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We narrowed to 8 results for: rigi

Showing: 1 - 8 of 8 results
  1. Molecular Biology Reference

    Type
    Guide
    ...Biology Reference Jump To: Molecular Biology Basics Origins of Molecular Genetics Plasmid Elements Working ...Genetic Code Webpage and Blog References Protocols Origins of Molecular Genetics The concept of genes as carriers... this purpose, which includes a DNA replication origin, an antibiotic-resistance gene, and a region in...parental cell, and are thus termed clones of the original cell. The plasmid DNA is similarly referred to...their simplest form, plasmids require a bacterial origin of replication ( ori ), an antibiotic-resistance...common plasmid elements: Plasmid Element Description Origin of Replication ( ori ) DNA sequence which directs... cultured human cells. Although plasmids were originally used to understand protein coding gene function...
  2. Sequencing Primers

    Type
    Guide
    ...reverse primer F1ori-F GTGGACTCTTGTTCCAAACTGG F1 origin, forward primer GAL1 AATATACCTCTATACTTTAACGTC (...forward primer pACYC-F TGAAGTCAGCCCCATACGAT p15A origin, forward primer pAd-CMV GCTAGAGATCTGGTACCGTC For...vector pBR322ori-F GGGAAACGCCTGGTATCTTT pBRS322 origin, forward primer pBRforBam CTTGGAGCCACTATCGAC In... primer pGP704-R AACAAGCCAGGGATGTAACG R6K gamma origin, 3' of MCS in pGP704 vector, reverse primer pHybLex...primer SV40pro-F TATTTATGCAGAGGCCGAGG SV40 promoter/origin, forward primer SV40-spliceR CACAAAGATCCGGACCAAAG...
  3. Modular Cloning Guide

    Type
    Guide
    ... K. phaffii ). GoldenPiCS Kit Yeast Expression Brigitte Gasser , Diethard Mattanovich , and Michael Sauer... and Cultivarium Tools 189 plasmids, including origins of replication and selection markers for a variety...Kit Genome Engineering, CRISPR, Yeast Expression Brigitte Gasser Plasmids to perform CRISPR/Cas9 mediated...
  4. CRISPR Guide

    Type
    Guide
    ...target sequence present in the gRNA. CRISPR was originally employed to knock out target genes in various..., be sure to check the library information and original publication to see if a library is suitable for... of your amplified library matches that of the original library. Next, you’ll need to generate lentivirus...
  5. Antibody Guide

    Type
    Guide
    ... and can either be used while suspended in the original media or isolated and placed in a buffer. Antibody...antibodies can distinguish between different species of origin, isotypes, and isotype subclasses of a primary ...
  6. Cloning

    Type
    Guide
    ...any plasmid backbone independent of how it was originally designed to be cloned into. A drawback of this...
  7. Lentiviral Vector Guide

    Type
    Guide
    ...Braun, S. E. (2021). HIV-based lentiviral vectors: Origin and sequence differences. Molecular Therapy — Methods...
Showing: 1 - 8 of 8 results