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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 8 results for: rigi

Showing: 1 - 8 of 8 results

  1. CRISPR Library Amplification

    Type
    Protocol
    ...libraries may require modifications dictated by the originating laboratory for optimal results. If you obtained...the elements required for bacterial propagation (origin of replication and antibiotic selection). This ...option is to perform a gel extraction of either the original sample or the amplified sample, followed by reamplification...

  2. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ... Map of pLKO.1 containing an shRNA insert. The original pLKO.1-TRC cloning vector has a 1.9kb stuffer ...Self-inactivating long terminal repeat. f1 ori f1 bacterial origin of replication. Amp R Ampicillin resistance gene...plasmid in bacterial cells pUC ori pUC bacterial origin of replication. 5’LTR 5’ long terminal repeat. ...

  3. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...antibody-expressing plasmids containing the SV40 origin of replication use a HEK293 line stably expressing...antibody-expressing plasmids containing the Epstein-Barr virus origin of replication use a HEK293 line stable expressing...

  6. Weighing Reagents Protocol

    Type
    Protocol
    ...much material, don’t put any extra back into the original container. Dispose of the excess material into...
Showing: 1 - 8 of 8 results