We narrowed to 322 results for: BRE

1. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

   Type

   Blog Post

   Published

   May 26, 2020, 1:15 p.m.

   ...be targeted by a sgRNA guided Cas9 causing a DSB break. This DSB is repaired by NHEJ which depending on...

2. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

   Type

   Blog Post

   Published

   March 17, 2020, 1:32 p.m.

   ...the DNA base change without a DNA double-strand break. But base editors aren’t perfect. They may be slow...

3. Building and Validating Recombinant Antibodies: A Case Study

   Type

   Blog Post

   Published

   April 5, 2022, 1:20 p.m.

   ... Gong B, Nguyen K, van der List D, Murray KD, Engebrecht J, Trimmer JS. A toolbox of IgG subclass-switched...

4. Sweating the Small Stuff: Details in the Lab

   Type

   Blog Post

   Published

   Feb. 8, 2022, 2:15 p.m.

   ...intentionally slow down, you’re giving yourself a chance to break what can be a rather vicious cycle.  Everybody’s...

5. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

   Type

   Blog Post

   Published

   May 11, 2017, 2:30 p.m.

   ...Comprehensive Set of MAPK1/ERK2 Missense Mutants. Brenan L et al. Cell Reports, 2016. PubMed PMID: 27760319...

6. Top Requested Lentivirus and AAV of 2016

   Type

   Blog Post

   Published

   Jan. 6, 2017, 3:56 p.m.

   ... nuclease, which introduces double stranded DNA breaks, can be expressed from lentiCas9-Blast. Purpose...

7. Targeted Mutagenesis with EvolvR

   Type

   Blog Post

   Published

   Feb. 21, 2019, 1:15 p.m.

   ...tracing cells that do not tolerate double-strand DNA breaks by introducing a unique tag into a genomic site...

8. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

   Type

   Blog Post

   Published

   Feb. 19, 2019, 1:36 p.m.

   ... it available to everyone, that creates more breakthrough and innovation. With any kind of open platform...

9. Why and how to start studying chromatin (with Addgene's help)

   Type

   Blog Post

   Published

   June 12, 2018, 11:40 a.m.

   ...the histone methylation status at a double-strand break that needs repairing. They may be looking at chromatin...

10. Viral Vectors 101: Viral Vector Elements

    Type

    Blog Post

    Published

    July 25, 2023, 1 p.m.

    ...components are called helper plasmids. Below, we’ll breakdown the vector components first by virus and then ...

11. Viral Vectors 101: Types of viruses

    Type

    Blog Post

    Published

    June 6, 2023, 1:15 p.m.

    ...through nuclear pores and thus do not require breakdown of the nuclear envelope during cell division to...

12. Viral Vectors 101: The AAV Retrograde Serotype

    Type

    Blog Post

    Published

    April 27, 2023, 1:15 p.m.

    ...transduced at all by this serotype. Nichole Beebe in Brett Schofield’s lab demonstrated that pathways projecting...

13. Viral Vectors 101: Viral Applications

    Type

    Blog Post

    Published

    April 13, 2023, 1:15 p.m.

    ...Zaunders JJ, Gabuzda D, Crowe SM, Mills J, Lewin SR, Brew BJ, Cunningham AL, Churchill MJ. Pathogenicity and...

14. Viral Vectors 101: Viruses as Biological Tools

    Type

    Blog Post

    Published

    March 21, 2023, 1:15 p.m.

    ..., as their access to the host genome requires breakdown of the nuclear envelope, which occurs during mitosis...

15. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type

    Blog Post

    Published

    Aug. 6, 2024, 1:15 p.m.

    ...only able to enter the nucleus during mitotic breakdown of the nuclear envelope. Lentiviruses can transduce...

16. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

    Type

    Blog Post

    Published

    July 28, 2015, 2:30 p.m.

    ...offers high efficiency of inducing double strand breaks and more opportunity for homologous recombination...

17. Plasmids 101: Blue-white Screening

    Type

    Blog Post

    Published

    June 4, 2015, 1:03 p.m.

    ... When expressed, the β-galactosidase enzyme can break down a dye-linked substrate called x-gal (5-bromo...

18. How to Make Friends and Meet People at a Scientific Conference

    Type

    Blog Post

    Published

    Aug. 7, 2014, 1:58 p.m.

    ... you are interested in and this is a great ice breaker. Of course, real relationships go further and grow...

19. 3 Challenges in Plant Synthetic Biology

    Type

    Blog Post

    Published

    July 22, 2014, 5:46 p.m.

    ...the day cannot be developed through conventional breeding; synthetic biology is required to achieve this...

20. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type

    Blog Post

    Published

    July 10, 2014, 7:17 p.m.

    ...(A) Productize, (B) Roll out to market, and (C) Break even cycles. Work hard. Work fast. Be flexible. ...