We narrowed to 307 results for: GAI

1. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

   Type

   Blog Post

   Published

   May 22, 2018, 1:38 p.m.

   ... who participate in the course show significant gains in both content knowledge and research skills (8...

2. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

   Type

   Blog Post

   Published

   May 1, 2018, 1:57 p.m.

   ...with an antibody. This can artificially select against PPIs that aren't stable outside the native cell...

3. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

   Type

   Blog Post

   Published

   Sept. 26, 2019, 12:50 p.m.

   ...transduction; but these methods aren’t stealthy enough to gain access to some cells and tissues, such as human ...

4. Open Resources and Plasmid Tools For Studying C. elegans

   Type

   Blog Post

   Published

   July 18, 2019, 12:55 p.m.

   ...the 22nd International C. elegans meeting, I was again reminded of the extent that C. elegans researchers...

5. Hot Plasmids February 2024

   Type

   Blog Post

   Published

   Feb. 20, 2024, 2:45 p.m.

   ... is not neutralized by pre-existing antibodies against AAV9-based vectors (and vice versa), enabling repeat...

6. Viral Vectors 101: Chemogenetics

   Type

   Blog Post

   Published

   July 11, 2023, 1:15 p.m.

   ...deschloroclozapine (DCZ) shows promise as a CNO alternative (Nagai et al., 2019). Regardless of which ligand you choose...

7. Antibodies 101: Stripping and Reprobing Western Blots

   Type

   Blog Post

   Published

   Aug. 1, 2024, 1:15 p.m.

   ...from your membrane, allowing you to probe the blot again, almost like new. This approach preserves your sample...

8. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

   Type

   Blog Post

   Published

   Sept. 13, 2016, 2:30 p.m.

   ...departments across the university. Collective bargaining may provide a better deal for students in areas...

9. Cpf1 Update: Comparison to Cas9 and NgAgo

   Type

   Blog Post

   Published

   July 14, 2016, 2:30 p.m.

   ... of the PAM may give Cpf1 the ability to cleave again and possibly mediate HDR in a “second chance” mechanism...

10. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type

    Blog Post

    Published

    Dec. 1, 2015, 3:30 p.m.

    ...desribed how P. furiosus has systems to defend against both DNA and RNA viruses - 2 cas systems regulate...

11. Illuminating Epigenetics with A FRET Based Biosensor

    Type

    Blog Post

    Published

    Nov. 19, 2015, 3:30 p.m.

    ...fluorescent units and so lowers the FRET signal back again. The Ting lab constructed 2 versions of this reporter...

12. The Materials Science of Optogenetics Experiments

    Type

    Blog Post

    Published

    Sept. 17, 2015, 2:30 p.m.

    ...opportunity to “reset” and become receptive to light once again. Therefore, light must be delivered in pulses. An...

13. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type

    Blog Post

    Published

    July 2, 2020, 12:40 p.m.

    ...Next Experiment Learn More About Designing gRNAs Against Your Favorite CREs Here Additional Resources on...

14. PCR Applications for Copy Number Variation Assays

    Type

    Blog Post

    Published

    April 26, 2022, 1:15 p.m.

    ...price point, which should be carefully balanced against the limitations of the assay.      Fig....

15. Transferable Skills: Negotiation

    Type

    Blog Post

    Published

    March 3, 2022, 2 p.m.

    ... company. How can I negotiate with my manager to gain experience in the area I want to explore while also...

16. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type

    Blog Post

    Published

    March 28, 2018, 6:52 p.m.

    ...were capable of cleavage, they tested the clones against an NNN PAM library in bacteria where cleavage leads...

17. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type

    Blog Post

    Published

    April 4, 2019, 12:53 p.m.

    ...recipient's genome. Antibiotic plates lacking DAP select against donor bacteria and for recipients with an integrated...

18. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type

    Blog Post

    Published

    Feb. 7, 2019, 2:16 p.m.

    ...University in Canada. He's happy to be contributing again to science.   Acknowledgements Chan Zuckerberg ...

19. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

    Type

    Blog Post

    Published

    June 22, 2017, 2:30 p.m.

    ...However, much knowledge about functionality can be gained by understanding the location and transport of ...

20. Hot Plasmids: Summer 2025

    Type

    Blog Post

    Published

    Aug. 28, 2025, 1:15 p.m.

    ...TnpB has found itself in the spotlight. TnpBs are gaining popularity, especially among plant biologists, ...