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We narrowed to 249 results for: IRES

Showing: 201 - 220 of 249 results

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CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

Type

Blog Post

Published

Oct. 13, 2022, 1:45 p.m.

...initiation. In mammalian cells, robust repression requires targeting dCas9 along with transcriptional repressors...
What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

Type

Blog Post

Published

May 2, 2019, 12:41 p.m.

...is no small task. The EDGE program solicitation requires funded labs to rapidly disseminate protocols, ...
Rett Syndrome: A History of Research and Therapeutic Outlooks

Type

Blog Post

Published

Nov. 19, 2020, 2:15 p.m.

...Samaco RC, Zoghbi HY (2011) Adult Neural Function Requires MeCP2. Science 333:186–186 . https://doi.org/10.1126...
Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

Type

Blog Post

Published

July 7, 2020, 1:15 p.m.

...different reporter proteins. Protein translation requires the start codon of AUG immediately downstream ...
Overcoming the AAV Size Limitation for CRISPR Delivery

Type

Blog Post

Published

Sept. 16, 2020, 1 p.m.

...drawbacks limit the utility of these systems: St1Cas9 requires a very specific PAM sequence that limits the number...
Plasmids 101: Shuttle Vectors

Type

Blog Post

Published

May 20, 2025, 1:15 p.m.

...helps increase cellular uptake, but this approach requires compatible cell lines that express viral machinery...
Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

Type

Blog Post

Published

July 23, 2019, 12:46 p.m.

... following injection into the bloodstream, but requires a high vector dose and is unable to reach all ...
New Tools Enable CRISPRa for Neuroscience Applications

Type

Blog Post

Published

July 2, 2019, 12:51 p.m.

...., 2018). The pooled nature of this experiment requires fluorescence activated cell sorting (FACS) to ...
How to Write a Scientific Review Article

Type

Blog Post

Published

Feb. 16, 2017, 3:30 p.m.

...convert the citation style to whatever the journal requires. Using the (Last name, Year) format also has the...
Plasmids 101: Gateway Cloning

Type

Blog Post

Published

Jan. 12, 2017, 3:30 p.m.

...and destination vector creates two products: the desires expression clone and a byproduct containing the...
Antibiotic Resistance: An Old Solution but a New Problem

Type

Blog Post

Published

Nov. 10, 2016, 3:30 p.m.

...Kirby-Bauer susceptibility test (Figure 2), and requires at least 18-24 hours to obtain results. While ...
Antibodies 101: Beyond Surface Labeling

Type

Blog Post

Published

June 27, 2024, 1:15 p.m.

... hence, intracellular labeling with antibodies requires chemically pre-treating the cells to allow for...
Tips for Getting a Faculty Position

Type

Blog Post

Published

July 20, 2023, 1:15 p.m.

...really are selling yourself. A sales pitch that requires reading two pages or even a paragraph to get to...
Hot Biosensors 2022: Year-End Roundup

Type

Blog Post

Published

Dec. 6, 2022, 2:15 p.m.

...variations and heat transfer at the subcellular level requires a probe with fast kinetics and high signal/noise...
The Fluorescent Vegetables in Aptamer Soup

Type

Blog Post

Published

Jan. 29, 2019, 2:27 p.m.

...instability at 37°C (Ouellet et al., 2016) and requires a tRNA scaffold to improve its stability in vivo...
Antibodies 101: Introduction to Immunofluorescence

Type

Blog Post

Published

Nov. 16, 2021, 2:15 p.m.

...visualize their target. In contrast, indirect IF requires two antibodies: a primary and secondary. For indirect...
Antibodies 101: Single Chain Fragment Variables (scFvs)

Type

Blog Post

Published

June 3, 2021, 1:15 p.m.

...for the scFv (Gaciarz and Ruddock., 2017), but requires an oxidizing environment to form. When expressed...
SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

Type

Blog Post

Published

May 5, 2020, 1:15 p.m.

...sequence preferences at the target site, while Cas12a requires a PAM (Protospacer Adjacent Motif) for target ...
Replacing Paper: Tips for Choosing an Electronic lab Notebook

Type

Blog Post

Published

July 10, 2018, 1:08 p.m.

...switching from paper to an electronic lab notebook requires time and knowledge. In a recent article, we translated...
Antibodies 101: Normalization and Loading Controls for Western Blots

Type

Blog Post

Published

April 8, 2025, 1:15 p.m.

...housekeeping proteins.” Fully quantifying a western blot requires a standard curve with a pure sample of your protein...

Showing: 201 - 220 of 249 results

Results per page:

CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

Rett Syndrome: A History of Research and Therapeutic Outlooks

Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

Overcoming the AAV Size Limitation for CRISPR Delivery

Plasmids 101: Shuttle Vectors

Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

New Tools Enable CRISPRa for Neuroscience Applications

How to Write a Scientific Review Article

Plasmids 101: Gateway Cloning

Antibiotic Resistance: An Old Solution but a New Problem

Antibodies 101: Beyond Surface Labeling

Tips for Getting a Faculty Position

Hot Biosensors 2022: Year-End Roundup

The Fluorescent Vegetables in Aptamer Soup

Antibodies 101: Introduction to Immunofluorescence

Antibodies 101: Single Chain Fragment Variables (scFvs)

SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

Replacing Paper: Tips for Choosing an Electronic lab Notebook

Antibodies 101: Normalization and Loading Controls for Western Blots

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