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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 461 results for: MEA;

Showing: 201 - 220 of 461 results

  1. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ...should be available for anonymous request.  This means that supporting datasets must be logically annotated...will be in danger of having to deliver on well-meaning but impractical requirements imposed by publishers...

  2. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    Published
    April 3, 2014, 8:05 p.m.
    ...recognize and bind to specific promoter elements. This means that the promoter present in your plasmid backbone...bacterial and mammalian promoters. These lists is by no means exhaustive, but should be a good place to start ...

  3. Transferable Skills Guide: Time Management

    Type
    Blog Post
    Published
    Dec. 5, 2017, 1:52 p.m.
    ... be aware of deadlines and think about what they mean in terms of the utility of your tasks. In my cell...accomplish these goals. Practically speaking, this means I write everything in a Word or google doc that ...

  4. Tips for Getting a Faculty Position

    Type
    Blog Post
    Published
    July 20, 2023, 1:15 p.m.
    ...rapidly dispelled. She would ask me, "Is this what you mean? Are you trying to say this?" I'd say, "No, I'm ...a lab, getting grants, and writing papers? This means that once you get an interview, your previous success...

  5. Evolution of Lab Techniques

    Type
    Blog Post
    Published
    June 21, 2016, 2:30 p.m.
    ...DNA strand as it slips through a tiny pore by measuring differences in ion flow caused by the different...nanopore-based DNA sequencing machines are relatively small meaning that DNA sequencing could move from sequencing ...

  6. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ...proteins can be accurately determined in vitro by measuring the fluorescence intensity of purified protein..., Marieke, et al. "A FRET-based biosensor for measuring Gα13 activation in single cells." PloS one 13.3...

  7. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    Published
    Nov. 16, 2021, 2:15 p.m.
    ...secondary antibodies are generally polyclonal, which means that multiple secondary molecules can bind to a ...target and sample type. Example steps include: permeabilization, antigen retrieval, and sectioning. Blocking...

  9. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ...β-galactosidase activity abolished   lacY Lactose permease inactivated, lactose cannot be taken up by cell...common lab strains; however, these tables are by no means exhaustive! For a more comprehensive list and additional...

  12. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ...then prevents transcription from occurring, which means that transcription is now OFF.   We’ll look at ... 3 binary systems listed above are orthogonal, meaning that they do not affect each other. For example...

  13. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    Published
    March 8, 2021, 2:41 p.m.
    ... western. To prepare the samples for SDS-PAGE, measure the protein content and normalize to ensure equivalent...gauge protein abundance they provide qualitative measurements. If you require precise quantification of a ...

  14. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ...target within ~30 nt of your proposed edit, which means there are so few gRNAs to choose from that sequence...of the repair template (Yang et al., 2013). This means that, for gene editing, there are usually few potential...

  15. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    Published
    Aug. 5, 2025, 1:15 p.m.
    ... the clinic. At the bench, improving antibodies means improving research. More specific antibodies support...display are common. ELISA enables quantitative measurement of antigen-antibody interactions in a plate-based...

  17. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ...mechanisms of action and other analyses. This also means that between two types, the Cas effector may have...nature24049 Altae-Tran, H., Kannan, S., Suberski, A. J., Mears, K. S., Demircioglu, F. E., Moeller, L., Kocalar...

  18. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Published
    April 25, 2017, 2:30 p.m.
    ...and inactivates Dronpa is not ideal, since this means Dronpa is quickly inactivated and less photons are...fluorescence the better. More fluorescence usually means more photons are being emitted, making it easier...

  19. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    Published
    Oct. 10, 2017, 1:57 p.m.
    ...microscope before it reaches the detector. This means that two or more objects lying within the diffraction...axial dimension that will appear in focus). This means that the images will appear sharp without interference...

  20. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    Published
    May 27, 2025, 1:15 p.m.
    ... etc. (more on prep sizes below). But what do we mean by DNA quality? There are a few different issues...?  Obtaining higher quantities of DNA generally means starting with higher volumes of bacterial culture...
Showing: 201 - 220 of 461 results