...all available sequence information from Addgene depositors and our own sequencing results: On this page ...sequence in your sequence viewer of choice. If the depositor provided a custom annotated GenBank file, this...updates will make it much easier to browse the repository and quickly identify the plasmids and features...

...Biotechnologies' press release about their Cas13d deposit here). Konermann et al.: CasRx is a highly potent...localization signal (NLS). In a HEK293 mCherry reporter assay, CasRx and AdmCas13d produced 92% and 87%...continue to grow, and we at Addgene appreciate our depositors for making their tools available to the broader...

...promoter, thereby activating transcription. Addgene depositors have developed heat shock-inducible Cre and Cas9... phage promoter pR, preventing expression of a reporter gene. When light is present, YFI is inactive, ...preventing repressor cI synthesis and allowing reporter gene transcription to take place. Addgene also...

...entire mission is to enable that process. Our repository of plasmids, AAVs, antibodies, and data allows...and cited figures from papers or image sharing repositories. Then we format, do a final review to catch ...informative resources that answer questions our depositors and customers are asking. So how can you get ...

... an ERK kinase translocation reporter with a mRuby2 fluorescent reporter (ERK KTR-mRuby2). The ERK KTR...Activity with GFP Resources at Addgene.org Find KTR reporter plasmids Find photoactivatable fluorescent proteins...

...pH-sensitive fluorescent biosensor that was recently deposited with Addgene by Dr. Mark Prescott. This system...2008). Rosella: A fluorescent pH-biosensor for reporting vacuolar turnover of cytosol and organelles in...using a single fluorescent biosensor. Scientific Reports,5(1). doi:10.1038/srep12397. PubMed PMID: 26215030...

...do? Let’s take a look at how plasmids recently deposited by Chris Voigt’s lab help keep gene expression...buffered against the effects of copy number on reporter gene expression. On the other hand, using a constitutive...Download the Plasmid 101 eBook See all plasmids deposited by the Voigt lab ...

...by reducing the distance between the target and reporter. They have fewer non-specific binding opportunities...it either needs to be directly conjugated to a reporter or you need a light-chain specific conjugated ...fairly easy to find these in a Fab fragment format. Reporter-conjugated anti-IgG Fab fragments make good secondaries...

...community. This is no small task, however, at a repository that consistently receives around 200 new DNA...data on every plasmid we distribute through our deposit process, and then we carefully track samples through...your effort. In addition, if you should choose to deposit your plasmids with us, it will be easy to find ...

...researcher, Carrie Bhang. The ClonTracer Library, deposited by Carrie Bhang, a research investigator in the...to EGFR inhibitor (Hata et al., 2016).  Bhang deposited the library with Addgene because she wanted to...sharing protocols one by one,” and she is excited to deposit the library with Addgene because it is a “really...

...vector blog posts for updates on the most recent deposits, protocols, and tips for using retroviral vectors...Plasmid #17408)   This is a lentiviral vector deposited by Dr. Tyler Jacks. It carries CRE recombinase...together with expression of fluorescent or enzymatic reporters for cellular assays and animal imaging studies...

...PMM2 gene. Videira’s group recently reviewed and reported that PMM2-CDG patients suffer from immunological...drugs can be used to treat these diseases (drug repositioning). The ultimate goal of this work is to lower... construct NFIX expression vectors as well as reporters of NFIX function. They will then mutagenize the...

...With our ever growing rate of plasmid deposits (over 100 deposits per month!), manual transformations were...Management Software for Biology Tips from the Repository Trenches: Using Barcodes to Track Samples Resources...

...optogenetic reporter, channelrhodopsin, fused to a fluorescent GFP marker (CatCH-GFP). The lab deposited AAV ...expression of a Cre-dependent fluorescent CatCH-GFP reporter. Expression of GFP will only result in cells where...

...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to ...recently contributed a set of plasmids to the repository that will help epigenetic researchers custom ...

...scientists who were potential Addgene requestors and depositors. China is in one of the top 10 countries in number...was kicked off by Nobel Laureate (and Addgene depositor) Craig Mello. Taking his groundbreaking work in... labs are using CRISPR/cas9 and more are being reported. Zhou Songyang from Baylor College of Medicine...

...As the Chair of the Advocacy Committee, I had to report to the Board of Directors and liaise with the Executive...accordingly. For example, the quarterly and annual reports I had to write on behalf of the Advocacy Committee...to overcome the personal frustration and submit reports in the format requested which in the long run was...

...magnetic fields (Frank Hayward et al., 2024). They deposited their plasmid with Addgene, hoping to inspire .... Andrew and Maria were encouraged by previous reports that described magnetic responses of cryptochrome...reproduced their EGFP findings since the initial report in 2023.)     Figure 1: Fluorescence intensity...

...Scientific Reports paper introducing the delivery method in 2014, Manuel Goncalves lab report that AdV-... protocol. The three plasmids which have been deposited to Addgene are: pAdSh.PGK.Cas9, pAdSh.U6.gRNAS1...

...the sequencing results of the plasmids in our repository with known reference sequences, such as full ...plasmid sequences provided by the laboratories that deposit their plasmids with us or other entries in NCBI...NCBI’s numerous databases. As our repository has grown over the years (we now have over 60,000 plasmids!...