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Addgene

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We narrowed to 599 results for: REV;

Showing: 101 - 110 of 599 results

  1. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ...Saccharomyces cerevisiae. Paques F and Haber JE. Microbiology and Molecular Biology Reviews (1999). PubMed...repair and genome engineering CRISPR-Cas9 has revolutionized the genome engineering world and made targeted... disrupting one or both of these sequences. To prevent re-cutting and the resulting mutations after an...transcribes RNA encoding your repair template, then uses reverse transcriptase to generate the complementary ssDNA...

  2. Transferable Skills Guide: Time Management

    Type
    Blog Post
    Published
    Dec. 5, 2017, 1:52 p.m.
    ...s post and give it a final read Browse list of previous depositors from Boston University Contact Dr. ...that post from Eric before doing anything else. Preventing procrastination Even if you’ve done all the prioritizing...planning in the world, procrastination can easily prevent you from making progress on a task. For instance...to go sit in the tissue culture hood may have prevented me from splitting cells until late in the day ...podcasts. JUST GET STARTED - One Harvard Business Review article on procrastination recommends testing out...

  3. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    Published
    Nov. 25, 2015, 4 p.m.
    ...lack of complete information does not, however, prevent us from identifying problems with the structure...take on opportunities to do some blogging, write reviews, or write for a university publication, improving...was on diversity in academic research. As during previous panels, speakers Moon Duchin, Rafael Luna, Joan... need some way of capturing, quantifying, and preventing this type of failure. Final thoughts The FOR ...Additional Resources The Postdoctoral Experience Revisited. PubMed PMID: 25590106. Paula Stephan’s Book -...

  4. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ...efficiency of 30-60%. This is much more favorable than previous methods yielding 1-10% knock-in. Read on to learn...transcription, and use the IVT product as a template for reverse transcription to create your ssDNA. Note that the...conditional deletion of exon 6 of Syt1, mimicking a previously made mouse model. To avoid disrupting splice ...separates the gRNA target sequences from their PAMs, preventing re-cutting after editing. How to use Addgene ...Additional Resources on the Addgene Blog Read Our Previous Post on CRISPR Mouse Models Learn about Homology...

  5. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ... the University of California, San Diego. The revolution in genetic engineering techniques is a speculation...and just when the Mendelian genetics was being revived by the botanist Hugo de Vries (1896).  In 1928,...Blade Runner (1982). It is a dystopian film that revolves around synthetic humans called replicants which..., and C. In this movie Ethan Hawke’s character revolts against class divide that is created by genetics...prime editor which is a fusion between Cas9 and reverse transcriptase. Using an elongated template + guide...

  6. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...transfer worked with Ponceau S staining or other reversible protein staining immediately after your transfer...expecting low amounts of protein, make sure your reversible staining method of choice is appropriately sensitive...transfer buffers. To slow down smaller proteins and prevent them from over-transferring, increase the amount... away the SDS. To speed up larger proteins and prevent them from under-transferring, try increasing the...Technical errors Search online/ask an expert Review protocol videos for a visual guide to good technique...Check transfer with Ponceau red stain or other reversible stain* Roll out all of the bubbles in the...

  7. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    Published
    Aug. 15, 2017, 1:24 p.m.
    ...real time. Two-photon microscopy was the next revolution in cell imaging, giving researchers the ability...(intravital imaging) studies it’s enabled are revealing the cellular behaviors that mediate adaptive and...proteins (protein that undergoes light-induced irreversible photoconversion from green fluorescence to red... 1. Peters, Nathan C., et al. "In vivo imaging reveals an essential role for neutrophils in leishmaniasis.... Egen, Jackson G., et al. "Intravital imaging reveals limited antigen presentation and T cell effector...

  8. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    Published
    March 14, 2017, 2:30 p.m.
    ...immune cells harvested from patients and thereby prevent cancer cells from using this receptor to evade ...immunotherapies currently under study. Check out this review for an in-depth look at a variety of up and coming...monoclonal antibody-based therapeutics." Nature Reviews Cancer 15.6 (2015): 361-370. PubMed PMID: 25998715...Lloyd J. Old. "Antibody therapy of cancer." Nature Reviews Cancer 12.4 (2012): 278-287. PubMed PMID: 22437872...Cancer immunotherapy via dendritic cells." Nature Reviews Cancer 12.4 (2012): 265-277. PubMed PMID: 22437871...

  9. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    Published
    Sept. 10, 2015, 2:30 p.m.
    ...optogenetics. Annual review of neuroscience. 2011;34:389-412. doi: 10.1146/annurev-neuro-061010-113817....effectiveness of your optogenetic manipulations (though a reviewer will probably ask for electrophysiology data too...best suit your specific experiment. Consult these reviews for a discussion of the different variants [1, ...underlying brain disease in animal models. Nature reviews Neuroscience. 2012;13(4):251-66. doi: 10.1038/nrn3171...

  10. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    Published
    Nov. 19, 2013, 2:37 p.m.
    ...integrate protein tags into the genomes of S. cerevisiae and S. pombe. The protocol is surprisingly simple... in yeast. These tagging vectors are based on previously described pFA6a-link vectors and include a Kan...assessed many of these fluorescent tags in S. cerevisiae, looking at their performance in categories such...a complimentary set of plasmids for use in S. cerevisiae, with the additional benefit of multiple selection...
Showing: 101 - 110 of 599 results