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Showing: 201 - 208 of 208 results
  1. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    Published
    March 27, 2018, 1:32 p.m.
    ... PMID: 9537413. PubMed Central PMCID: PMC3010411. 2. Kohli, Manu, et al. "Facile methods for generating...and pAAV-TK-Acceptor) so that they can be easily adapted to edit your gene of choice. These vectors are ...
  2. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...effective recovery of knock-ins, but did result in 1-2 kilobases of additional sequence being introduced.... editing methods, other Cas proteins have been adapted for use in C. elegans. In 2017, the Korswagen lab...doi.org/10.17912/DDVH-BG64 Tzur YB, Friedland AE, Nadarajan S, Church GM, Calarco JA, Colaiácovo MP (2013)...
  3. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ... iRFP713, % Oligomeric state Photo-stabilityc, t1/2, s pKa Brightness in HeLa cells vs. iRFP713, % d ... changes occurring due to protein synthesis, degradation, or reconstitution (reviewed in Shcherbakova ...
  4. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ...Nutr, 129 (2009a): 477–484. PubMed PMID: 10064313. 2. Chapman-Smith, A., & Cronan, J. E. “The enzymatic.... The biotin-avidin interaction has also been adapted for use in immunohistochemistry and antibody labelling...
  5. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ...signal separately for each target.   Figure 2: Examples of simultaneous multiplex IF labeling of...C3 show magnified detail of the box in C. Image adapted from Andrews et al., 2019 under CC-BY 4.0 license...
Showing: 201 - 208 of 208 results