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  1. Viral Production

    Type
    Collection
    ...of AAV preparations is assayed by comparing the relative stoichiometric ratios of the viral capsid proteins...SYPRO Ruby staining and the molecular weight and relative intensity of the viral capsid proteins are analyzed...Viral capsid proteins VP1, VP2, and VP3 are shown relative to the total protein present in the sample. Sterility...vectors consist of full particles (white arrowhead) relative to empty particles (black arrowhead). Scale bar...

  3. Viral Vectors

    Type
    Collection
    ...transfer in clinical trials. These viruses have a relatively high transduction efficiency, though this infectivity...These engineered viral genomes are also modified (relative to natural viruses) for safety through the removal...vector plasmids available at Addgene and other related resources. Special...

  4. Antibody Guide

    Type
    Collection
    ...colored product that accumulates over time which is relative to the amount of enzyme present in each well. ... categories: Quantification - used to quantify relative amounts of protein. Includes: Western blot ELISA... western blots or ELISAs, are used to quantify relative amounts of protein. Western blot Western blots...proteins separated by size on an SDS-PAGE gel. Though relative quantification can be done, western blots primarily...referred to as housekeeping genes). This allows for relative comparison of expression between different samples... induced to express antibodies. Antibodies are released into the growth medium and can either be used ...antibody fragments (shown in Figure 2) to completely unrelated molecules. Examples include: scFv - Single-chain...

  5. Retrovirus Plasmids

    Type
    Collection
    ...called γ-retroviruses. γ-Retroviruses can package relatively high amounts of DNA (up to ∼8 kb), and infection...

  6. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...contained at lower biosafety levels and elicit relatively low immunological effects in vivo . While AAVs...virus, in order to propagate. This is due to the reliance of the AAV on certain exogenous gene products ...

  7. TALEN Guide

    Type
    Collection
    ...Intuitively, one would expect that assembling relatively small, repeat-laden DNA regions into a single...at Massachusetts General Hospital also recently released a TALEN kit through Addgene. This kit, first described...

  8. Rett Syndrome

    Type
    Collection
    ...diagnostic criteria, defined as the presence of relatively normal early development, regression with a loss... of treatments and cures for Rett Syndrome and related MECP2 disorders. The RSRT has a comprehensive, ...new window) PMID: 10508514 Archer et al. 2007. Correlation between clinical severity in patients with Rett...display tissue- and cell-specific differences and correlate with neuronal maturation. Hum Mol Genet . 11, ...

  10. Bacterial Expression Systems

    Type
    Collection
    ...researchers also use bacteria like E. coli and their relatively well understood biology to answer many interesting...

  11. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ... for genetic screening experiments, due to the relative ease of designing gRNAs and the ability to modify...3rd 4 2,852 Bonifacino Lab Human ubiquitination-related proteins CRISPR KO library 174592 Knockout Human...

  12. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...of orientation of the protospacer sequence/PAM relative to the top or bottom strand. Determine the reverse...identification, and characterization. This strategy relies on cellular delivery of a pair of chimeric single...limiting dilution prior to performing this step to reliably obtain approximately 30 cells per 96-well plate...coli cells and plate onto LB agar plates with the relevant antibiotic. Select multiple colonies, mini-prep...RNA, perform RT-qPCR for gene expression of the relevant gene 7,8 . To evaluate the protein, perform an...an immunoblot using an antibody against the relevant protein 9 . References This protocol is excerpted...

  13. Plasmids for Stem Cell Research

    Type
    Collection
    ...to generate iPSCs. The generation of iPSCs is relatively simple in concept: ectopically express a cocktail...multiple cell types, but are typically limited to related cell lineages. Pluripotent embryonic and multipotent...technology removes the possible ethical concerns related to embryonic stem cell use and has the major advantage...

  14. Immunology Research Plasmids and Resources

    Type
    Collection
    ...transcript 1L - RELB v-rel reticuloendotheliosis viral oncogene homolog B I-REL, IREL RFX5 regulatory ...RASGRP3 RAS guanyl releasing protein 3 (calcium and DAG-regulated) GRP3, KIAA0846 RELA v-rel reticuloendotheliosis...CALDAG-GEFII, MGC129998, MGC129999, RASGRP, V, hRasGRP1 RELA v-rel reticuloendotheliosis viral oncogene homolog ...like beta FIZZ1, FIZZ2, HXCP2, RELM-beta, RELMb, RELMbeta, XCP2 RLN1 relaxin 1 H1, RLXH1, bA12D24.3.1, bA12D24.3.2...polypeptide-related sequence A FLJ60820, MGC111087, PERB11.1 MICB MHC class I polypeptide-related sequence...CST-29 CRH corticotropin releasing hormone CRF CRHR1 corticotropin releasing hormone receptor 1 CRF-R,...ESRRA estrogen-related receptor alpha ERR1, ERRa, ERRalpha, ESRL1, NR3B1 ESRRB estrogen-related receptor beta...

  15. Ras Pathway

    Type
    Collection
    ...guanine nucleotide releasing factor RASGRP RASGRP1 RASGRP2 RASGRP3 RASGRP4 RAS guanyl releasing protein RASSF...for clarity and does not indicate a specific relationship. The content and map for this page were generated...FGFR4 Fibroblast growth factor receptor FLT3 Fms related tyrosine kinase 3 FNT FNTA FNTB Farnesyltransferase...methyltransferase INSR Insulin receptor INSRR Insulin receptor-related receptor IRS IRS1 IRS2 Insulin receptor substrate...Phosphatase and tensin homolog RAC RAC1 RAC2 RAC3 Ras-related C3 botulinum toxin substrate (rho family, small...v-ral simian leukemia viral oncogene homolog (ras related) RALBP1 RalA binding protein 1 RALGDS Ral guanine...

  16. COVID-19 Resources

    Type
    Collection
    ...the knowledge and understanding of COVID-19 and related coronaviruses. To assist with this effort Addgene...plasmids available that may be useful for COVID-19 related research. Additionally, we have linked to collections...articles, protocols, and other resource collections related to COVID-19 that may be of use to scientists. Background...entry of SARS-CoV and SARS-CoV-2. Find plasmids related to these genes below. ID Plasmid Description Industry... in the body. For a full list of cytokines and related plasmids see our Immunology Resource Guide . Return...Simple, portable, and inexpensive platforms to reliably detect nucleic acids at the atomolar level. For...publishers have allowed free access to articles related to SARS-CoV-2 research during the current outbreak...

  17. Nuclear Receptor Signaling Atlas (NURSA) Plasmid Guide: Nuclear Receptors

    Type
    Collection
    ...estrogen-related receptor alpha ESRRB Plasmids ESRRB, DFNB35, ERR2, ERRb, ESRL2, NR3B2 estrogen-related receptor...Engineering Guide Empty-Backbone Resource Image is released to public domain by author. Source: Wikipedia,...Plasmids ESRRG, ERR3, ERRgamma, NR3B3 estrogen-related receptor gamma HNF4A Plasmids HNF4A, RP5-1013A22.1...MGC119326, MGC119329, RZR-ALPHA, DKFZp686M2414 RAR-related orphan receptor A RORB Plasmids RORB, NR1F2, ROR-BETA...ROR-BETA, RZR-BETA, RZRB, bA133M9.1 RAR-related orphan receptor B RORC Plasmids RORC, RP11-98D18.11-001...001, NR1F3, RORG, RZR-GAMMA, RZRG, TOR RAR-related orphan receptor C RXRA Plasmids RXRA, NR2B1 retinoid...

  18. Cre-lox system

    Type
    Collection
    ...Mammalian, Zebrafish Eisenhoffer 131782 pME-CreLite CreLite system components, PhyBdeltaCreC and PIF6CreN...Tol2 kit) Zebrafish Eisenhoffer 131783 pTol2-CreLite CreLite; Tol2 destination vector with mTagBFP2 Zebrafish...Zebrafish Eisenhoffer 131785 pAAV-CreLite CreLite system components, PhyBdeltaCreC and PIF6CreN, in AAV ...transfer vector Cbh AAV Eisenhoffer 131786 pLenti-CreLite CreLite system components, PhyBdeltaCreC and PIF6CreN...2A-Cre CAG Mammalian Breunig 131780 pCS2+PIF6CreN CreLite - Red light-inducible Cre; N terminus Cre fused...Mammalian, Zebrafish Eisenhoffer 131781 pCS2+PhyBΔCreC CreLite - Red light-inducible Cre; C terminus Cre fused...containing FLEx technology. Read the genOway press release for more information. Empty backbones to regulate...

  19. Genetic Code Expansion

    Type
    Collection
    ...coli azi-donorleucine (Anl) Bacterial ATG David Tirrell 62598 pKPY514 phenylalanyl-tRNA synthetase subunit...p-azido-L-phenylalanine (Azf) C. elegans ATG David Tirrell 62599 pKPY197 phenylalanyl-tRNA synthetase (CePheRS...p-azido-L-phenylalanine (Azf) C. elegans ATG David Tirrell 63177 pMarsL274G methionyl-tRNA synthetase (L274GMmMetRS...musculus azidonorleucine (Anl) Mammalian ATG David Tirrell 64915 pMAH-POLY tyrosyl-tRNA synthetase E. coli...musculus azidonorleucine (Anl) Mammalian ATG David Tirrell 91705 pSupAR-MbPylRS(DiZPK) PylRS M. barkeri photocrosslinkers...using genetic code expansion without buildup of prematurely truncated protein. Ryan Mehl 197656 BL21(DE3)... by tolC, and tyrU gene replaced by GentR) and release factor 1. Expresses archaeal MjTyrRS/tRNA pair ...

  20. mTOR Pathway

    Type
    Collection
    ...signaling of mTOR, a key metabolic regulator, correlates with poor prognosis in many cancers....Enhanced signaling through either mTORC1 or mTORC2 correlates with poor prognosis in many cancers, including...for clarity and does not indicate a specific relationship. The content for this page was generated with...tensin homolog Rag RRAGA RRAGB RRAGC RRAGD Ras-related GTP binding Raptor Also known as RPTOR; Regulatory...
Showing: 1 - 20 of 60 results