...only two options, but very little data on which might work in your application. It can be quite difficult...materials and methods section. This section will vary slightly depending on the application — flow cytometry,...Antibodies 101: Validation Antibodies 101: Selecting the Right Antibody...

...In this quarterly blog series, we’ll highlight a few of the new CRISPR plasmids available at Addgene....recognizes a simple dinucleotide PAM (N4CC), lending to high target site density (average every 8 bp). Nme2Cas9...

...look and feel of addgene.org. Some you’ll notice right away, while others may be less obvious — but every...you can use the links under the search box to go straight to your materials of interest!   Figure 1: The...bar New menu Most common activities can be done straight from the new search bar, but the menu just above...

... also selected for other key properties, notably high solubility, limited affinity for other proteins,...interactions, particularly in single-molecule or high-throughput applications. Perhaps IT5 and IT6 will...Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian...

... (top right corner of this post) is an example of a seemingly clean DNA sequence (no Ns in sight). If ...clean reliable DNA sequence Anything less and you might suspect contamination in your sample or consider...

...physicists, chemists, and mathematicians. We hope to highlight every science field as we expand and encourage... This would help you direct your content to the right audience. Avoid using scientific jargons. Someone...

...In this quarterly blog series, we’ll highlight a few of the new CRISPR plasmids available at Addgene....identified 67 genera of Proteobacteria that this toolkit might be used in. Read the paper in ACS Synthetic Biology...

...pleasure to announce that we once again reached new heights on the Addgene blog: we surpassed 60,000 views ...researcher at Sanofi Pharmaceuticals. This post highlights a rare disease, Fibrodysplasia ossificans progressiva...

...science accessible in one way or another to those who might be in a position to build on that work. That’s how...to consider where the open access (OA) movement might be going is to consider some of the places it’s ...providers and participation from many well-respected and high-profile universities. We particularly recommend ...

...functionally validating the thousands of mutations that might play a role in the progression of cancer are often...mask weaker ones. Thus, the need for a faster and higher-throughput in vitro approach. Using data from The..., they generated lentiviral constructs using the High-Throughput Mutagenesis and Molecular Barcoding (...

...directory is crucial because it's hard to find the right phage for the job. Phages are viruses that can infect... have someone that really needs it, but then it might to be too late,” Sacher says. As the labs in the...sample is limited, only the labs that have the highest potential to possess the correct phages will test...

...excitation at 488 nm, photoconverted with 405 nm light, and then captured in the red state after excitation...photoconverted, so this technique may not be appropriate for high-pH cellular compartments. Sattarzadeh et al. have...Murphy CS, Hazelwood KL, Davidson MW, Looger LL. A bright and photostable photoconvertible fluorescent protein...

...In this quarterly blog series, we’ll highlight a few of the new CRISPR plasmids available at Addgene....and human cells. Using the structure of CasX at eight different states of assembly from cryo-electron ...

...within the scientific community The team behind Crowdfight COVID-19 strives to match up scientists across...with different technical knowledge that could help fight COVID-19. They’ve also collated a list of useful...  "We realized that there is now a huge pool of highly skilled scientists, willing to volunteer their ...

...In this quarterly blog series, we’ll highlight a few of the new CRISPR plasmids available at Addgene....that controls nuclear localization (LINuS). Blue light renders the NLS accessible and the complex enters...

...research and expanded choice when it comes to ordering high-quality materials from the Addgene repository. Read... be aware that this ordering process will look slightly different from our standard process. We’ll walk... and flexibility; while ensuring we can maintain high standards for both our depositors and requestors...

...on addgene.org. Additionally we also wanted to highlight our blog, daily updates, and offer better exploration...mostly see a friendly smile, but occasionally you might notice Blugene looking sad, especially if an experiment...

...efficient capture. We wanted to be able to build a highly multivalent assembly connected by covalent bonds...St. Andrews discovered the domain. It is small, highly stable and has this amazing chemistry locking the... the variation in cancer, not just the CTCs with high marker expression level. Labs have been trying different...

... has been the go-to place. In 2012, his National High Magnetic Field Lab worked with an impressive 1,350... Proteins 101: GFP Fusion Proteins - Making the Right Connection.  mEmerald plasmids and many more! Baird...know how all the parts go together, we can fix it. Right now, we have no idea." Now, it will be that much...

...Cas9, then with the sgRNAs, and can result in a higher titer due to the smaller size of the plasmids. ...of selection. Shalem and Sanjana say they kept a high representation of library within cells and aimed... construct, but in some biological systems one “might need larger numbers.” Further details and protocols...

...endophyte screening system and plans to employ the light‐inducible bacterial expression system pDawn to clone... India Dr. Mrinalini Nair, Associate Professor (Right), focuses on molecular biotechnology for improving... Rivera Solís, Associate Professor (above, far right), thrives to make affordable biosensors that detect...

... LEGO and science: The potential is endless One night, while opening a LEGO set with my husband, I thought...that resembles a real laboratory. It only took one night to build the first model, but because we are [email protected]. Who knows, your story might just get “legofied.” Many thanks to our guest blogger...

... available, and have deep tissue penetration and high spatial resolution. By piecing together genes from...are sequentially exposed to acoustic pulses with high enough amplitudes to first collapse arg2 and then...development of ARGs and have already developed a high-throughput screening method for serial acoustic ...

... these results, it’s clear that editing is much higher in the PAM-out configuration. In addition, D10A...D10A editing efficiency is consistently higher than that of H840A, especially with smaller nick distances...cuts close to your insertion site, you can’t obtain high HDR efficiency. Nickases create a staggered cut ...

... Hill. Chromatin immunoprecipitation followed by high-throughput sequencing, ChIP-Seq, is the go-to method... purified for subsequent library preparation and high-throughput sequencing. Improvements to the CUT&RUN...challenging to collect large numbers of cells or when a high degree of signal to noise is desired. It also provides...

...communicated the science in my blog posts. This post highlights several of these free tools which will hopefully... selecting “Tools” and then clicking the “Usage rights” dropdown menu, you can search for images “Labeled...

... in signaling processes including smell, taste, sight, inflammation, and neurotransmission. But with approximately...activated downstream of GPCRs that are not involved in sight (Olsen RHJ et al., 2020). A transducer complex consists...Gγ-GFP2 will be in close association, resulting in a high level of GFP2 emission. Following activation in ...

...protocol, you might have noticed that there are many options to elute your DNA prep. You might see protocols...denature, break, and even change the DNA sequence. High pH favors the breakage of the hydrogen bonds, destabilizing...

...knowledge base extensively, you open doors to fresh insights and broaden your understanding, ultimately enhancing...areas in need of improvement. These unrelated ideas might hold the key to unlocking new perspectives or even...to leave a little space for the unexpected — it might just be the key to unlocking the next frontier of...

...Chemistry symposium and a career night. The society also visits high schools to promote chemistry degrees...scientific meetings for students to attend. It also hosts high level academic debates for its members to become...

...arrive. Getting off the plane in Bozeman Sunday night, it was easy to spot meeting participants. They ...the ones holding poster tubes (or as our baffled flight attendant called them, "long, skinny things") and...thinking I was an engineer. The talks we heard Monday night represented engineering first and foremost, from...

... allow his team to work mostly at home. Having a high degree of freedom and diversity built into their...new things, diversity of responsibilities, which might include training or teaching others, research, and...sometimes taken to an extreme by scientists) Are highly mobile and can move to a new workplace if opportunities...

...telling it another way. You’ll know you are on the right track if they start asking you questions. Remember...Escape from the Ivory Tower.”  9)   If you think you might want to get serious about science writing, look ...News. Ask your institutional news office if there might be opportunities to blog or write for them. 10)  ...

...bacteriostatic reagents can kill bacteria when used at high concentrations (and vice versa). Looking around ...Antibiotics vary in their sensitivitly to heat and/or light – do not add them to media hotter than about 55oC...55oC and store plates/stocks wrapped in foil if a light-sensitive antibiotic like Tetracycline is used. ...

...surprisingly more rigorous in industrial settings Tight (sometimes impossible) timelines—others are usually... <- Can move between the two (helps to know the right people) -> How can you make a decision about ...interests and passions is another great way to shed light on what paths to pursue. Spend some time really ...

...’d like to show our gratitude on the blog by highlighting some of the great posts written by Addgene depositors...to activate neurons and neuronal pathways using light and chemicals respectively. Didem Goz Aytruk from...

...Delivery The RNP delivery method consistently leads to high on-target editing and low off-target editing rates...SpCas9 The closer your edit is to the PAM site, the higher the efficiency of HDR repair. Check out this post...post to see if an engineered Cas9 variant might work for your experiment. PITChing MMEJ as an Alternative...

...scientists, MTAs are important—they guarantee certain rights and protections for depositor and requestor alike... must be executed. The Golden Stopwatch Award highlights TTOs that accelerate science by processing agreements...

...Keima accumulates in autolysosomes, this ratio is high. When untargeted, Keima can be used to measure bulk...autophagy-related microtubule-associated proteins 1A/1B light chain 3B, or LC3. LC3 is a commonly used marker ...oligomerization Seeing red: simple GFP photoconversion Light sheet fluorescence microscopy Resources on Addgene.org...

... host-pathogen interface, previously developed a high-throughput neutralization assay to study the link...Pfizer or Modern vaccine. This sera was used in the high-throughput SARS-CoV-2 pseudovirus neutralization... KL, Nitido AD, Murali MR, Alter G, Charles RC, Dighe A, Branda JA, Lennerz JK, Lingwood D, Schmidt AG...

...forward. Here are a few out-of-the-box ideas that might jump start your learning. 1. Create a peer mentoring...what’s a scientist to do with no live audience in sight? You could do a talk virtually for your peer mentoring...university or use your current contacts to ask who they might suggest you speak with. See this post that’s all...

...their secondary structure, palindromic nature, and high GC content. This instability can cause partial loss...through ITRs due to their secondary structure and high GC content, but some companies now offer Sanger ...example, CAG is a strong promoter that drives very high, ubiquitous expression, while the human synapsin...

...Baradaran says.  Baradaran’s path to science began in high school. “I had a great chemistry teacher in 10th...medicines can have on human health. As a teenager in high school, I knew that this is what I wanted to do,...ClonTracer Barcoding Library, a library that “enables the high-resolution tracking of more than 1 million cancer...

...and it is under copyright, you will need to obtain written approval from the copyright owner and will ...treatment conditions like drugs (Chemogenetics) or light (Optogenetics) to activate receptors? If you’re ...

...the ‘bells and whistles’ – including microscale light emitting diodes, thermal sensors, and microfluidic...channels. These features enable the fibers to emit light for optogenetic purposes, measure temperature, study...construct expressing channelrhodopsin 2 (ChR2), a blue light sensitive cation channel, fused to mCherry to the...

...makes them want to know more. This could be by outright stating the big-picture problem, by asking your...science storytelling. You can introduce your results right at the beginning of your communication, use conflict...storytelling options, and they all can be effective in the right circumstances.   However, in the midst of all these...

...pBR322, pACYC, and pUC were developed to provide higher copy number vectors that could be used in these...updated and added new content to our eBook. Some highlights of the 2nd edition eBook include: new chapters...

...determine genome-wide translational frequency. Although highly useful, this technique requires extensive sample... nucleus to bind cognate untranslated mRNAs. The high specificity and resolution of this system are achieved...SunTag Catch up on Your GFP Background Choose the Brightest Fluorescent Protein Resources on Addgene.org ...

...ask is what to do with them. In some cases, there might be a mutation that disrupts the function of YFG....drivers. It is much easier to mate flies to get the right cell type expression as opposed to making a new ... my next post, I’ll explain the relatively straightforward process of generating your own fly lines. In...

...viral samples. Read on to learn how aqueous samples might be affected by dry ice, and specifically how dry... removing the caps, incubating the tubes in an upright position for 30 seconds, and then recapping the...to prevent any pH change, as we still observed a slight change in pH (from 7.4 to 6.8). It’s possible that...