these... within the editing window (bases 4-8 of the protospacer). While this is a small window, this system can... target sequence has been modified!  The exact protocol may vary depending on your specific experiment...

...producing vectors from plasmids — including the protocols we use in our lab — being able to order high quality...educational resources, from eBooks to guides to protocols, which was why I was so excited to delve into ...Using Pooled Lentiviral CRISPR Libraries. Current Protocols in Molecular Biology, 115, 31.5.1-31.5.13. https...

...using primers (with the desired mutation) in a PCR protocol that amplifies the entire plasmid template. The...simply following the polymerase manufacturers’ PCR protocol. Be sure to adjust the extension time to match... on Addgene.org Browse Our Molecular Biology Protocols Find Empty Vectors for Your Next Cloning Experiment...

...PhosTag gels as described above. Reagents and protocols Sep-OTSλ plasmid - Addgene # 68292 C321.ΔA bacterial...Addgene # 68307 For further technical reference and protocols for use of this system, please reference ‘Rinehart...Western blotting using Phos-tag containing gel." Nat. Protoc. doi 10 (2008). Journal. Resources on the Addgene...

...genome with two-step allelic exchange." Nature protocols 10.11 (2015): 1820. PubMed PMID: 26492139. PubMed...bacteria using homologous recombination." Current protocols in molecular biology 106.1 (2014): 1-16. PubMed...molecular biology reference Watch molecular biology protocol videos ...

...protein characteristics such as the brightness, photostability, maturation time, and acid sensitivity of various...sensors for cAMP feature exceptional brightness, photostability and dynamic range: characterization of dedicated...PMC490022. Shaner, Nathan C., et al. "Improving the photostability of bright monomeric orange and red fluorescent...

... (Cai et al., 2013), incorporates the highly photostable fluorophores mOrange2, EGFP, and mKate2 (red)..., expect to see systems that use newer, more photostable fluorophores and that can be combined with new...Brainbow imaging in zebrafish." Cold Spring Harbor Protocols 2011.1 (2011): pdb-prot5546. Richardson, Alexander...

...DNA sequence and has been suggested to cause cytotoxicity [5]. Second, instead of using a donor-plasmid...“Recombination between inverted loxP sites is cytotoxic for proliferating cells and provides a simple ...sequencing of retroviral insertion sites.,” Nat. Protoc., vol. 4, no. 5, pp. 789–98, Jan. 2009. PubMed ...

...Recombination-Based Method of Genetic Engineering. Nature protocols. 2009;4(2):206-223. doi:10.1038/nprot.2008.227...Bacteria Using Homologous Recombination. Current Protocols in Molecular Biology. 106:V:1.16:1.16.1–1.16.39...and Strains Check Out Other Molecular Biology Protocols ...

...Resources on Addgene.org Basic Molecular Biology Protocols Protocol Videos Molecular Biology Reference Pages  ... critical reagents for many molecular biology protocols, including vector-insert joining for recombinant...

...actually answer your question? If you're using a new protocol or reagents, do you understand all of the steps.... I've found cooking, gardening, hiking, and photography to be very satisfying. Finally, many PFs are...distance runner, gardener, cook, and outdoor photographer.  Additional Resources on the Addgene Blog ...

...from lowered pH due to microbial contamination. Photo by Kaustubh Kishor Jadhav. Mycoplasmas can remain...works in the tissue culture hood while wearing PPE. Photo by Kate Harten DeMaio. Cell lines and reagents...Addgene.org Check out Addgene's written and video protocols ...

...longer available through Kopf). Additional general protocols on stereotaxic surgeries for viral injections ...in nontransgenic and cre driver mice. Current protocols in neuroscience / editorial board, Jacqueline ...using combinatorial viral approaches. Current protocols in neuroscience / editorial board, Jacqueline ...

...the highest frequency of genome editing events. Yamamoto Lab Multiplex CRISPR/Cas9 Assembly Kit: This ... efficiency >70%. CRMAGE is an incredibly fast protocol, with only 5 hours incubation time needed for ...single round of editing, and a subsequent curing protocol that requires only 2-3 hours incubation. Kondo...

... efficiency >70%. CRMAGE is an incredibly fast protocol, with only 5 hours incubation time needed for ...single round of editing, and a subsequent curing protocol that requires only 2-3 hours incubation. E. coli...system has generally low efficiencies or can be proteotoxic when used in Mycobacterial species, Sarah Fortune...