We narrowed to 459 results for: SHI
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TypeBlog PostPublishedNov. 10, 2022, 2:15 p.m.... biological system of interest, the so-called “fishing expedition.” Your first question is: to screen ...library resources or our RNAi resources and go fishing! References and resources References Wang, T.,...
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Antibodies 101: Monoclonal Antibodies
TypeBlog PostPublishedJune 29, 2021, 1:15 p.m....2005) Monoclonal Versus Polyclonal Antibodies: Distinguishing Characteristics, Applications, and Information...Monoclonal Antibodies. Monoclonal Antibody Production. Washington (DC): National Academies Press (US); 1999. 1,... -
Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems
TypeBlog PostPublishedMay 19, 2020, 1:15 p.m....in the production of pseudotyped VSV particles (Fukushi et al., 2008). Browse the Ginkgo Bioworks COVID...COVID-19 Plasmids and Resources Page! References Fukushi S, Watanabe R, Taguchi F (2008) Pseudotyped Vesicular... -
Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur
TypeBlog PostPublishedJuly 10, 2014, 7:17 p.m.... Sample6 has changed focus and names, swapped leadership, and grown to 25 members. What is Sample6?...money to sustain a startup, he went to work for Flagship Ventures – a local Cambridge biotech venture capital... -
Addgene: The First Twenty Years
TypeBlog PostPublishedDec. 19, 2024, 2:15 p.m....focus of our first decade. We did this the old-fashioned way: through physical outreach, visiting organizations... our repository grew to over 150,000 plasmids, shipped out our two millionth item, and had a cumulative... -
Transferable Skills Guide: Problem Solving
TypeBlog PostPublishedNov. 30, 2021, 2:15 p.m....systematically and logically is as useful for finishing your thesis as it is for helping your team reach... organization Time management Managing a team Leadership Resources on Addgene.org Check out our careers... -
Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number
TypeBlog PostPublishedSept. 4, 2018, 12:53 p.m....cause changes in plasmid copy number. During the shift from exponential to stationary phase, the number... able to better modulate gene expression during shifts in growth rate as compared to a constitutive promoter... -
The Breast Cancer Microenvironment: A Tumor’s Backstage Team
TypeBlog PostPublishedJan. 31, 2019, 1:32 p.m....long distances. When it comes to breast cancer, Hoshinho and colleagues found that after injecting breast...cell 21.3 (2012): 309-322. PubMed PMID: 22439926. Hoshino, Ayuko, et al. "Tumour exosome integrins determine... -
Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics
TypeBlog PostPublishedNov. 3, 2020, 2:15 p.m....are not typically persuaded to fire when light is shined on them, but the expression of light-gated ion ... (ChRs) makes them light-responsive. When light shines on excitatory channelrhodopsin-expressing neurons... -
Developing Lab Management Software for Biology
TypeBlog PostPublishedAug. 25, 2016, 2:30 p.m....the appropriate stab culture Grow the stab culture Ship the stab culture to the proper location To keep...technicians to retrieve the sample and get it ready for shipping. In the first case, we naively assume that everything... -
Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9
TypeBlog PostPublishedDec. 16, 2015, 3:30 p.m....plasmids from this publication at Addgene. 2. Nishimasu, Hiroshi, et al. "Crystal structure of Cas9 in complex... -
New Tool for Lineage Tracing: The ClonTracer Library
TypeBlog PostPublishedSept. 22, 2015, 2:30 p.m.... research environment” with a strong focus on publishing, but still made it so she could “get exposure...work. She says, “I spend a lot of time packing, shipping, and sharing protocols one by one,” and she is... -
Plasmids 101: The Wide World of Natural Plasmids
TypeBlog PostPublishedOct. 8, 2024, 1:15 p.m....categorization is complex Plasmid classification has shifted over the years, and new classifications are still...organization doesn’t necessarily reflect genetic relationships or plasmid incompatibility. Plasmids may also... -
Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them
TypeBlog PostPublishedMay 4, 2021, 1:15 p.m....over 100 scientific institutions have proposed a shift to recombinant DNA-based antibody technologies (...practical choice. Hybridomas are much tougher to ship, often lack sequence data, and may genetically drift... -
A Guide to Designing a Scientific Poster: Content, Layout, and More
TypeBlog PostPublishedOct. 22, 2019, 12:20 p.m....Poster layout While it’s good to let your ingenuity shine, and you generally have free reign over how you ...catchy, but beware. A distracting background or clashing colors can take away from what should be the focus... -
Pathways Over Time Plasmids Engage Students in Functional Genomics Research
TypeBlog PostPublishedMay 22, 2018, 1:38 p.m....Undergraduate Biology Education: A Call to Action, Washington, DC. 2. President's Council of Advisors on Science...Science, Technology, Engineering and Mathematics, Washington, DC: US Government Office of Science and Technology... -
CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems
TypeBlog PostPublishedJuly 23, 2020, 1:20 p.m.... editing while still allowing on-target editing (Shin et al., 2017). Acrs could also be used to reduce....e9 . https://doi.org/10.1016/j.cell.2016.11.017 Shin J, Jiang F, Liu J-J, Bray NL, Rauch BJ, Baik SH,... -
Corralling and Cornering the Goldilocks Antibody
TypeBlog PostPublishedAug. 9, 2022, 1:15 p.m....strong signal. However, exposure time and its relationship to signal depend on what you’re comparing your...working with Dr. Wu please contact us here! Ava Shipman is a research assistant in lab of Zhuhao Wu at ... -
Cpf1 Update: Comparison to Cas9 and NgAgo
TypeBlog PostPublishedJuly 14, 2016, 2:30 p.m....upstream of the PAM site, Cpf1 cleaves in a staggered fashion, creating a 5 nucleotide 5’ overhang 18-23 bases...publication at Addgene. 4. Makarova KS, Wolf YI, Alkhnbashi OS, Costa F, Shah SA, Saunders SJ, Barrangou ... -
Plasmids 101: FLEx Vectors
TypeBlog PostPublishedApril 28, 2016, 2:30 p.m....cell populations. Acknowledgement:Through a partnership with genOway, we are able to distribute materials...Luo Massimo Scanziani Scott Sternson Roger Tsien Naoshige Uchida Ian Wickersham Hongkui Zeng Additional...