We narrowed to 245 results for: construct

1. Viral Production at Addgene

   Type

   Blog Post

   Published

   Oct. 24, 2023, 1:15 p.m.

   ...determine recombination rates in FLEX/DIO/fDIO constructs. Endotoxin Endotoxin contamination in vector ...

2. Plasmids 101: Choosing an Antibiotic Resistance Gene

   Type

   Blog Post

   Published

   Oct. 10, 2023, 1 p.m.

   ...specifically isolate bacteria containing your constructs of interest. But does it matter which antibiotic...

3. High Complexity Golden Gate Assembly with NEB’s DAD

   Type

   Blog Post

   Published

   Aug. 1, 2023, 1:15 p.m.

   ... Lohman, G. J. S. (2022). Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly...

4. Negative Can Be Positive: Open AAV Data with Addgene

   Type

   Blog Post

   Published

   Sept. 6, 2022, 1:15 p.m.

   ...spent two years testing and reformulating our AAV construct to optimize performance in our system and yet ...

5. Bioinformatics at Addgene

   Type

   Blog Post

   Published

   July 14, 2022, 1:15 p.m.

   ...time a new process was added, we progressively constructed the entire workflow in the Nextflow script. With...

6. Split Fluorescent Proteins for Studying Protein-Protein Interactions

   Type

   Blog Post

   Published

   May 3, 2022, 1:15 p.m.

   ...background signal (especially for some older split-GFP constructs or certain cell environments). Also consider ...

7. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

   Type

   Blog Post

   Published

   July 12, 2021, 1:15 p.m.

   ...reverse transcriptase, protease, and Bst polymerase constructs from their assay to Addgene.  Drew Endy’s and...

8. Hot Plasmids: Summer 2025

   Type

   Blog Post

   Published

   Aug. 28, 2025, 1:15 p.m.

   ...team characterized and deposited a variety of constructs for visualizing cellular components (nucleus,...

9. Production of Virus in Insect Versus Mammalian Cells

   Type

   Blog Post

   Published

   Nov. 5, 2024, 8:32 p.m.

   ...plasmids — your transfer plasmid and the baculovirus construct, called a bacmid. Like AAV and lentiviral packaging...

10. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type

    Blog Post

    Published

    July 2, 2020, 12:40 p.m.

    ...  A second application for CAPTURE 2.0 is the construction of spatial and hierarchical chromatin interaction...

11. Designing Your Chalk Talk for the Academic Job Interview

    Type

    Blog Post

    Published

    March 12, 2019, 1:37 p.m.

    ...competing models. Comparing model A vs. B is more constructive than testing the validity of a single model....

12. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type

    Blog Post

    Published

    Oct. 4, 2018, 12:44 p.m.

    ...allowing the screen to be conducted with only two constructs (the other containing dCas9-VP16). Like CRISPRi...

13. Plasmids 101: Codon usage bias

    Type

    Blog Post

    Published

    Sept. 27, 2018, 1:09 p.m.

    ... different genes without having to create new constructs. However, due to problems such as mismatched ...

14. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type

    Blog Post

    Published

    Dec. 7, 2020, 2:53 p.m.

    ... Figure 2: (A) Streamlined VchINT expression constructs. (B) High integration efficiencies achieved with...

15. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

    Type

    Blog Post

    Published

    April 19, 2016, 2:30 p.m.

    ...A206K mutation [2]. Addgene has many of these constructs and they are designated mEGFP, mVenus, etc. A...

16. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type

    Blog Post

    Published

    Oct. 27, 2015, 3 p.m.

    ...deciding where to place an FP within a fusion construct [1,2], i.e. positioning of the FP sequence relative...

17. Plasmids 101: Multicistronic Vectors

    Type

    Blog Post

    Published

    Sept. 9, 2014, 8:20 p.m.

    ...same multicistronic vector, effectively making a construct that expresses multiple unique genes at equivalent...

18. Plasmids 101: The Promoter Region – Let's Go!

    Type

    Blog Post

    Published

    April 3, 2014, 8:05 p.m.

    ...commonly employed in lentiviral and retroviral constructs and we will discuss these in a future post on...

19. Hot Plasmids Spring 2024

    Type

    Blog Post

    Published

    May 23, 2024, 1:15 p.m.

    ...produced 90% accurate integration of multiplexed constructs into target genomes and a 10-fold improvement...

20. Transferable Skills Guide: Conflict Resolution

    Type

    Blog Post

    Published

    Jan. 4, 2022, 2:15 p.m.

    ...comments on your manuscript is a form of (hopefully constructive) conflict. As you perform the experiments to...