We narrowed to 593 results for: ELP
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TypeBlog PostPublishedOct. 20, 2016, 2:30 p.m....pretty nicely displays her energetic, truthful and helpful style: Joanne also provided much more detailed...content for scientists in training is abundant, helpful, and sometimes excellent. Finally, as I said in... have questions? I am easy to reach and happy to help. Additional Resources on the Addgene Blog: 10 ...
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Plasmids 101: Control Plasmids
TypeBlog PostPublishedApril 9, 2015, 3:29 p.m....the functionality of your experimental system and help identify opportunities for troubleshooting or optimization...are control plasmids? Generally, control plasmids help to ensure that the observed phenomenon in your experiment...phenotype associated with the independent variable and helps ensure the phenotype isn’t singular or associated... -
Replacing Paper: Tips for Choosing an Electronic lab Notebook
TypeBlog PostPublishedJuly 10, 2018, 1:08 p.m....’s best efforts. We all have software tools that help us efficiently accomplish our daily goals. These...electronic lab notebooks into 9 questions that will help you start and define what exactly your lab needs... 21 CFR part 11, HIPAA? Answering this question helps you evaluate whether the ELN should have functionalities... -
Transferable Skills Guide: Time Management
TypeBlog PostPublishedDec. 5, 2017, 1:52 p.m....offering advice on areas where you might need some help. Today in our transferable skills guide: Time Mangement... made small accomplishments each day that should help keep you content and give you the energy to finish...day, writing your to-do list the night before will help keep you focused. For a real-world example, let... -
Antibodies 101: Introduction to Immunofluorescence
TypeBlog PostPublishedNov. 16, 2021, 2:15 p.m....step can involve multiple smaller steps that all help to ensure that antibodies can access their target...number of websites compiling antibody data that can help you compare antibodies from different companies,...Sharing your experience, positive or negative, will help make antibodies more effective and reliable in the... -
CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9
TypeBlog PostPublishedOct. 11, 2016, 2:30 p.m....plasmid, into which the GOI is inserted, and a Ti "helper" plasmid, which contains Vir genes necessary for...insertion into the plant genome. B) The Ti plasmid and helper are transformed into agrobacterium tumefaciens ...(yellow) are recognized by the Vir genes on the helper plasmid and used to insert the intervening region... -
Multiple Plasmids at a Low Price: Kits from Addgene
TypeBlog PostPublishedSept. 14, 2016, 2:30 p.m.... set of plasmids together on a tight budget. To help popularize our kit resources, we’re highlighting...selection markers built into all plasmids in the kit help decrease false positives observed in the cloning..." Small GTPases 7.2 (2016): 93-106. FREQ-Seq To help scientists track the frequencies of specific alleles... -
Antibodies 101: The Basics of Western Blotting
TypeBlog PostPublishedMarch 8, 2021, 2:41 p.m....of known molecular weight in a separate lane can help confirm the size of the protein of interest. An ... Visualizing your protein of interest with the help of secondary antibodies In order to visualize proteins...in your specific system. If you found this blog helpful be sure to check out Addgene’s blog for overviews... -
Antibodies 101: Multiplex Immunofluorescence
TypeBlog PostPublishedMay 23, 2023, 1:15 p.m.... to quantify accurately, but can be especially helpful to generate more signal from low abundance targets...individual panels, then the multicolor overlay; this helps all viewers, especially if you have some complex...improved variations you could try. We hope this post helped you start planning for your next multiplex IF experiment... -
A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression
TypeBlog PostPublishedNov. 2, 2021, 1:15 p.m....rate of the vector you are using. Contact Addgene (help@addgene.org), or the supplier of your vector, to...before beginning large scale experiments. This will help to ensure the accuracy and reproducibility of the...Monosynaptic Tracing Success Depends Critically on Helper Virus Concentrations. Front Synaptic Neurosci 12... -
Finding Your Science Policy Path
TypeBlog PostPublishedSept. 22, 2020, 1:15 p.m.... meet ups and happy hours that you can attend to help build relationships. Reach out to a science policy...important question that you can explore. The answer will help you decide how to define the who, what, where, when...challenges you’ve overcome. This information may help you determine what parts of your current job you... -
Custom CRISPR Screens & the Green Listed Software
TypeBlog PostPublishedJuly 11, 2017, 2:30 p.m....you need help putting your list together. Once you have your list of genes, Green Listed can help make the...CRISPR screen. Luckily, the Green Listed software can help you do just this, probably in less than a minute... -
Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs
TypeBlog PostPublishedDec. 20, 2017, 2:33 p.m....“put a face to a name” will help a candidate get looked at and should help facilitate the interview process...responsibilities during their careers - being aware of this can help take the pressure off having to find the perfect... -
New Antibody Animation!
TypeBlog PostPublishedFeb. 22, 2024, 3:13 p.m....about antibodies? Our latest animation is here to help! Join Abi as they explain what antibodies are, their...Subscribe to Addgene's YouTube channel for more helpful animations, useful protocol videos, and quick tips... -
New Antibodies in the Lab Animation!
TypeBlog PostPublishedNov. 19, 2024, 2:15 p.m....options are? Addgene's latest animation is here to help! "Antibodies 101: Antibodies in the Lab" shows ...watching, and we hope you found the video radd-ically helpful! More resources at Addgene Addgene's Antibody... -
Viral Vectors 101 eBook 2nd Edition
TypeBlog PostPublishedJuly 11, 2024, 1:15 p.m....practical guide to using viral vectors in the lab is a helpful resource for bench scientists everywhere. In it...different types of vectors, capsids, and tools; and helpful information on everything from producing your own... -
Suggest A Plasmid You'd Like to Find at Addgene
TypeBlog PostPublishedMarch 16, 2016, 5:30 p.m....find the plasmid at Addgene? Well, we’re here to help with our Suggest a Plasmid Campaign. How to suggest...suggest and include any additional details that will help us reach out to the corresponding potential depositors... -
New FLIS Plasmid (April Fools 2022)
TypeBlog PostPublishedApril 1, 2022, 1:15 p.m....at regular intervals. This innovation system can help fly labs everywhere find and re-contain any specimens...research, I hope you find this new technology as helpful as we have! References and Resources Additional... -
Participate in the Pets of Addgene Scavenger Hunt
TypeBlog PostPublishedApril 1, 2021, 1:15 p.m....blog. Below each photo, the pet gives you a clue to help you find the next picture. If you find the next ...newly updated navigation menu and search tool to help you navigate the scavenger hunt. Good luck and have... -
CRISPR 101 eBook...en español
TypeBlog PostPublishedJan. 18, 2024, 2:15 p.m....including working with distributors across the world to help increase global access to materials in our repository...incredible gift will allow more people to access helpful resources for their CRISPR experiments. Many thanks...