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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 573 results for: BASE

Showing: 501 - 550 of 573 results

  1. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    Published
    Jan. 23, 2020, 2:45 p.m.
    ...development called Scrum. The Scrum framework is based on an iterative and collaborative approach to development...

  2. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ...particularly important if your amplicon is longer than 150 base pairs. While we use ddPCR for AAV titration, there...

  9. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ... neuron can then be traced throughout the brain based on its unique hue (plasmids at Addgene). A large-scale...Its efficiency is comparable to conventional dye-based tracing while also having the added advantage of...

  10. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    Published
    Aug. 20, 2020, 2:30 p.m.
    ...genomic edit. Some Cas13 enzymes require a single base protospacer flanking sequence (PFS) adjacent to ...from Staphylococcus aureus (SaCas9) is about 1 kilobase smaller than SpCas9, so it can be packaged into...

  12. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ... in the envelope and are targets for complement-based killing by anti-alphaGal antibodies. The VSV-G envelope...

  15. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...should first develop primers to the approximately 500 base pair sequence surrounding your gRNA. Once amplified...

  17. March for Science

    Type
    Blog Post
    Published
    April 21, 2017, 2:30 p.m.
    ... the meaning of the word ‘fact’. Policy must be based on our most accurate interpretation of the world...

  19. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ...fail to recapitulate the true steric and charge-based nature of the phosphoryl-modification; they are ...appreciated. PhosphoSitePlus, one of the most inclusive databases of post-translational modifications, identifies...

  20. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ...Genome-wide programmable transcriptional memory by CRISPR-based epigenome editing. Cell, 184(9), 2503–2519.e17. ...

  21. Finding Your Science Policy Path

    Type
    Blog Post
    Published
    Sept. 22, 2020, 1:15 p.m.
    ...Think a university system who develops evidence-based university policy to combat sexual harassment in...

  22. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ...same conditions. Example: You want to sort cells based on the expression of a new receptor that is of interest...attempts by other means, such as through antibody databases like Antibodypedia or through your antibody provider...

  25. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...deficiency independently increase the frequency of base substitution in human cells. J Biol Chem. 2011 Mar...

  26. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...electrical activity to gene expression, and even CRISPR based gene editing. But, how do you decide which tools...

  27. Finding Your Perfect Job After University

    Type
    Blog Post
    Published
    Jan. 12, 2016, 3:30 p.m.
    ...Addgene’s main offices are in Cambridge MA, while I’m based in London) does bring its own challenges. I’ve had...

  30. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...chromosomal gene modification with CRISPR/cas9 and PCR-based homologous recombination donors in cultured Drosophila... recovery of knock-ins, but did result in 1-2 kilobases of additional sequence being introduced. Cre-mediated...repair templates (ie. PCR-derived dsDNA) with 30-60 basepair homology arms to knock in large epitopes, such...

  32. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ... to multiplex qPCR when using fluorescent probe-based chemistry - theoretically, up to five or six reactions...

  33. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...neutralized by pre-existing antibodies against AAV9-based vectors (and vice versa), enabling repeat dosing...

  34. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    Published
    Jan. 25, 2024, 2:15 p.m.
    ... CasPEDIA is meant to be a constantly updating database which serves as a resource for the scientific ...properties (Makarova et al). CasPEDIA is an evolving database, and all researchers are invited to contribute..., Bellieny-Rabelo, D., et al. (2023). CasPEDIA Database: A Functional Classification System for Class ...

  35. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    Published
    June 30, 2016, 2:30 p.m.
    ...exhaustive, so you may want to consult the REBASE database for more detailed information. Enzyme Dam...regulating genome replication, repairing mismatched basepairs or small indels that occur during DNA synthesis...

  36. Developing Lab Management Software for Biology

    Type
    Blog Post
    Published
    Aug. 25, 2016, 2:30 p.m.
    ..., it should have a corresponding entry in your database. Enjoy your happy lab staff. Barcoded materials...even be worth it to have extra columns in your database to the effect of `dev_name` and `science_name`... database...

  37. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...cell lines on public databases. Image attributions (from top to bottom) Database Center for Life Science... Cell Line Authentication Committee includes a database listing all cross contaminated or misidentified...

  39. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    Published
    June 29, 2017, 1:06 p.m.
    ... the locations where the enzymes will cut (in basepairs), and the total number of times they cut. Use ...nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence...

  40. Will You Be My Mentor? Finding and Asking for Mentoring Support

    Type
    Blog Post
    Published
    Feb. 4, 2014, 3:50 p.m.
    ... mentoring at Mentornet.net. This is an online database of mentors you can tap for email discussions. ...who enter their information in these searchable databases are usually offering their contact information...

  41. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    Published
    Dec. 2, 2014, 7:23 p.m.
    ...New York University School of Medicine), Europe (Basel Institute for Immunology), and Japan (University...different places have very different cultures. In Basel, I felt that the time flows much slower, like the...

  45. Reflections and Looking Ahead

    Type
    Blog Post
    Published
    Feb. 25, 2025, 2:15 p.m.
    ... unlock the potential of our extensive plasmid database — because we believe that innovation flourishes...

  50. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    Published
    Sept. 14, 2017, 12:40 p.m.
    ...diversity via the Integral Microbial Genomes (IMG) database, Harrington et al discovered a Cas9 protein from...
Showing: 501 - 550 of 573 results