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We narrowed to 512 results for: sam

Showing: 181 - 200 of 512 results

  1. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    Published
    Nov. 3, 2015, 3:30 p.m.
    ...that the CRISPR system was involved. Today, that same laboratory enjoys a successful edit rate of over...later it became quite clear that others thought the same. Since January 2013 there have been 33 CRISPR software...knowledge from laboratories employing CRISPR on the same organism could be used as a proxy for this last ...

  2. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...function like your average AAVs — and have many of the same restrictions, like limited cargo space (~4.7 kb)...systems, which use two or more AAVs to transduce the same cell, can accommodate larger, modular genetic payloads...efficient AAV-PHP.eB, developed and deposited by the same lab. AAV-PHP.eB is the most commonly requested systemic...off-target organs. AAV.CAP-B22 was developed through the same process as AAV.CAP-B10 and shows high transduction...

  3. Addgene’s Blog Gets a New Look!

    Type
    Blog Post
    Published
    Jan. 25, 2021, 5:54 p.m.
    ...Say hello to the new Addgene blog! Same URL, same great articles to help you with your experiments, but...

  4. AAV Packaged on Request is Here!

    Type
    Blog Post
    Published
    Jan. 10, 2025, 1:01 p.m.
    ...plasmids and your viral packaging service in the same place, making it easier to go from selection to ...control.  Our packaged on request AAV service uses the same trusted production and quality control processes...

  6. 6 Tips for Analyzing and Troubleshooting Sanger Sequencing Results

    Type
    Blog Post
    Published
    June 26, 2014, 2:23 p.m.
    ...less and you might suspect contamination in your sample or consider asking your sequencing facility to ...Use a silica spin column for purification of the samples you send for DNA sequencing If your sequencing ...

  7. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

    Type
    Blog Post
    Published
    Jan. 23, 2014, 3:23 p.m.
    ...toolkit - aptly named GreenGate – offers all of those same advantages to researchers working in plant model...allows us to design modules that always have the same recognition site but distinct sites where the enzyme...

  8. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    Published
    June 19, 2015, 3:08 p.m.
    ...N- and C-terminal. Each set of plasmids uses the same restriction enzymes for cloning (see schematic below...one need to remove the tag post-purification. A sample of pCri constructs: pCri-1a, -4a and -9a. 6xHis...

  9. Science as Art

    Type
    Blog Post
    Published
    March 2, 2015, 3:13 p.m.
    ...could provide a new tool to study old and unique samples, such as those collected by Darwin on his voyage...incompatible with the need to conserve these unique samples, meaning that we know very little about these specimens...

  10. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    Published
    Oct. 15, 2024, 1:15 p.m.
    ...produce your Packaged on Request prep and run the same quality control assays we run with all of our in-stock... be 1x1013 and titers for AAV2 to be 7x1012, the same titers found in our in-stock preps. If a request...

  11. A Better Way to Get Customer Support: The Help Center

    Type
    Blog Post
    Published
    Dec. 10, 2015, 3:30 p.m.
    ...resources with our new Help Center! Created using the same reliable Zendesk system that powers our email support...support team’s attention, where we can then offer the same high level of direct service you’ve come to expect...

  12. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    Published
    June 11, 2020, 1:34 p.m.
    ...optogenetics in a mouse experiment. Viral delivery is the same in both cases, but activation depends on small molecule...repolarize faster.  Chemogenetics does not offer the same level of control over timing. Because chemogenetics...

  14. Bricking Science: Portraying Scientific Reality Through LEGO

    Type
    Blog Post
    Published
    Nov. 29, 2016, 3:30 p.m.
    ...wrong. Whether it be a bad fridge freezing your samples, or a dysregulated water bath boiling your experiments...researcher doing some experiments in Brazil can have the same issues as one doing experiments in France. We can...

  15. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    Published
    Dec. 9, 2014, 2:22 p.m.
    ...that it takes to be a good scientist are not the same ones it takes to be a competent manager (much less...job satisfaction Being a good manager is not the same as not being a bad manager (see cartoon above – ...

  16. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    Published
    May 9, 2019, 1:15 p.m.
    ...phenotype typically emerges. Every three days, a sample of cells was collected for scRNA-seq analysis and...et al. "CellTag Indexing: genetic barcode-based sample multiplexing for single-cell genomics." bioRxiv...

  18. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type
    Blog Post
    Published
    Sept. 17, 2020, 1:15 p.m.
    ... over.” And we sent the Addgenies home.  At the same time, as Addgene started dealing with this new reality...and keep new projects on track to succeed at the same time. I began approaching science funding organizations...

  19. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    Published
    Oct. 26, 2017, 1:59 p.m.
    ...point of reference, Addgene commonly prepares NGS samples using both pre- and post-amplification DNA and ...libraries, but any analysis script should return the same output: a spreadsheet of read counts. You can use...

  20. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    Published
    Sept. 6, 2017, 1:01 p.m.
    ...fragments containing the desired mutations. This same process can be used simultaneously for the generation...cassette, ready for transformation into yeast that same day.  The field of synthetic biology has led to ...
Showing: 181 - 200 of 512 results