We narrowed to 687 results for: eng

...supported this project with funding. Alnylam and New England Biolabs sponsored the capital construction of our... solicited seed money for the new project. New England Biolabs and Alnylam see the value to the scientific...research and discovery, while also pushing us to challenge assumptions, holding us to nonprofit best practices...reach scientists around the world. Our Software Engineering Team (and, added in 2018, our Product Team) continues...

...longstanding challenge. Using shRNA, TALEN, or ZFN to edit immune cells poses challenges such as the inhibition...Salmonella Typhimurium. This work demonstrates engineered gas vesicles as the first reporter genes using...sgRNA insert site and a reporter for tracing the engineered cells in vivo, the non-immunogenic violet-excited...

...below 4.4kB (with the ITRs bringing total genome length to ~4.7kB.  If your insert alone is 4.2 kB and ...serotypes such as AAV9 or AAV8. Very recently an engineered AAV5 capsid was reported for specific and potent...prohibitive problem? A: Re-administration is a challenge for AAV due to the development of neutralizing...neutralizing antibodies in animal models. All of our engineered AAV are presently built on an AAV9 backbone and ...

... Schematic overview of ChIP. Image courtesy of Shengliu via Wikicommons.   Okay, let’s do a quick ...such as sequencing or qPCR. Yay!  Where’s the challenge in that?  So while this method is certainly long...cell type, amount of protein, and desired fragment length for your output assay. A longer sonication time...the sample type and size.) It can be quite the challenge, and take a few rounds of adjustments, to get ...

...professional development, and while it’s not challenging to use, it’s hard to know where to begin. To ...helped her connect to other black scientists and engineers on Twitter, but also led to interviews on Al Jazeera... statistics on what percent of scientists and engineers regularly use the platform, why scientists are...media, but how you use social media to consistently engage with others about your topic(s) of interest. Hopefully...

...but where the kinases are located, their signal strength, and how long their signals last all impact transduction...the signal must be transmitted by intermediate messengers. Optical control of kinase activity can provide...preserved. References 1. Zhou, Xin X., Linlin Z. Fan, Pengpeng Li, Kang Shen, and Michael Z. Lin. "Optical control...

...itself. In their CRISPRainbow paper, Ma et al. engineered gRNA scaffolds containing two hairpin sequences...permit labeling, but a newly expressed standard length gRNA would “switch” the system and induce genome...Multiplexed labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow.” Nat Biotechnol. 34...Addgene. 4. Kiani, Samira, et al. “Cas9 gRNA engineering for genome editing, activation and repression...

...scientists can also study the microbiome by using engineered genetic tools and reporter microbes. Let’s take...take a look at a few of these methods below. Engineering gut microbes in situ While the ability to monitor...References Leonard, Sean P., et al. "Genetic engineering of bee gut microbiome bacteria with a toolkit...PMC4674677. Ronda, Carlotta, et al. "Metagenomic engineering of the mammalian gut microbiome in situ." Nature...

... immense task (it takes, ooh, approximately the length of your average PhD to achieve!) Each field has...there. In every project, time, attention, and/or length restraints mean you will have to pick and choose...cognitive load changed the way I thought about engaging with learners. If something I was communicating...accessible pieces of communications that drive engagement with your target audience. Thinking about cognitive...

...drawbacks of the indirect method, lengthening an often already lengthy process. (And while I have it on...protein of interest is often one of the most challenging components of any immunoimaging assay. Secondary...has introduced new options for increasing signal strength.  For the time being, though, secondary antibodies...

...year (12% in 2018) since 2005. We’re meeting the challenges of DNA distribution to ensure scientists everywhere...happening. The Addgene team will have some fun challenges this year and I’m excited about this, but definitely...documentaries in 3 languages (Japanese, German, and English) and even on 60 Minutes. We are blessed with a ...appearance and functionality are happening and this strengthens our image in the world. Addgene is now as much...

...improvements in genome engineering! References 1. Slaymaker, Ian M., et al. "Rationally engineered Cas9 nucleases...-target specificity of the Cas9 nuclease with engineered variants: eSpCas9, SpCas9-HF1, & HypaCas9. ...Plasmids Browse our CRISPR Guide Pages Find Genome Engineering Resources ...

... preliminary results (Dimitri et al). CRISPR engineering of CAR-T cells is particularly promising because... the first-ever systemic CRISPR therapy. They engineered a CRISPR-Cas construct targeting the diseased...technology is rapidly evolving, and many of the challenges with therapies involve patient safety, effectiveness...2013). Dimitri, A., Herbst, F. & Fraietta, J.A. Engineering the next-generation of CAR T-cells with CRISPR-Cas9...

...tests.  Labs including Jennifer Doudna’s lab and Feng Zheng’s lab had already developed nucleic acid detections...resources References Biering SB,  Sarnik SA, Wang E, Zengel JR, Sathyan V, Nguyenla X, Van Dis E, Catamura ...NV, Ludes-Meyers J, Nguyen AW, Park J, Wang N, Amengor D, Lavinder JJ, Ippolito GC, Maynard JA, Finkelstein...

...outskirts of Guangzhouon west of beautiful Mao Fengshan Forest Park. As a national science and technology...one take dried seahorse for?), but there were no English speakers around. Opening talks – Genomics discoveries... sensitizing gene cassettes. Thse vectors are engineered to replace the drug resistantance genes in the...cas9 proteins from different species for genome engineering. The final keynote presentation was by Nobel ...

... to be prepared to take on the challenges and be proactive and engaged once you are in a leadership role...online and on monthly phone calls. This has its challenges as meeting in person is often much more productive...different types of people in order to harness their strengths and work effectively. Leadership responsibilities...

...this complex, developing Cas3 tools has been challenging. It can be difficult to ensure comparable expression..., though consistent expression can still be a challenge. A multitasking Cas Most Cas effectors have one...Harnessing type I CRISPR–Cas systems for genome engineering in human cells. Nature Biotechnology, 37(12),...CRISPR-Cas Systems INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons Additional resources...

...program itself. Picking a mentor comes with many challenges, and the flood of interview requests and visitation...logistical, financial, etc…) now have options to fully engage in the application process. And even if you intend...a travel day to an online call in response to challenges on their end. Though the quick changes in plans...using the virtual world to your advantage can strengthen your application and help you decide if a program...

...Nowadays a toolbox of naturally occurring and engineered AAV serotypes that further expands the range ...currently used in clinical trials, a variety of engineered ones have shown great promise in preclinical ... AAV safety profile is excellent and further strengthens its position as the vector of choice for in vivo...being done to further improve the technology: engineering of new capsid variants, designing expression ...

...immunodetection tools is their strong signal strength. This signal strength is due in large part to their larger...requires animal sacrifice and precludes genetic engineering of a tag or fusion with a reporter enzyme. For...labeled with up to 3 dyes, yielding similar signal strength to a standard IgG secondary antibody (Pleiner ...