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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 576 results for: BASE;

Showing: 401 - 440 of 576 results

  4. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    Published
    Aug. 19, 2014, 3:39 p.m.
    ...Fly vectors The vast majority of fly vectors are based on p-element, random or targeted, integration into...

  5. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    Published
    Jan. 30, 2014, 3:29 p.m.
    ...synthetic compounds. Antibiotics can be categorized based on whether they directly kill bacteria (bactericidal...

  6. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    Published
    Oct. 24, 2017, 1:54 p.m.
    ...against unnecessary clinic regulations, evidence-based research is vital in combating misinformation and...83.7430°W – Alexandra Greenbaum Science and evidence-based policy and legislation hit home hard in Michigan...

  7. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    Published
    March 7, 2017, 3:30 p.m.
    ... a single allele in a human line, using methods based on homologous recombination (Rago et al., 2007)....opportunities, it’s an exciting time to be in the lab. Based on the power of available techniques and how quick...nuclease cleaves double stranded DNA (dsDNA) at basepair mismatches. To use this tool to detect a BRAF ...strand from the control cells, which should contain basepair mismatches at the site of the edit. Using Surveyor...

  8. An Addgene Summer Internship

    Type
    Blog Post
    Published
    Aug. 8, 2024, 7:22 p.m.
    ...expected something wrong. My capstone project was based on plasmid cloning and I, the researcher, got to...

  9. The MTA at Addgene

    Type
    Blog Post
    Published
    July 23, 2024, 2:17 p.m.
    ...be distributed for research use only to industry-based scientists. Some organizations choose to share their...

  12. Plasmids 101: Origin of Replication

    Type
    Blog Post
    Published
    Nov. 11, 2020, 2:23 p.m.
    ...generally high in As and Ts. Why, you ask? Well, A-T base pairs are held together with two hydrogen bonds ...

  14. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    Published
    April 25, 2023, 1:15 p.m.
    ... antibodies. Recombinant antibodies are plasmid-based and are created synthetically or derived from hybridoma...

  28. Components of CRISPR/Cas9

    Type
    Blog Post
    Published
    Feb. 2, 2016, 5 p.m.
    ... sequence contained within the guide RNA and a 3-base pair sequence known as the Protospacer Adjacent ...

  29. Finding and Joining Your Dream Lab

    Type
    Blog Post
    Published
    April 8, 2014, 2:50 p.m.
    ...interests.” George Church selects new hires initially based on those who notice and embrace the “odd and extreme...

  30. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ... Knockout cell line approach Genetic knockout-based validation can conclusively prove the ability of... through its ability to separate the cell lines based on expression of the target. In this case, you could...

  32. OCaMP: A New Calcium Indicator for Neural Imaging

    Type
    Blog Post
    Published
    Aug. 19, 2025, 1:15 p.m.
    ...calcium indicator, OCaMP (Aggarwal et al., 2025), based on the fluorescent protein mOrange2 (Figure 2). ...scaffold of OCaMP) are shaded in orange. Data from FPbase.org. Scientists have developed calcium indicators...

  38. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ...Blast Most of Addgene’s lentiviral inventory is based on CRISPR tools, though we do provide a few non-...
Showing: 401 - 440 of 576 results