We narrowed to 247 results for: construct

...background signal (especially for some older split-GFP constructs or certain cell environments). Also consider ...

...vectors and DNA modules for assembly of CRISPR/Cas9 constructs. zCas9i is a high efficiency intron-optimized...

...PGK promoter) and U6 promoter-driven guide RNA constructs, pAdSh.U6.gRNAS1 and pAdSh.U6.gRNAGFP, and also...

...  A second application for CAPTURE 2.0 is the construction of spatial and hierarchical chromatin interaction...

...team characterized and deposited a variety of constructs for visualizing cellular components (nucleus,...

...plasmids — your transfer plasmid and the baculovirus construct, called a bacmid. Like AAV and lentiviral packaging...

...determine recombination rates in FLEX/DIO/fDIO constructs. Endotoxin Endotoxin contamination in vector ...

...specifically isolate bacteria containing your constructs of interest. But does it matter which antibiotic...

... Lohman, G. J. S. (2022). Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly...

...competing models. Comparing model A vs. B is more constructive than testing the validity of a single model....

...allowing the screen to be conducted with only two constructs (the other containing dCas9-VP16). Like CRISPRi...

... different genes without having to create new constructs. However, due to problems such as mismatched ...

...spent two years testing and reformulating our AAV construct to optimize performance in our system and yet ...

...time a new process was added, we progressively constructed the entire workflow in the Nextflow script. With...

...reverse transcriptase, protease, and Bst polymerase constructs from their assay to Addgene.  Drew Endy’s and...

... Figure 2: (A) Streamlined VchINT expression constructs. (B) High integration efficiencies achieved with...

...A206K mutation [2]. Addgene has many of these constructs and they are designated mEGFP, mVenus, etc. A...

...deciding where to place an FP within a fusion construct [1,2], i.e. positioning of the FP sequence relative...

...same multicistronic vector, effectively making a construct that expresses multiple unique genes at equivalent...

...commonly employed in lentiviral and retroviral constructs and we will discuss these in a future post on...