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We narrowed to 515 results for: otos

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Showing: 181 - 200 of 515 results

  1. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    Published
    July 12, 2021, 1:15 p.m.
    ... of a BSL-3 lab. Jesse Bloom’s lab published a protocol and reagents for pseudotyping lentivirus with ...methods to use CRISPR to detect SARS-CoV-2. Other protocols and tools for detection have been made openly ... Pettie D, King NP, Balazs AB, Bloom JD (2020) Protocol and Reagents for Pseudotyping Lentiviral Particles...

  2. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type
    Blog Post
    Published
    March 12, 2020, 1:15 p.m.
    ... and delivers it cargo to certain cell types.  Botond Roska’s lab at the Institute of Molecular and Clinical...types (ex: rods or cones) or cell classes (ex: photoreceptors which encompassed both rods and cones).  Targeted...also expressed. If Cre-mCherry expresses in photoreceptors and horizontal cells, and Cre-dependent-GFP...

  3. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    Published
    March 15, 2016, 2:30 p.m.
    ...drawbacks of different methods (for comprehensive protocols for all of the methods discussed here refer to...staining. Check Out Our In-Depth Fluoresence Titering Protocol Another popular approach utilizes the antibiotic...pseudotyped HIV-1-based lentiviral vectors."Nature protocols 4.4 (2009): 495-505. PubMed PMID: 19300443. 2....

  4. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...Sonication based lysing protocol Subcellular fractional kit or lysing protocol Not enough target...using a subcellular fractionation kit or a lysing protocol developed for isolating proteins from a specific...errors Search online/ask an expert Review protocol videos for a visual guide to good technique ...

  6. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ... process, check out the Addgene AAV Production protocol. Titering Once you’ve produced, harvested, and...process, refer to the Addgene AAV Titration by qPCR Protocol. If you’re interested in the ddPCR titering process...Transfer PlasmidAAV Variables That Matter AAV protocols on addgene.org  AAV Titration by qPCR Using SYBR...

  7. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    Published
    Sept. 7, 2017, 12:17 p.m.
    ...figure 1.   Find Additional Useful Protocols on Addgene's Protocol Pages Agarose cloning method procedure...Vectors Resources on Addgene.org Check Out Our Protocols Pages Browse Fluorescent Protein Plasmids Find...

  8. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    Published
    Dec. 10, 2020, 1 p.m.
    ...genome, removing the risk of oncogenicity or genotoxicity seen with other vectors (Lukashev et al., 2016...along with a description of their experimental protocol. The three plasmids which have been deposited ...Gonçalves and Ventura labs’ plasmids, including protocols, check out the plasmids at Addgene: pAdSh.PGK....

  9. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    Published
    April 26, 2022, 1:15 p.m.
    ...Resources on Addgene.org Protocol: Polymerase Chain Reaction (PCR) Protocol: How to Design a primer  ...variants based on real-time LightCycler PCR. Curr Protoc Hum Genet. 2014;80:7.21.1-7.21.. Published 2014...

  11. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    Published
    Aug. 18, 2015, 2:30 p.m.
    ...of the genome and located immediately 5’ to a protospacer adjacent motif (PAM) sequence. Co-delivery of...a given phenotype. Image Credit: www.flickr.com/photos/72005145@N00/5600978712/ What CRISPR-based ...Using Pooled Lentiviral CRISPR Libraries. Current Protocols in Molecular Biology 115: . https://doi.org/10.1002...

  12. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ...Learn our protocol for AAV titration by qPCR Browse our collection of viral vector related protocols Learn...Browse our blog posts related to viral vector protocols and tips Read all of our viral vectors blog posts...

  13. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

    Type
    Blog Post
    Published
    Aug. 27, 2020, 1:15 p.m.
    ...detailed troubleshooting tips can be found in protocols by Kerppola (2006, 2013). BiFC vs. FRET: When ...visualization of protein interactions in living cells. Nat Protoc 1:1278–1286. https://doi.org/10.1038/nprot.2006.201...Protein Interactions in Live Cells. Cold Spring Harb Protoc 2013:pdb.prot076497. https://doi.org/10.1101/pdb.prot076497...

  14. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    Published
    July 1, 2022, 1:15 p.m.
    ...and ecology can make for beautifully compelling photos and videos, while molecular, micro, and cell biology...academic to informal writing can difficult.  Photography Medium (high for microscopy) Visually compelling...context via still images. Some things cannot be photographed or need text to properly contextualize. Graphics...

  15. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    Published
    Jan. 11, 2018, 3:29 p.m.
    ...you’ve gained experience working with similar protocols, and look - they’re seeking someone who has, to...Maybe there was a time you noticed an issue with a protocol and you took it upon yourself to fix the issue...you are in charge of completing many meticulous protocols or tasks, you’ve probably developed an attention...

  16. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    Published
    July 15, 2025, 1:15 p.m.
    ...them. DNA or RNA is pulled through the pore via motor proteins. When each nucleotide passes through the...Next‐Generation sequencing Technologies. Current Protocols in Molecular Biology, 122(1). https://doi.org/... Molecular Biology Reference Sequence Analyzer Protocol: Sequence Analysis of a Plasmid ...

  17. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    Published
    July 30, 2024, 1:15 p.m.
    ...producing vectors from plasmids — including the protocols we use in our lab — being able to order high quality...educational resources, from eBooks to guides to protocols, which was why I was so excited to delve into ...Using Pooled Lentiviral CRISPR Libraries. Current Protocols in Molecular Biology, 115, 31.5.1-31.5.13. https...

  18. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    Published
    Oct. 13, 2022, 1:45 p.m.
    ... target is present immediately upstream of a Protospacer Adjacent Motif (PAM), the sequence NGG.  If these... within the editing window (bases 4-8 of the protospacer). While this is a small window, this system can... target sequence has been modified!  The exact protocol may vary depending on your specific experiment...

  19. Students Invented a New Diagnostic for Lyme Disease — and a Tool for CRISPR Researchers

    Type
    Blog Post
    Published
    May 12, 2026, 1:15 p.m.
    ...our attention when they cited our primer design protocol — then blew our minds with their project, called..., Miao, X., Streithorst, J. A., Granados, A., Sotomayor-Gonzalez, A., Zorn, K., Gopez, A., Hsu, E., Gu...Matagne, A., Aucher, W., Verdon, J., Paoli, E., Ducrotoy, J., Octave, S., Avalle, B., Maffucci, I., & Padiolleau-Lefèvre...

  20. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ...genome with two-step allelic exchange." Nature protocols 10.11 (2015): 1820. PubMed PMID: 26492139. PubMed...bacteria using homologous recombination." Current protocols in molecular biology 106.1 (2014): 1-16. PubMed...molecular biology reference Watch molecular biology protocol videos ...
Showing: 181 - 200 of 515 results