... to the immobilised target sequences. Upon binding, changes in fluorescence can be visualised. Another...

...in eukaryotic cells as an intermediate of heme metabolism (Shemetov et al, 2017). Modern state-of-the-art...protein expression level. State-of-the-art FPs (listed in Table 1) were optimized for efficient binding...

... research is about and what you think you can accomplish. No matter how many times I say this, candidates.... One of my graduate students encouraged me to publish this manual and earn some cash for my expensive...

...valuable tool to study cellular physiology and metabolism under various conditions. LiLac has improved ...capable of local temperature sensing with sub-millisecond resolution and sub-degree temperature differences...

...especially if the options outlined above aren’t realistic. Check Out Our Post on Sequencing Options for ...like CRISPRitz or CRISPOR can help narrow down the list of sites where off target editing may occur, allowing...

..., scientists engineered a mutant BirA* from B. subtilis, termed BASU. BASU exhibits 1,000 fold faster ...molecules per cell volume? A call to rethink some published values." Bioessays 35.12 (2013): 1050-1055. PubMed...

...used to purify proteins from E. coli, Bacillus subtilis, or Pichia pastoris depending upon your specific...Miller, Corwin, et al. "Adaptation of Enterococcus faecalis to daptomycin reveals an ordered progression to...

... Twitter. Be ready for the interview with a long list of written questions for the PI and lab members....too, they may not have had experience writing, publishing, or discussing career opportunities with the ...

....    Flow cytometry In addition to the controls listed above, viability and compensation controls are ...antibody-based experiments, it is by no means an exhaustive list and we encourage you to review the literature for...

...the right information When using a previously published system in your own work, you’ll need to know (...Bamberg, E., Nagel, G., & Deisseroth, K. (2005). Millisecond-timescale, genetically targeted optical control...

...levels, most of them will also give you a specific list of the candidate off-target sites. For example, ...given threshold even when you’ve tried everything listed here. For some applications, this is ok, but it...

.... The plasmids used to construct these RSMs are listed in the table below and can be requested through...sequences on their 5’ and 3’ ends (for insulation). A list of promoters and terminators used in our GRSM registers...

...duration of only three months and made a good job to list on my CV. Three months was a perfect duration for...reason why I chose Australia was because it is an English speaking country with a strong economy and plenty...

... generations of lentiviral vectors have been established so far, with safety increasing in each generation...methods are broadly used for virus titration: the p24 ELISA dosing method detects the expression of viral capsid...

...Originally published Jan 27, 2015 and last updated Dec 17, 2020. This post was contributed by Jordan ...experimentation. In 2014, several manuscripts published in Genetics detailed approaches in which selection...

...for obtaining a material transfer agreement, and list of vendors (such as the vector core at the University...the many primary research papers that have been published at this point.  In brief, some standard opsins...

...manufacturer and/or in the published literature. Note that the antibody being published doesn’t always indicate...

...sequencing or other readout. Cellular memory is accomplished by the use of sequence-specific enzymes (e.g...also available, N1-LOC8R.  These plasmids are unpublished Unnatural amino acid incorporation in mammalian...developed rapid-response luciferase (firefly P. pyralis) reporter plasmids for use in high-throughput screening...

...validation assays. If you’re using previously published antibodies, check the manuscript and supplementary...weight of the proteins you’re interested in. Table 1 lists the most common types of gels for SDS-PAGE running...

...shows how antibodies are used to study proteins via ELISAs, western blots, immunoprecipitation, immunohistochemistry...

...processing of our sequencing data.”  Recently, Seqera published a case study on how Addgene has used Nextflow ...

... the sharing of reagents and data, we’re also publishing research. Addgene’s research team also investigates...

... away. Is your favorite science comic not on our list? Let us know by sharing it with us in the comments...

...University of Western Australia.    🤩 our lab in Bayliss Building @SMS_UWA is getting festive 🎄🎁🎅🤶🎄...

...journey into synthetic biology. The guide is a curated list of research papers, blogs, free courses, YouTube...

... Dr. Baker’s team was the first to develop and publish a human-designed protein that was not based on ...

...screenshot above). Don’t see your favorite organism listed? Help us serve you! Let us know about other organisms...

...request plasmids, but I also spend a lot of time listening. I’ve had hundreds of conversations with PIs, ...

...of the viral preps. For example, last year we published an article describing a sucrose supplementation...

...all, and to all a good light!    🤩 our lab in Bayliss Building @SMS_UWA is getting festive 🎄🎁🎅🤶🎄...

..., 2022.  The Addgenies will choose up to four finalists, and the final winner will be determined by popular...

...decorating skills. To join the contest, simply embellish and adorn your lab space or your work from home...

...Originally published May 31, 2018 and last updated Jan 27, 2021 by Jennifer Tsang. We have some crisp...

...podcast all about graduate student life and how establishing a thriving graduate student community can make...

...plasmids from the collection. You can find the full list here. Most of these plasmids are empty backbones...

...explore other areas such as scientific sharing or publishing, under the Other dropdown menu. You can also ...

...given a DOI for easy citation by others and, when published, will meet FAIR data-sharing guidelines. But ...

...interest to you, our readers. Below I’ve compiled a list of the Top 10 Most Viewed Posts on our blog from...

... available. I recommend a few: -The recently established Ebola Genome Portal at the University of California...

...success.  In conclusion, it is important for you to establish in advance how you want to utilize your plasmid...