...colored product that accumulates over time which is relative to the amount of enzyme present in each well. ... categories: Quantification - used to quantify relative amounts of protein. Includes: Western blot ELISA... western blots or ELISAs, are used to quantify relative amounts of protein. Western blot Western blots...proteins separated by size on an SDS-PAGE gel. Though relative quantification can be done, western blots primarily...referred to as housekeeping genes). This allows for relative comparison of expression between different samples... induced to express antibodies. Antibodies are released into the growth medium and can either be used ...antibody fragments (shown in Figure 2) to completely unrelated molecules. Examples include: scFv - Single-chain...

...hundreds of available enzymes, many of which are relatively cheap. They also cut specific target sequences...and choice of restriction site. Because of the relatively long stretches of base pairing in the annealed...called TOPO® cloning or TA cloning, is a method that relies on the hybridization of the complementary base ...Independent Cloning Ligation Independent Cloning (LIC) relies on the 3'-5' exonuclease activity of T4 DNA polymerase...

...practically this isolation is only feasible for relatively short DNA molecules. In order to isolate a particular...most plasmids are replicated in E. coli and are relatively small (∼3000 - 6000 basepairs) to enable easy...Because bacteria are easy to grow in a lab, divide relatively quickly, and exhibit exponential growth rates..., and thus the plasmid exists in a symbiotic relationship with the host cell. Like the bacterial chromosomal...recommend checking out these two Addgene blog posts relating to common E. coli lab strains and E. coli strains...specialized for protein expression for additional strain-related information and a more extensive strain list. Strain...endA1 hsdR17(rk-, mk+) phoA supE44 thi-1 gyrA96 relA1 tonA JM109 Addgene; Promega e14-(McrA-) recA1 endA1...

... to Top Glossary Plasmid Type Element Delivery relative to transgene Purpose Envelope VSVG in trans Vesicular...

...insert donor DNA at “landing sites” defined by relatively short sequences. Some have been paired with CRISPR...high-fidelity homology directed repair (HDR) pathway. HDR relies on copying DNA from a matching template to accurately... transduced cell population, you will look for relevant hits using NGS technology. For more detail on ...As this reporter is degraded by Cas12a, it will release a quantifiable signal that indicates the presence... J., & Van Der Oost, J. (2012). CRISPR immunity relies on the consecutive binding and degradation of negatively...

...firing through stimulating phospholipase C, which releases intracellular calcium stores. There are currently... DREADDs in experiments in this species may be entirely due to back-metabolism of CNO to clozapine, demonstrating..., Jin J (2015). The First Structure–Activity Relationship Studies for Designer Receptors Exclusively Activated...460. PMID: 15765260 (Link opens in a new window) Farrell MS, Pei Y, Wan Y, Yadav PN, Daigle TL, Urban DJ...

...the wild-type opsin of each and some examples of relevant variants. This list is not exhaustive - please...phototropin 1 LOVETRAP reversibly sequester and release proteins from cellular membranes using light. light-induced...light-controlled gene expression. These plasmids rely on the engineered two-component regulatory system...

...any of our eBooks to learn about popular topics related to our collections. Titles include: Plasmids 101...Read about plasmids in Addgene’s repository , and related molecular biology techniques. Topics include: Genome...

...start learning about various scientific concepts related to our plasmid collection. Find links to the guides...

...non-enveloped double-stranded DNA viruses. They are not related; however, AAV requires the presence of adenoviral...

...Both types of screen employ a selection method relevant to the phenotype being studied. Examples of published...

...particles produced during a lytic cycle are quickly released through cell lysis. Episomal stability enables...