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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 628 results for: rel

Showing: 181 - 200 of 628 results

  1. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    Published
    Sept. 3, 2015, 2:30 p.m.
    ... Chen co-founded Addgene in 2004. They worked tirelessly to get Addgene from a concept to an up and running...job were important parts of team buidling - they relieved some of the pressure of start up life and maintained...“work hard, play hard.” Part of the reason we sincerely like spending time together is that when it’s ...employees. The teambuilding culture helps us build relationships and thereby work better together.I realize I...encourage everyone to make an effort to build relationships with their colleagues. It can be easy to get...done everything from kayaking, to field day, to relay triathlons, to 5Ks. Many of these examples we’ve...conversation to get people excited!    Laura Veckerelli is the Administrative and Operations Coordinator...

  2. Scientist Networking: What is an Informational Interview?

    Type
    Blog Post
    Published
    July 1, 2014, 3:42 p.m.
    ... more open to developing a relationship. The time to develop relationships is before you need them (well...academia often means that scientists-in-training rarely get the opportunity to learn about the myriad of...doing interesting things. I call this building relationships because networking has gotten a bad reputation...Scientists enjoy learning new things. Building new relationships is all about learning new things from other ...networking for a job connection with ongoing relationship building through Informational Interviews. Making...is always best to meet in person to develop relationships, but if it is not possible consider a phone ...

  3. Celebrate National Book Month with Addgene

    Type
    Blog Post
    Published
    Oct. 3, 2019, 12:47 p.m.
    ...Sacks "Neurologist Oliver Sacks examines the relationship between the human brain and music: how we perceive.... Sacks, Musicophilia includes case studies of relevant neurological atypicalities, including a patient...and fascinating, all without compromising on the relevant science."     - Randall Carter, Contracts Administrator...roles microbes play in our ecosystem, examining relationships between microbes and insects, mammals, and plants...Director of Quality Control Jonathan Strange & Mr. Norrell by Susanna Clarke "A quasi-historical/fantasy fiction... had a few reasons for launching this one. On a purely selfish level, I wanted to be challenged to read...responses ranged from “one of my favorite books” to “I barely got through it.”  To date, we’ve read 76 books ...

  4. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    Published
    March 3, 2020, 2:15 p.m.
    ...base editors in vivo and achieved therapeutically relevant editing efficiencies in the mouse brain, liver...Physiol 2018. PubMed PMID: 30819783. Light up CreLite: a red-light inducible optogenetic tool for precision...lineage tracing Article contributed by Erin Sanders CreLite, designed by the Eisenhoffer lab, is a new tool...systems (ex: tamoxifen). As the name suggests, CreLite combines the Cre/lox system with optogenetics. ...studies. Eisenhoffer and his colleagues evaluated CreLite for cell tracing using the transgenic zebrafish...combination of fluorescent proteins expressed. CreLite-mediated recombination was found in both deep and...the breadth and versatility of this tool. Find CreLite at Addgene. Yen et al., bioRxiv 2019. DOI: 10.1101...

  5. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type
    Blog Post
    Published
    June 26, 2018, 1:33 p.m.
    ...NFIB- and NFIC-related human syndromes. As therapies are developed for specific NFI-related genetic disorders...formerly occupied by the Arg sidechain. Although not related to this rescue function, GdnHCl was approved by...binds to and inhibits a potassium channel, causing release of acetylcholine. Given its unique molecular biology...PubMed PMID: 9514755. Richard Gronostajski: NFI-related diseases of neural development Richard Gronostajski...VUSs will enable more accurate diagnosis of NFIX-related syndromes (Sotos-type 2 and Marshall-Smith) and...these will also allow better treatment of these related syndromes. Many thanks and best of luck to the...

  6. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    Published
    Nov. 29, 2022, 2:15 p.m.
    ...racial bias from work being done today, is a nearly entirely separate effort from acknowledging its existence...dependencies  Take, for instance, a recent preprint released by the Boehm and Beroukhim labs at the Broad Institute...tumors, which can lead to new cancer targets and related biomarkers . Data from such screens are often uploaded...Unexpected results Their results showed 96 strongly correlated genetic dependencies that were clearly associated... they looked for a biological explanation, a relationship between the genetic dependencies they identified... ancestries. While there were some exciting relationships identified, 48% were found to be artificial,...bias into their experiments instead of a true correlation.   It turned out there was indeed a bias, and...

  7. Great Results Start with Great Standard Curves

    Type
    Blog Post
    Published
    Aug. 15, 2024, 1:15 p.m.
    ...could be because there is no correlation or it could mean that the relationship is non-linear and you should...down the hall from you have in common? All of them rely on standard curves in their day-to-day work. Indeed...concentrations in food; and your friend the grad student relies on a standard in their bicinchoninic acid assay...of determination, R2. R2 is the square of the correlation between the actual values and the predicate values...fit and 0 indicating that there is no linear relationship between the observed and predicted values. This...Additional considerations When running assays that rely on a standard curve, it is critical that the unknown...

  8. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    Published
    July 7, 2020, 1:15 p.m.
    ...re inefficient compared to CRISPR/Cas9 edits that rely on double strand DNA breaks. Due to this inefficiency...2018). TLDR: The ACE reporter from the Harris Lab relies on the restoration of a frame-shift in mCherry ...quantifies APOBEC-Cas9 efficiency in real-time, it relies on DSBs which is not ideal for monitoring single...APOBEC-Cas9 editisome complexes. The eGFP reporters rely on the correction of point mutations in eGFP that...base-editing activity The reporters mentioned above all rely on GFP fluorescence and cell sorting using FACS ...enriches for base editing events in vivo without relying solely on GFP (Katti et al., 2020). They named ...reporter system for base-editing events in vivo. As GO relies on the correction of a mutated start codon to initiate...

  9. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    Published
    Oct. 31, 2019, 1:06 p.m.
    ...membrane. Vampirovibrio chlorellavorus will not grown in culture alone and relies on its algae host for ...ant’s body. The fruiting bodies then rupture and release spores onto the forest below for the next unsuspecting...stumble upon. Vampire bacteria Vampirovibrio chlorellavorus is a predatory bacteria that attaches to the...adult worms develop in the digestive tract and release eggs in the bird feces, which get eaten by another... the predatory cyanobacterium Vampirovibrio chlorellavorus." PeerJ 3 (2015): e968. PubMed PMID: 26038723...

  10. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    Published
    June 12, 2018, 11:40 a.m.
    ...difficult to consolidate every conceivable chromatin-related plasmid in a collection that grows on a daily basis... basis, the lists below provide a snapshot of relevant resources and a good jumping off point. The following... Yi Zhang Leonard Zon  You!     Chromatin-related genes with plasmids available from Addgene The ...directly on our website. In-exhaustive Chromatin-related genes with plasmids on Addgene.org AGO1 BRCA1...from Addgene Many articles reporting chromatin-related research describe the production of plasmids that...2018 Feb 13. pii: 1722491115. Addgene blog posts related to chromatin This is not the first Addgene blog...

  11. Finding Your Perfect Job After University

    Type
    Blog Post
    Published
    Jan. 12, 2016, 3:30 p.m.
    ... Sutton, located on the edge of London to gain relevant experience. I applied for this summer position...because I knew the tests worked and would produce a reliable result. This is not to say the role was without...left me optimistic that I would find a genetics related role soon after finishing my MSc. The masters program...large scale data analysis, as this is increasingly reliant on programming skills which are not my strong point...I had received all the training and experience relating to good manufacturing practice (GMP) and good ...downside to large commercial companies which are reliant on contracts with clients is that, when those contracts...

  12. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    Published
    Nov. 6, 2015, 3:30 p.m.
    ...and their successes. Although all mentorship relationships are valuable and highly recommended, a mentor...mentor creates a closer and more approachable relationship for the mentee, where (s)he feels truly understood...Scholars need strong proactive mentors who have relevant expertise and skills, positive disposition and..., mentors and mentees often developed close relationships with a level of sympathy that is difficult to...confidence. These improvements, combined with the relationships fostered through the IMP have motivated many...-Harvard Medical School, where she studies age-related macular degeneration. She is also a blogger who...

  13. 2023 and 2024: Reflections and Looking Ahead

    Type
    Blog Post
    Published
    Jan. 23, 2024, 2:15 p.m.
    ...targeting integrins, will be released early this year.  In September, we released the fourth edition of our...the recent release of the Spanish version of our CRISPR 101 eBook and the upcoming release of the second...services available in South Korea.  In addition, we released the MORF collection, a comprehensive transcription...

  14. Increasing Your Work's Visibility with Addgene: Citation, Search, and Collections

    Type
    Blog Post
    Published
    March 10, 2016, 3:30 p.m.
    ... of their work (H-index for instance), but these rely upon other researchers being able to find their ... for you to find the plasmids and publications relevant to your research interests. We do this by promoting...and money. A quick note -  While it is tempting to rely on the citation number when comparing two plasmids...video: Please remember that the accuracy and reliability of our search results depends upon you, our depositors...visibility in a few other ways. Every quarter we release a Newsletter where we spotlight some of the plasmids...

  15. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    Published
    March 26, 2019, 1:40 p.m.
    ...applications. We recently released a new variant of jGCaMP7 (keep reading) and will be releasing more variants in...viral vectors. Here are some of the AAV we have released so far in 2019. You can also browse our entire...inhibitors that respond to blue light. We recently released a constitutive (pAAV-Syn-Archon1-KGC-GFP-ER2) ...coming soon! These vectors listed below should be released at Addgene in the next 6 months, pending quality...

  16. Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation

    Type
    Blog Post
    Published
    Nov. 8, 2018, 3:47 p.m.
    ...slight or large variations depending on how closely related these species are. If a gene was moved exactly ...exactly as is from one organism to a distantly related organism, the gene may not express well because of these...saw that a percent sequence identity below 85% correlates with synthetic sequences. The researchers then...growing trend of sourcing genes from distantly related organisms. In 2006, less than 1% of the genes deposited...applications in monitoring the accidental or intentional release of engineered organisms. “Is [the organism] engineered...

  17. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    Published
    March 14, 2019, 1:54 p.m.
    ...describe a process that begins with cell lysis to release AAV particles with subsequent purification steps...also possible for AAV vectors to be spontaneously released from the producer cells into the supernatant during...took a closer look at the AAV vectors that are released from cells during production (Maguire et al., ...current purification protocols for isolating exo-AAVs rely on differential centrifugation steps to separate...et al. 2018). The structure of exosome AAVs is related to the use of microvesicles as a vehicle for drug...

  18. DAP arrays for multiplex gene editing

    Type
    Blog Post
    Published
    July 7, 2022, 1:15 p.m.
    ...Although CRISPR-Cas12a, with abilities to process and release its own gRNAs from a single array, has been a top...array. After expression, the individual gRNAs are released by endogenous tRNA processing machineries, specifically...promoter, which is followed by tRNA processing to release individual gRNAs for gene editing. They named this...the strength as a promoter and the activity to release gRNAs from DAP array.7 Using DAP arrays with a ...architectures, including previous tRNA-gRNA arrays, that rely on an upstream lengthy promoter to express the gRNA...

  19. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    Published
    Aug. 7, 2014, 1:58 p.m.
    ...no better place for a scientist to make new relationships than at scientific conferences. Conferences ...this is a great ice breaker. Of course, real relationships go further and grow over time, but being interested...fun, memorable meal is a great way to build relationships. Make a list of experimental problems or questions...work – casual socializing is how people build relationships that are memorable. It isn’t always appropriate...Thesis Whisperer "Not Networking - Building Relationships for Success" webinar with Joanne Kamens via ...

  20. Will You Be My Mentor? Finding and Asking for Mentoring Support

    Type
    Blog Post
    Published
    Feb. 4, 2014, 3:50 p.m.
    ...watch my webinar, “Not Networking 101-Building Relationships for Success”. Choose 1-2 tactics to try. One...be a bit random because you never know which relationships will take off, or who your new contact will ...current organization but also important to build relationships outside your organization. The mentor in your... doing it again next month?” Some mentoring relationships grow naturally out of friendships or work interactions...other ways they can help you grow. Mentoring relationships can last for an hour or for a year. Some click...
Showing: 181 - 200 of 628 results