We narrowed to 262 results for: rigi
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TypeBlog PostPublishedMarch 12, 2024, 1:15 p.m....functional relevance following a set hierarchy. MoClo originally developed from Golden Gate Cloning, in which ...
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Adapting Toehold Switches to Detect Zika Virus
TypeBlog PostPublishedAug. 30, 2016, 2:30 p.m....-specific diagnostics for the Ebola virus. Our original proof-of-concept work was exciting because it ... -
Tips for Titering Your Lentiviral Preps
TypeBlog PostPublishedMarch 15, 2016, 2:30 p.m....PCR product that comes from amplification of the original plasmid which can over-inflate results. A packing... -
Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015
TypeBlog PostPublishedDec. 1, 2015, 3:30 p.m....Szostak. Dr. Szostak’s lab continues its work on the origin of cellular life. His lab has long hypothesized... -
Illuminating Epigenetics with A FRET Based Biosensor
TypeBlog PostPublishedNov. 19, 2015, 3:30 p.m....expressed in normal individuals (in a parent-of-origin-specific manner), but the expression or silencing... -
How-to: 5 Steps to a Great Panel Discussion
TypeBlog PostPublishedOct. 22, 2014, 1:57 p.m....This post was originally published on LinkedIn. Follow Addgene on LinkedIn for repository news and updates... -
Production of Virus in Insect Versus Mammalian Cells
TypeBlog PostPublishedNov. 5, 2024, 8:32 p.m....human neuronal cells by human adenoviruses and the origin of HEK 293 cells. The FASEB Journal, 16(8), 869... -
Viral Vectors 101: Producing Your rAAV
TypeBlog PostPublishedJuly 16, 2024, 1:15 p.m.... our Viral Vectors 101 series! This post was originally written by Didem Goz Ayturk and updated by Ina... -
Pooled Library Amplifications
TypeBlog PostPublishedMay 30, 2024, 1:30 p.m.... Addgene strives to distribute aliquots of the original library whenever possible. In this blog, we’ll... -
Twenty Years of Addgene Sharing: CRISPR
TypeBlog PostPublishedApril 18, 2024, 1:15 p.m....2014 to 2023. As you can see, nucleases, the original CRISPR tool, remain the most popular deposited... -
AAV Vector Quality Control: Going the Extra Mile with NGS
TypeBlog PostPublishedSept. 12, 2017, 1:44 p.m....identify AAV-44632, the contaminant we mixed into the original AAV sample at 5%. Detecting 5% contamination is... -
Fluorescent Proteins 101: Fluorescent Protein Timers
TypeBlog PostPublishedMay 4, 2017, 2:30 p.m....of protein fusions or expressed independently) originally fluoresce green or blue and, when they mature... -
10 Basic tips for mammalian cell culture
TypeBlog PostPublishedJuly 12, 2018, 1:09 p.m....ensures that you have many cells stored in their original condition and can replace your working stock if... -
CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments
TypeBlog PostPublishedFeb. 7, 2019, 2:16 p.m....generated info for your own purposes, or delete the originals if desired. It works as a whole or in part. For... -
Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells
TypeBlog PostPublishedDec. 10, 2020, 1 p.m....Originally published Sep 30, 2014 and last updated Dec 10, 2020 by Benoit Giquel. Adenoviral vectors... -
Polymerase Chain Reaction Overview and Applications
TypeBlog PostPublishedAug. 10, 2021, 1:15 p.m....statistics to determine the concentration of the original template DNA in the sample. Addgene currently... -
Light Sheet Fluorescence Microscopy
TypeBlog PostPublishedJuly 16, 2015, 2:30 p.m....was first pioneered by Voie and colleagues and originally named orthogonal-plane fluorescence optical sectioning... -
Plasmids 101: Multicistronic Vectors
TypeBlog PostPublishedSept. 9, 2014, 8:20 p.m.... of two proteins from a single mRNA. IRES was originally discovered in poliovirus RNA, where it promotes... -
Getting to Know Expansion Microscopy
TypeBlog PostPublishedJune 20, 2023, 1:15 p.m.... evenly in all directions to several times its original size. Depending on the protocol, samples can be... -
CRISPR 101: RNA Editing with Cas13
TypeBlog PostPublishedJuly 31, 2020, 12:30 p.m....Originally published Nov 30, 2017 and updated Jul 31, 2020. Cas13 enzymes are quickly becoming major ...