Addgene

We narrowed to 249 results for: IRES

Showing: 201 - 220 of 249 results

Results per page:

Analyzing CRISPR Editing Results with ICE from Synthego

Type

Blog Post

Published

May 8, 2018, 1 p.m.

...generating reliable results from CRISPR edit data requires the help of robust software tools. As a consequence...
pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

Type

Blog Post

Published

Dec. 14, 2017, 2:08 p.m.

...passages. Furthermore, working with Sendai virus requires stringent biosafety measures, thereby increasing...
Plasmids 101: Shuttle Vectors

Type

Blog Post

Published

May 20, 2025, 1:15 p.m.

...helps increase cellular uptake, but this approach requires compatible cell lines that express viral machinery...
How to Write a Scientific Review Article

Type

Blog Post

Published

Feb. 16, 2017, 3:30 p.m.

...convert the citation style to whatever the journal requires. Using the (Last name, Year) format also has the...
Plasmids 101: Gateway Cloning

Type

Blog Post

Published

Jan. 12, 2017, 3:30 p.m.

...and destination vector creates two products: the desires expression clone and a byproduct containing the...
Antibiotic Resistance: An Old Solution but a New Problem

Type

Blog Post

Published

Nov. 10, 2016, 3:30 p.m.

...Kirby-Bauer susceptibility test (Figure 2), and requires at least 18-24 hours to obtain results. While ...
Plasmids 101: Broad Host Range Plasmids

Type

Blog Post

Published

April 11, 2024, 1:15 p.m.

...pneumophila, Methylophilus methylotrophus, Myxococcus virescens, M. xanthus, Proteus rettgeri, P. mirabilis, Providencia...
CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

Type

Blog Post

Published

Oct. 13, 2022, 1:45 p.m.

...initiation. In mammalian cells, robust repression requires targeting dCas9 along with transcriptional repressors...
Rett Syndrome: A History of Research and Therapeutic Outlooks

Type

Blog Post

Published

Nov. 19, 2020, 2:15 p.m.

...Samaco RC, Zoghbi HY (2011) Adult Neural Function Requires MeCP2. Science 333:186–186 . https://doi.org/10.1126...
Overcoming the AAV Size Limitation for CRISPR Delivery

Type

Blog Post

Published

Sept. 16, 2020, 1 p.m.

...drawbacks limit the utility of these systems: St1Cas9 requires a very specific PAM sequence that limits the number...
Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

Type

Blog Post

Published

July 7, 2020, 1:15 p.m.

...different reporter proteins. Protein translation requires the start codon of AUG immediately downstream ...
Antibodies 101: Introduction to Immunofluorescence

Type

Blog Post

Published

Nov. 16, 2021, 2:15 p.m.

...visualize their target. In contrast, indirect IF requires two antibodies: a primary and secondary. For indirect...
Antibodies 101: Single Chain Fragment Variables (scFvs)

Type

Blog Post

Published

June 3, 2021, 1:15 p.m.

...for the scFv (Gaciarz and Ruddock., 2017), but requires an oxidizing environment to form. When expressed...
The Fluorescent Vegetables in Aptamer Soup

Type

Blog Post

Published

Jan. 29, 2019, 2:27 p.m.

...instability at 37°C (Ouellet et al., 2016) and requires a tRNA scaffold to improve its stability in vivo...
Tips for Getting a Faculty Position

Type

Blog Post

Published

July 20, 2023, 1:15 p.m.

...really are selling yourself. A sales pitch that requires reading two pages or even a paragraph to get to...
Antibodies 101: Normalization and Loading Controls for Western Blots

Type

Blog Post

Published

April 8, 2025, 1:15 p.m.

...housekeeping proteins.” Fully quantifying a western blot requires a standard curve with a pure sample of your protein...
Antibodies 101: Beyond Surface Labeling

Type

Blog Post

Published

June 27, 2024, 1:15 p.m.

... hence, intracellular labeling with antibodies requires chemically pre-treating the cells to allow for...
March for Science

Type

Blog Post

Published

April 21, 2017, 2:30 p.m.

...science rallies scientists to political action, and inspires politicians and citizens to include science in...
Deep Mutational Scanning with One Pot Saturation Mutagenesis

Type

Blog Post

Published

Feb. 22, 2017, 3:30 p.m.

...phage-derived uracil labeled template, this method requires further DNA shuffling to combine pairs of mutations...
Site Directed Mutagenesis by PCR

Type

Blog Post

Published

Aug. 2, 2016, 2:30 p.m.

...double-stranded break. This site-directed genome editing requires a template of high homology to the endogenous ...

Showing: 201 - 220 of 249 results

Results per page:

Analyzing CRISPR Editing Results with ICE from Synthego

pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

Plasmids 101: Shuttle Vectors

How to Write a Scientific Review Article

Plasmids 101: Gateway Cloning

Antibiotic Resistance: An Old Solution but a New Problem

Plasmids 101: Broad Host Range Plasmids

CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

Rett Syndrome: A History of Research and Therapeutic Outlooks

Overcoming the AAV Size Limitation for CRISPR Delivery

Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

Antibodies 101: Introduction to Immunofluorescence

Antibodies 101: Single Chain Fragment Variables (scFvs)

The Fluorescent Vegetables in Aptamer Soup

Tips for Getting a Faculty Position

Antibodies 101: Normalization and Loading Controls for Western Blots

Antibodies 101: Beyond Surface Labeling

March for Science

Deep Mutational Scanning with One Pot Saturation Mutagenesis

Site Directed Mutagenesis by PCR

Help Center

Deposit Plasmids

General Reagents

Viral Service

DNA Service

Plasmid Collections