We narrowed to 583 results for: BASE;

..., etc). The cloning method is ultimately chosen based on the plasmid you want to clone into. Regardless...

... genetically-encoded voltage indicators (GEVIs) based on the ASAP sensors. The resultant JEDI-2P sensors...

...audience can understand the conflict. A science-based conflict gives you an easy framework with which ...

...Transformation CRISPR 101: CRISPR-Mediated Plant Base Editors ...

...Kagiampaki et al., 2023. nLight nLight sensors are based on alpha-1a adrenergic receptor; nLightG contains...

...write a few blog posts. This first post will be based on recycling! With the popularity of single-use ...

...repair (HDR) are echoed in the amount of interest in base editors, which alter genomic DNA without the need...

...about six months, I learned there was a Genzyme-based running team called Running for Rare Disease that...

...will receive much better health care in the future based on our studies.” Thank you to all the BeHeard Awardees...

...Douglas K. Smith or the book on which this article is based.          ...

...Fly vectors The vast majority of fly vectors are based on p-element, random or targeted, integration into...

...synthetic compounds. Antibiotics can be categorized based on whether they directly kill bacteria (bactericidal...

...against unnecessary clinic regulations, evidence-based research is vital in combating misinformation and...83.7430°W – Alexandra Greenbaum Science and evidence-based policy and legislation hit home hard in Michigan...

... a single allele in a human line, using methods based on homologous recombination (Rago et al., 2007)....opportunities, it’s an exciting time to be in the lab. Based on the power of available techniques and how quick...nuclease cleaves double stranded DNA (dsDNA) at basepair mismatches. To use this tool to detect a BRAF ...strand from the control cells, which should contain basepair mismatches at the site of the edit. Using Surveyor...

...expected something wrong. My capstone project was based on plasmid cloning and I, the researcher, got to...

...be distributed for research use only to industry-based scientists. Some organizations choose to share their...

...the authors of these papers did. Since the SunTag-based system tethers mRNAs to the plasma membrane, it’...

...Include follow-up questions or requests for advice based on your discussion. Perhaps you have a paper, protocol...

...generally high in As and Ts. Why, you ask? Well, A-T base pairs are held together with two hydrogen bonds ...

... antibodies. Recombinant antibodies are plasmid-based and are created synthetically or derived from hybridoma...

...calcineurin. Current calcineurin gene reporters are based on the bacterial βgal assay, and are not suited ...

...and how components of the plasmid should change based on bacteria type. The gram positive and negative...

...Fink, Y., & Anikeeva, P. (2019). Flexible fiber-based optoelectronics for neural interfaces. Chemical ...

.... I am the cofounder and CEO of miLab, a Boston-based startup focused on empowering early-stage scientists...

...types and subtypes using polythetic classification based on evolutionary similarity (5). Type II CRISPR/Cas...

...quickly learned that my approach needed to adapt based on individual needs. For instance, I had one colleague...

...activation of transcription in bacteria by co-opting σ54-based transcription. Transcription from σ54 promoters ...

...application for OptoNBs takes us into cells: light-based control of signaling pathways. This is particularly...

...expression.     AAV retrograde Unlike traditional dye-based neuronal tracing reagents, AAV particles require...

...consistent with the cell type that was predicted based on the Atlas. They also looked at 17 of these TFs...

...Found in surgical suites, air fields, and military bases, checklists are the ultimate tool for doing things...

...0460.1000439 6. Wu et al. 2020. . “Advances in CRISPR/Cas-based Gene Therapy in Human Genetic Diseases.” Theranostics...

...3 nonprofit and our plasmid service was started based on donations. That seed money was used to build ...

... sequence contained within the guide RNA and a 3-base pair sequence known as the Protospacer Adjacent ...

...interests.” George Church selects new hires initially based on those who notice and embrace the “odd and extreme...

... Knockout cell line approach Genetic knockout-based validation can conclusively prove the ability of... through its ability to separate the cell lines based on expression of the target. In this case, you could...

...liability in respect to actions taken or not taken based on any or all the contents of this post.  An attorney...September 2017, USCIS received 116,224 employment based green card applications and approved 91,023 during...

...gene delivery to mammalian cells using 293T cell-based systems. Curr Protoc Immunol. 2001 May;Chapter 10...

...specificity. This can be especially important for base editing techniques, where Cas9 must be directed ...

...screens of immune cell activity. However, a CRISPR-based tool for in vivo functional analysis of immune cells...