We narrowed to 303 results for: GAI

1. Open Resources and Plasmid Tools For Studying C. elegans

   Type

   Blog Post

   Published

   July 18, 2019, 12:55 p.m.

   ...the 22nd International C. elegans meeting, I was again reminded of the extent that C. elegans researchers...

2. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

   Type

   Blog Post

   Published

   May 7, 2020, 1:15 p.m.

   ...multiple genes to generate a cellular phenotype. Again ABE8.20-m was the winner. It could simultaneously...

3. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

   Type

   Blog Post

   Published

   Dec. 14, 2021, 2:15 p.m.

   ...existed. Recently, however, a particular tool has gained widespread popularity – and notoriety – as a tool...

4. Viral Vectors 101: Chemogenetics

   Type

   Blog Post

   Published

   July 11, 2023, 1:15 p.m.

   ...deschloroclozapine (DCZ) shows promise as a CNO alternative (Nagai et al., 2019). Regardless of which ligand you choose...

5. Antibodies 101: Stripping and Reprobing Western Blots

   Type

   Blog Post

   Published

   Aug. 1, 2024, 1:15 p.m.

   ...from your membrane, allowing you to probe the blot again, almost like new. This approach preserves your sample...

6. Hot Plasmids February 2024

   Type

   Blog Post

   Published

   Feb. 20, 2024, 2:45 p.m.

   ... is not neutralized by pre-existing antibodies against AAV9-based vectors (and vice versa), enabling repeat...

7. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

   Type

   Blog Post

   Published

   Sept. 13, 2016, 2:30 p.m.

   ...departments across the university. Collective bargaining may provide a better deal for students in areas...

8. Cpf1 Update: Comparison to Cas9 and NgAgo

   Type

   Blog Post

   Published

   July 14, 2016, 2:30 p.m.

   ... of the PAM may give Cpf1 the ability to cleave again and possibly mediate HDR in a “second chance” mechanism...

9. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

   Type

   Blog Post

   Published

   Dec. 1, 2015, 3:30 p.m.

   ...desribed how P. furiosus has systems to defend against both DNA and RNA viruses - 2 cas systems regulate...

10. Illuminating Epigenetics with A FRET Based Biosensor

    Type

    Blog Post

    Published

    Nov. 19, 2015, 3:30 p.m.

    ...fluorescent units and so lowers the FRET signal back again. The Ting lab constructed 2 versions of this reporter...

11. The Materials Science of Optogenetics Experiments

    Type

    Blog Post

    Published

    Sept. 17, 2015, 2:30 p.m.

    ...opportunity to “reset” and become receptive to light once again. Therefore, light must be delivered in pulses. An...

12. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type

    Blog Post

    Published

    March 28, 2018, 6:52 p.m.

    ...were capable of cleavage, they tested the clones against an NNN PAM library in bacteria where cleavage leads...

13. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type

    Blog Post

    Published

    Sept. 12, 2017, 1:44 p.m.

    ...to the contaminant. Each contig is then BLASTed against the NCBI nucleotide collection database, and it...

14. Cloning Mammalian Cells with the Agarose Method

    Type

    Blog Post

    Published

    Sept. 7, 2017, 12:17 p.m.

    ...We test the (capped) 1:1 agarose/DMEM solution against a part of the body very sensitive to heat- the ...

15. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

    Type

    Blog Post

    Published

    June 22, 2017, 2:30 p.m.

    ...However, much knowledge about functionality can be gained by understanding the location and transport of ...

16. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type

    Blog Post

    Published

    April 4, 2019, 12:53 p.m.

    ...recipient's genome. Antibiotic plates lacking DAP select against donor bacteria and for recipients with an integrated...

17. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type

    Blog Post

    Published

    Feb. 7, 2019, 2:16 p.m.

    ...University in Canada. He's happy to be contributing again to science.   Acknowledgements Chan Zuckerberg ...

18. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type

    Blog Post

    Published

    July 2, 2020, 12:40 p.m.

    ...Next Experiment Learn More About Designing gRNAs Against Your Favorite CREs Here Additional Resources on...

19. PCR Applications for Copy Number Variation Assays

    Type

    Blog Post

    Published

    April 26, 2022, 1:15 p.m.

    ...price point, which should be carefully balanced against the limitations of the assay.      Fig....

20. Transferable Skills: Negotiation

    Type

    Blog Post

    Published

    March 3, 2022, 2 p.m.

    ... company. How can I negotiate with my manager to gain experience in the area I want to explore while also...