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  4. Year of the Camelid: Antibody Style

    Type
    Blog Post
    Published
    May 9, 2024, 1:15 p.m.
    ...antibodies in the serum of a camel and soon after found similar antibodies in llamas and alpacas (Figure 1). At...Suitability for in vivo experiments, due to their simple structure    sdAbs in research These advantages...

  7. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    Published
    Nov. 22, 2016, 3:30 p.m.
    ...spent collecting it will have been worthless. But simply writing down your data doesn’t guarantee a publication...welcoming us. Then it was time to work! Set up similarly to the online class, we met as a group for sessions...

  9. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    Published
    Jan. 22, 2019, 2:41 p.m.
    ...biosensor is the GFP-LC3-RFP-LC3ΔG probe. It’s similar to the mRFP-GFP-LC3 Tandem Fluorescent Protein,...fluorescent protein oligomerization Seeing red: simple GFP photoconversion Light sheet fluorescence microscopy...

  10. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    Published
    March 5, 2020, 2:15 p.m.
    ...e.g. titer, injection site, animal strain used). Similar to the goodwill of Addgene plasmid depositors, ...this resource for the community. There are a few simple steps you can take to input your data for sharing...

  11. Antibodies 101: Epitope Availability

    Type
    Blog Post
    Published
    Jan. 11, 2022, 2 p.m.
    ...target protein? Epitope Availability The answer is simple: epitope availability. An antibody can only bind...that has been validated for an assay that uses similar sample preparation - for instance, if you cannot...

  12. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    Published
    Sept. 21, 2021, 1:15 p.m.
    ... useful natural materials, such as antibiotics. Simon Moore and Paul Freemont’s lab previously developed..., this cell free system allows for a relatively simple, controlled “one-pot” reaction capable of reproducing...

  13. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    Published
    April 25, 2023, 1:15 p.m.
    ...probed simultaneously on the sample. What to do?  If you’ve ever found yourself facing a similar problem...

  14. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    Published
    June 9, 2016, 2:30 p.m.
    ...problem, Gao et al. searched for Argonaute orthologs similar to TtAgo and PfAgo, another thermophilic Argonaute...variety of mammalian cell lines, with an efficiency similar to that of Cas9. Like Cas9, NgAgo is also able ...

  15. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    Published
    Aug. 21, 2014, 3:15 p.m.
    ... out more than necessary for my interview. The simplest place I could think of to start was “Marketing...about marketing would be to start reading a broad simple overview, learn some jargon and key phrases and...

  16. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    Published
    May 6, 2014, 1:07 p.m.
    ...Merkx of Technische Universiteit Eindhoven has similar interest in FRET-based sensor proteins, particularly...monitoring calcium versus zinc should require the simple swapping of binding domains, in practice optimizing...

  17. The MTA at Addgene

    Type
    Blog Post
    Published
    July 23, 2024, 2:17 p.m.
    ...agreement, read on!  What is the MTA?  An MTA is simply a contract or agreement that states the terms of...clearly communicated. And that’s where MTAs, and similar agreements, shine.  These types of agreements allow...

  18. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    Published
    March 1, 2018, 2:11 p.m.
    ...cell and any individual AAV particle is rare. But simply getting into the cell may not always be enough ... can optimize AAV production. We’ve listed some simple steps you can take below but you should read our...

  19. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    Published
    March 14, 2019, 1:54 p.m.
    ...produced? Purifying exo-AAVs is actually a bit simpler than the classical purification protocol involving...summary, exo-AAVs seem to target cell types with a similar specifically as conventionally produced AAV particles...

  20. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    Published
    Dec. 20, 2018, 1:15 p.m.
    ...feasible, three separate things happened nearly simultaneously: The Wellcome Trust funded a center for mitochondrial... process of good research practice: computer simulations, in vitro research, animal models, and several...
Showing: 201 - 220 of 571 results