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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 17 results for: eng

Showing: 1 - 17 of 17 results

  1. Protocol - How to Run an Agarose Gel

    Type
    Protocol
    ...lab procedure for separating DNA by size (e.g., length in base pairs) for visualization and purification...longer ones. Thus, you can determine the approximate length of a DNA fragment by running it on an agarose gel...ladder (a collection of DNA fragments of known lengths). Last Update: Feb. 20, 2018 Protocol Video Watch...concentration of 10 mg/mL) Gel loading buffer, New England Biolabs B7022S Procedure Pouring a Standard 1% ... be purifying the DNA for later use, use long-wavelength UV and expose for as little time as possible ...

  2. DNA Quantification

    Type
    Protocol
    ...absorb different wavelengths of light to varying degrees and most have a specific wavelength that they maximally...based on its absorbance, you need to know the wavelength of light that your substance maximally absorbs...

  3. Gibson Assembly Protocol

    Type
    Protocol
    ... (Gibson et al. , 2009). Regardless of fragment length or end compatibility, multiple overlapping DNA ...fragments at a time. Resources and References New England Biolabs (NEB) (Link opens in a new window) sells... window) . Gibson Assembly® is licensed to New England Biolabs by TelesisBio. Gibson DG, Young L, Chuang...

  4. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...used for ligation, use only long-wavelength UV light. Short wavelength UV light will increase the chance...Materials Material Vendor and catalog # AgeI New England Biolabs (NEB) #R0552S EcoRI NEB #R0101S NEB buffer...

  5. Protocol - How to Purify DNA from an Agarose Gel

    Type
    Protocol
    ...gel electrophoresis, which separates DNA by their length in base pairs. Following electrophoresis, you can...cutting out the bands and you will want to use long-wavelength UV for as short a time as possible to get the...

  6. Ligation Independent Cloning

    Type
    Protocol
    ... DNA polymerase can create overhangs of varying length (typically 10-12 bp) based on a specific sequence...or tag sequences (where appropriate). The primer length is dependent on the T4 Pol "chew back" reaction...

  7. Protocol - How to Ligate Plasmid DNA

    Type
    Protocol
    ...the amount of DNA used in a ligation based on the length of the DNA to get a proper ratio of 3 available...found on the web. Just enter the concentration, lengths of your insert and vector, and what ratio you want...

  8. Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

    Type
    Protocol
    ...cells. The problem is that the only version of full-length cDNA you can find for YGOI is in a bacterial expression...enzyme buffer (see (Link opens in a new window) New England Biolabs for more information about restriction ...

  16. AAV Production in HEK293 Cells

    Type
    Protocol
    ... until fully dissolved. Pro-Tip This step is challenging due to the high viscosity of PEG. Stirring under...

  17. CRISPR Library Amplification

    Type
    Protocol
    ...Quality Control (QC) Pooled libraries can be challenging and expensive to ensure adequate quality, but...
Showing: 1 - 17 of 17 results