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We narrowed to 587 results for: SIM

Showing: 201 - 220 of 587 results
  1. Activate Addgene's "Find Plasmids" Badge on Pubmed Abstracts

    Type
    Blog Post
    Published
    May 12, 2017, 12:58 p.m.
    ...that within that article you’ll find new plasmids. Simply click on the badge to be taken directly to the ...an account on PubMed. Once you have an account, simply click on your account name in the top right corner...
  2. The Addgene Antibody Data Hub

    Type
    Blog Post
    Published
    Sept. 12, 2024, 1:15 p.m.
    ...application. The submission process is easy, with simple, descriptive fields, drop-down boxes when appropriate...can work through it at your own pace. To submit, simply click "Contribute Data."    Figure 6: Contributing...
  3. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...ladders are not next to empty wells. Most people will simply load an extra ladder or technical replicate (ie... leftover sample) to fill the relevant wells. Similarly, if you load a gel and then forget to start it...blocking time! Fill the time with smaller tasks, or simply enjoy some well-deserved down time while waiting...
  4. How Do I Place an Addgene Order Using a PO Number?

    Type
    Blog Post
    Published
    Feb. 27, 2020, 2:15 p.m.
    ...Addgene, we try to make the process as smooth and simple as possible. We are the Customer Support Specialists... Placing an order on Addgene’s website is quite simple, even if you’re using a PO number. Follow these...
  5. IDP and your PI: A Roadmap for Career Planning and Personal Development

    Type
    Blog Post
    Published
    July 21, 2015, 2:30 p.m.
    ...DePace’s yearly planning meetings revolve around two simple worksheets, each of which is filled out separately...Extracurricular Activities for a Strong Science Career Path 7 Simple Strategies to Resolve Conflict With Difficult Supervisors...
  6. Inside Addgene's 10 Year Anniversary Gala

    Type
    Blog Post
    Published
    July 3, 2014, 3:48 p.m.
    ... it to fruition. The concept, though relatively simple, changed the course of scientific research. What...of requesting and sharing plasmids has become simplified through Addgene. Looking back, Addgene’s journey...
  7. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    Published
    Nov. 19, 2013, 2:37 p.m.
    ...cerevisiae and S. pombe. The protocol is surprisingly simple, requiring only a PCR product containing the modifying...ensure that all desired tags have been integrated. Simply design your amplification primers with the desired...
  8. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    Published
    Dec. 12, 2023, 2:15 p.m.
    ...green fluorescent proteins. nLightR did perform similarly to GRABNE, and having both colors allows for increased...AR. Selectivity Norepinephrine and dopamine are similar molecules, and cross-reactivity is a very common...
  9. Year of the Camelid: Antibody Style

    Type
    Blog Post
    Published
    May 9, 2024, 1:15 p.m.
    ...antibodies in the serum of a camel and soon after found similar antibodies in llamas and alpacas (Figure 1). At...Suitability for in vivo experiments, due to their simple structure    sdAbs in research These advantages...
  10. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    Published
    Nov. 22, 2016, 3:30 p.m.
    ...spent collecting it will have been worthless. But simply writing down your data doesn’t guarantee a publication...welcoming us. Then it was time to work! Set up similarly to the online class, we met as a group for sessions...
  11. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    Published
    June 9, 2016, 2:30 p.m.
    ...problem, Gao et al. searched for Argonaute orthologs similar to TtAgo and PfAgo, another thermophilic Argonaute...variety of mammalian cell lines, with an efficiency similar to that of Cas9. Like Cas9, NgAgo is also able ...
  12. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    Published
    Jan. 22, 2019, 2:41 p.m.
    ...biosensor is the GFP-LC3-RFP-LC3ΔG probe. It’s similar to the mRFP-GFP-LC3 Tandem Fluorescent Protein,...fluorescent protein oligomerization Seeing red: simple GFP photoconversion Light sheet fluorescence microscopy...
  13. Antibodies 101: Epitope Availability

    Type
    Blog Post
    Published
    Jan. 11, 2022, 2 p.m.
    ...target protein? Epitope Availability The answer is simple: epitope availability. An antibody can only bind...that has been validated for an assay that uses similar sample preparation - for instance, if you cannot...
Showing: 201 - 220 of 587 results