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We narrowed to 262 results for: rigi

Showing: 1 - 20 of 262 results

  1. Plasmids 101: Origin of Replication

    Type
    Blog Post
    Published
    Nov. 11, 2020, 2:23 p.m.
    ...any plasmid: the origin of replication/replicon. The replicon is comprised of the origin of replication ...making copies.     Types of origins of replication There are lots of origins of replication out there so...Originally published Feb 6, 2014 and last updated Nov 10, 2020. Now that we know all about antibiotic...known derivatives), pSC101, R6K, and 15A. Not all origins of replication are created equal. Some will produce...proper promoter and growth conditions. Choose your origin of replication wisely and avoid plasmid incompatibility...

  2. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type
    Blog Post
    Published
    July 2, 2020, 12:40 p.m.
    ...situ of Regulatory Elements: The original CAPTURE method The original CAPTURE method requires the creation...Originally published Dec 7, 2017 and updated Jul 2, 2020. Promoters may be the star of gene regulation...Purification in situ of Regulatory Elements) method. The original CAPTURE method was published in 2017 and addressed...by PCR or deep sequencing. A drawback of the original CAPTURE method is that a new cell line has to ... of the CAPTURE 2.0 system To improve upon the original CAPTURE method, the Xu Lab made the following ...plasmids for CAPTURE 2.0! When compared to the original CAPTURE, CAPTURE 2.0 had a ~14-fold increase in... Comparing CAPTURE and CAPTURE 2.0.     Original CAPTURE CAPTURE 2.0 Biotinylation enzyme BirA...

  3. What the HEK?

    Type
    Blog Post
    Published
    April 18, 2023, 1:15 p.m.
    ...is difficult to determine its true tissue origin. Originally, it was assumed HEKs were a common cell type...wondered where human embryonic kidney (HEK) cells originated from? Or why they are such a commonly used cell...the nomenclature we use today: HEK-293. The exact origin of the fetal tissue which HEKs were isolated from...line have determined it is most likely adrenal in origin. (Anatomy refresher: the adrenal gland is next ... them to replicate viral plasmids with the SV40 origin of replication. To generate a virus, you need only...recombinant proteins are necessary, HEKs are human in origin, so they eliminate non-human PTMs which is a concern...human neuronal cells by human adenoviruses and the origin of HEK 293 cells. FASEB J. (2002) (8):869-71. DOI...

  4. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    Published
    March 31, 2020, 1:15 p.m.
    ... the origin of replication and the replication control machinery (Need a refresher on the Origin of Replication...Replication? See our Plasmids 101: Origin of Replication blog).  The replication problem Plasmids with... directly inhibit replication by binding to the origin of replication and blocking replication machinery...problem. Iterons are repeated sequences within the origin of replication and are required for replication...machinery. However, when plasmids have the same origin of replication, the negative regulation system ...our Plasmids 101 blog posts Learn more about the origin of replication Find blog posts about plasmid cloning...

  5. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...expression levels; expression vector cannot have p15A origin of replication F- ompT lon hsdSB (rB- mB-) gal... than pLysS; expression vector cannot have p15A origin of replication F- ompT lon hsdSB (rB- mB-) gal... in E. coli. Expression vector cannot have p15A origin of replication F- ompT hsdSB(rB- mB-) gal dcm (...body formation. Expression vector cannot have p15A origin of replication fhuA2 [lon] ompT gal (λ DE3) [...pREP4 plasmid). Expression vector cannot have p15A origin of replication F-, Φ80ΔlacM15, thi, lac-, mtl-,...resistance cassette for positive selection and a p15A origin of replication, making it incompatible with other...backbones.   Our Most Popular Plasmids 101 Posts: Origin of Replication The Promoter Region – Let's Go! ...

  6. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    Published
    April 7, 2022, 1:15 p.m.
    ...look at the origins of the system and explore advances that have happened since our original 2018 blog ...This post was originally written by Tyler Ford in 2018. It was updated by guest blogger Abhi Aggarwal...blog post. The origins of iGluSnFR   iGluSnFR was developed in 2013 by Loren Looger’s lab at HHMI Janelia...through different C-terminal anchoring domains The original iGluSnFR in the pMinDisplay backbone is fused ... on the extracellular side. In addition to the original PDGFR TMD, the researchers introduce iGluSnFR3...

  7. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

    Type
    Blog Post
    Published
    April 2, 2019, 1:44 p.m.
    ...this is where most of our conference room names originated from. Let’s take a look at some of the stories... SB transposon system including those from the original publication, Ivics et al 1997. Spaghetti Squash...may think that a gene called "spaghetti squash" originated from the squash itself, but think again. Spaghetti...spaghetti? I actually couldn’t find the specific origins of the name but some of our Addgenies had an idea...Azplazu and Frasch, 1993). I could not find the exact origin of this gene name but the larval gut of bagpipe...learned some fun facts about these genes and the origins of their names!    References Azpiazu, Natalia...

  8. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    Published
    June 20, 2024, 1:15 p.m.
    ...to the same extent. As described in our previous Origin of Replication post, DNA replication is initiated...4.6 million base pairs long and contains a single origin of replication (oriC), where initiation begins..... Thanks, stringent regulation! This post was originally written by Jason Niehaus in December 2015 and...., Dyson, K., Rao, P., & Leonard, A. C. (2018). Origin recognition is the predominant role for DnaA-ATP...Han, E., Paulsson, J., & Chattoraj, D. K. (2001). Origin pairing (‘handcuffing’) as a mode of negative control...Resources at Addgene Read our blog post on the Origin of Replication Browse other Plasmids 101 Posts ...

  9. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...high-copy number bacterial ColE1 origin with the RSF1010 origin, an IncQ origin with broad host-range (Meyer...suite of plasmids (pGT) with various replicative origins that can be used for broad or narrow range of microbial...microbial species. These also contain the RP4 transfer origin as well as a selectable marker and a desired genetic...

  10. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Jan. 13, 2025, 9:49 p.m.
    ...flexibility to the CRISPR editing world. The approach, originally published in Nature, is a “search-and-replace...HDR is typically less than 10%. In contrast, the original paper introducing prime editing demonstrated ~...into one strand, there’s a mismatch between the original sequence on one strand and the edited sequence...indels by 13-fold (Anzalone et al., 2019). PE3, as originally introduced, relies on the PE2 enzyme. PE4 and...and DNA ligases to act first. PE4 and PE5, as originally introduced, rely on the PE2 enzyme. PEmax: Optimizing... plasmids from David Liu's lab! This post was originally written by Jennifer Tsang in October 2019 and...

  11. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ... the deadline isn’t going to be much use. 2. Originality is essential for grant success The two most important...be looking for in your research proposal are originality and relevance. When reading through your proposal...our own or other groups, but your work must be original (otherwise why would you be interested in it?)...Frontier Science Program's website. One way to add originality to your work is to collaborate with specialists... applications to solve problems will provide originality to your work and possibly a new perspective on...

  12. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    Published
    April 13, 2021, 1:15 p.m.
    ... of these CRISPR systems. The original pCas/pTargetF system Our original pCas/pTargetF system consists...Originally published Apr 13, 2021 and last updated Aug 12, 2021. This post was contributed by guest bloggers... to yield pEcCas. In pTargetF, we replaced the original 20 bp specific sequences on pTargetF with the ...of the pEcCas/pEcgRNA system Compared with the original system, the pEcCas/pEcgRNA is superior in several...

  13. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...history of the cell line consisting of records of the origin and propagation of the line, and can include assays...isoenzymes and genetic polymorphisms specific to their origins. This technique is still used today to test for...researchers are free of contamination and match their original description. The oldest, largest, and most diverse...This is to ensure the cells are as close to the original as possible and so any results generated are reproducible...you can retain the maximum stocks and use of the original Cell line you purchased, keeping all experiments...

  14. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    Published
    Sept. 29, 2015, 2:30 p.m.
    ...plant species. Thus, binary vectors contain two origins of replication, one each for E. coli and Agrobacterium...borders to 25 bases each. By switching the E. coli origin of replication and codon-optimizing the Agrobacterium...interest with a constitutive, robust M24 promoter. Originally isolated from the Mirabilis mosaic virus, the...Synthetic Biology, MoClo, and More Plasmids 101: Origin of Replication    ...

  15. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...domain unfolds allowing it to bind to SspB. This original Light Inducible Dimer (oLID) could be used to ...routinely reaches levels 20-40x greater than the original dCas9-VP64 activator. They also revealed that ...enzymes, such that the ligation regenerates the original restriction sites for further iterative cloning... use of electrodes. The Cohen lab has made the original Arch indicator (pJMK004), as well as the CheRiff...Nano-lantern fusions are designed to complement the original yellow variant, with all 3 having distinct emission... the high rate of read-through transcription originating from cryptic promoters in the plasmid backbone...design pXPG, the authors incorporated a high-copy origin of replication and a modified luciferase gene into...

  16. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    Published
    April 2, 2020, 6:17 p.m.
    ...Originally published Jan. 14, 2014 and updated Apr. 14, 2020. Any newcomer who joins a molecular biology...and/or virulence. All natural plasmids contain an origin of replication (which controls the host range and...another cell. Minimally, lab-created plasmids have an origin of replication, selection marker, and cloning site...described below.   Vector Element Description Origin of Replication (ORI) DNA sequence which allows ...

  17. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

    Type
    Blog Post
    Published
    Jan. 21, 2020, 2:30 p.m.
    ...accessibility of CRISPR-based phenotypic screen datasets. Original published CRISPR screen data is curated and deposited... Each screen curated in ORCS is linked to the original publication via its PubMed identifier, which can...metadata.   Screen results are reported as the original quantitative gene-level scores provided by authors...rather than sgRNA-level sequence read scores. The original supplemental data tables available with the publication...

  18. Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation

    Type
    Blog Post
    Published
    Nov. 8, 2018, 3:47 p.m.
    ... another organism in the lab. Predicting lab-of-origin of engineered DNA This isn’t the only time researchers...Addgene plasmid datasets) to predict the lab-of-origin of engineered DNA. Both of these groups have generated...biosurveillance tool,” Kunjapur says. Predicting lab-of-origin and synthetic vs. natural gene are essential and... A. Voigt. "Deep learning to predict the lab-of-origin of engineered DNA." Nature communications 9.1 (...

  19. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

    Type
    Blog Post
    Published
    June 17, 2021, 1:17 p.m.
    ...Di Ventura (@barbara_synbio) May 13, 2021 The original AraC transcriptional regulator Their system is...light-inducible dimerization domain, Vaucheria frigida aureochrome1 (VfAu1), led to functional BLADE variants...does BLADE have over AraC? When compared to the original AraC system, BLADE is simply more precise. “We...light switch and shuts off just as quickly. The original AraC system, which uses arabinose, requires many...

  20. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ... photostability and brightness compared to the original, though its maturation is even faster. Published...maintaining the bright and stable fluorescence of the original (Ivorra-Molla et al., 2023). They also named their...collection includes a mouse and a chimeric rabbit (original mouse Fab region with rabbit Fc) anti-FLAG antibody...antibodies for use in western blots, though the original parent clone (M2) has been widely used in a variety...
Showing: 1 - 20 of 262 results