We narrowed to 284 results for: cki

1. Antibodies 101: Choosing the Right Isotype

   Type

   Blog Post

   Published

   April 6, 2023, 3 p.m.

   ...References Lu, Q. L., & Partridge, T. A. (1998). A New Blocking Method for Application of Murine Monoclonal Antibody...

2. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

   Type

   Blog Post

   Published

   May 4, 2021, 1:15 p.m.

   ...improve genetically-encoded research tools, it is shocking that the plasmids and sequences for antibodies...

3. Corralling and Cornering the Goldilocks Antibody

   Type

   Blog Post

   Published

   Aug. 9, 2022, 1:15 p.m.

   ...enable scientists to have an intuitive guide for checking their staining and neuroanatomy without the fear...

4. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

   Type

   Blog Post

   Published

   Dec. 14, 2021, 2:15 p.m.

   ...Charlton J, Mattei AL et al (2018). Genome-wide tracking of dCas9-methyltransferase footprints. Nat Commun...

5. Hot Plasmids - October 2020

   Type

   Blog Post

   Published

   Oct. 13, 2020, 1:15 p.m.

   ... opens the door to in vivo experiments such as tracking the synaptic maturation pattern of hippocampal...

6. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

   Type

   Blog Post

   Published

   Oct. 6, 2020, 1:15 p.m.

   ...activation uses dCas9, a CRISPR protein variant lacking its endonuclease ability, to bind to genes without...

7. A Guide to Designing a Scientific Poster: Content, Layout, and More

   Type

   Blog Post

   Published

   Oct. 22, 2019, 12:20 p.m.

   ...different and potentially more catchy, but it can be trickier to make figures fit nicely into circular shapes...

8. Hot Plasmids February 2024

   Type

   Blog Post

   Published

   Feb. 20, 2024, 2:45 p.m.

   ...was delivered to brain endothelial cells in mice lacking LY6A using AAV1-X1 (Chen et al., 2023); then, when...

9. Antibodies 101: Stripping and Reprobing Western Blots

   Type

   Blog Post

   Published

   Aug. 1, 2024, 1:15 p.m.

   ...ll want to check for the primary antibody by re-blocking your membrane and re-incubating with your secondary...

10. Progress Towards a PAM-Free CRISPR

    Type

    Blog Post

    Published

    March 7, 2024, 2:39 p.m.

    ...at least has looser or different requirements. Luckily, there are some available.   Cas proteins that ...

11. Educational Planarian Tools for Teaching Developmental Biology Techniques

    Type

    Blog Post

    Published

    May 24, 2018, 1:20 p.m.

    ...or their translation disrupted through physical blocking of the translation machinery. In the Sánchez lab...

12. Lab Automation at Addgene

    Type

    Blog Post

    Published

    March 22, 2018, 1:28 p.m.

    ...protocol on the Biomek FxP is pretty simple. By clicking a few buttons you can quickly tell the Biomek ...

13. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type

    Blog Post

    Published

    March 8, 2017, 3:15 p.m.

    ...process. Figure 1: Begin the deposit process by clicking “Submit Online Deposit”or “Download Deposit Spreadsheet...

14. Tips for Technical Support Calls

    Type

    Blog Post

    Published

    Sept. 20, 2016, 2:39 p.m.

    ...customers within 24 hours, and we’re pretty good about sticking to that. If you email us at noon and then call...

15. Plasmids 101: FLEx Vectors

    Type

    Blog Post

    Published

    April 28, 2016, 2:30 p.m.

    ... 2003 PubMed PMID: 12665802. 2. Branda, CS and Dymecki, SM. Talking about a Revolution: The Impact of ...

16. Tips for Titering Your Lentiviral Preps

    Type

    Blog Post

    Published

    March 15, 2016, 2:30 p.m.

    ...original plasmid which can over-inflate results. A packing vector deficient in reverse transcriptase will ...

17. Gaining Leadership Skills Volunteering at a Professional Organization

    Type

    Blog Post

    Published

    Aug. 6, 2015, 2:30 p.m.

    ...resulted in actionable tasks and I made a point of sticking to deadlines and performing the work I agreed ...

18. How-to: 5 Steps to a Great Panel Discussion

    Type

    Blog Post

    Published

    Oct. 22, 2014, 1:57 p.m.

    ...audience – phones come out, postures shift, they are checking out. If you can, plant 1-2 good questions with...

19. Of Myc and Men

    Type

    Blog Post

    Published

    Jan. 19, 2023, 2:15 p.m.

    ...involve taking the Myc1-9E10 variable regions and sticking them onto different scaffolds. But over the years...

20. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type

    Blog Post

    Published

    April 4, 2019, 12:53 p.m.

    ...gene in the recipient's genome. Antibiotic plates lacking DAP select against donor bacteria and for recipients...