We narrowed to 253 results for: IRES

1. Plasmids 101: Shuttle Vectors

   Type

   Blog Post

   Published

   May 20, 2025, 1:15 p.m.

   ...helps increase cellular uptake, but this approach requires compatible cell lines that express viral machinery...

2. Plasmids 101: Broad Host Range Plasmids

   Type

   Blog Post

   Published

   April 11, 2024, 1:15 p.m.

   ...pneumophila, Methylophilus methylotrophus, Myxococcus virescens, M. xanthus, Proteus rettgeri, P. mirabilis, Providencia...

3. How to Write a Scientific Review Article

   Type

   Blog Post

   Published

   Feb. 16, 2017, 3:30 p.m.

   ...convert the citation style to whatever the journal requires. Using the (Last name, Year) format also has the...

4. Plasmids 101: Gateway Cloning

   Type

   Blog Post

   Published

   Jan. 12, 2017, 3:30 p.m.

   ...and destination vector creates two products: the desires expression clone and a byproduct containing the...

5. Antibiotic Resistance: An Old Solution but a New Problem

   Type

   Blog Post

   Published

   Nov. 10, 2016, 3:30 p.m.

   ...Kirby-Bauer susceptibility test (Figure 2), and requires at least 18-24 hours to obtain results. While ...

6. Overcoming the AAV Size Limitation for CRISPR Delivery

   Type

   Blog Post

   Published

   Sept. 16, 2020, 1 p.m.

   ...drawbacks limit the utility of these systems: St1Cas9 requires a very specific PAM sequence that limits the number...

7. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

   Type

   Blog Post

   Published

   July 7, 2020, 1:15 p.m.

   ...different reporter proteins. Protein translation requires the start codon of AUG immediately downstream ...

8. Analyzing CRISPR Editing Results with ICE from Synthego

   Type

   Blog Post

   Published

   May 8, 2018, 1 p.m.

   ...generating reliable results from CRISPR edit data requires the help of robust software tools. As a consequence...

9. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

   Type

   Blog Post

   Published

   Dec. 14, 2017, 2:08 p.m.

   ...passages. Furthermore, working with Sendai virus requires stringent biosafety measures, thereby increasing...

10. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

    Type

    Blog Post

    Published

    July 23, 2019, 12:46 p.m.

    ... following injection into the bloodstream, but requires a high vector dose and is unable to reach all ...

11. New Tools Enable CRISPRa for Neuroscience Applications

    Type

    Blog Post

    Published

    July 2, 2019, 12:51 p.m.

    ...., 2018). The pooled nature of this experiment requires fluorescence activated cell sorting (FACS) to ...

12. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type

    Blog Post

    Published

    May 2, 2019, 12:41 p.m.

    ...is no small task. The EDGE program solicitation requires funded labs to rapidly disseminate protocols, ...

13. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type

    Blog Post

    Published

    Nov. 19, 2020, 2:15 p.m.

    ...Samaco RC, Zoghbi HY (2011) Adult Neural Function Requires MeCP2. Science 333:186–186 . https://doi.org/10.1126...

14. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type

    Blog Post

    Published

    Oct. 13, 2022, 1:45 p.m.

    ...initiation. In mammalian cells, robust repression requires targeting dCas9 along with transcriptional repressors...

15. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type

    Blog Post

    Published

    April 8, 2025, 1:15 p.m.

    ...housekeeping proteins.” Fully quantifying a western blot requires a standard curve with a pure sample of your protein...

16. Antibodies 101: Beyond Surface Labeling

    Type

    Blog Post

    Published

    June 27, 2024, 1:15 p.m.

    ... hence, intracellular labeling with antibodies requires chemically pre-treating the cells to allow for...

17. Tips for Getting a Faculty Position

    Type

    Blog Post

    Published

    July 20, 2023, 1:15 p.m.

    ...really are selling yourself. A sales pitch that requires reading two pages or even a paragraph to get to...

18. Hot Biosensors 2022: Year-End Roundup

    Type

    Blog Post

    Published

    Dec. 6, 2022, 2:15 p.m.

    ...variations and heat transfer at the subcellular level requires a probe with fast kinetics and high signal/noise...

19. March for Science

    Type

    Blog Post

    Published

    April 21, 2017, 2:30 p.m.

    ...science rallies scientists to political action, and inspires politicians and citizens to include science in...

20. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type

    Blog Post

    Published

    Feb. 22, 2017, 3:30 p.m.

    ...phage-derived uracil labeled template, this method requires further DNA shuffling to combine pairs of mutations...