Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 533 results for: erf

Showing: 201 - 220 of 533 results

  1. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    Published
    Aug. 29, 2024, 1:15 p.m.
    ... a destination vector via Golden Gate cloning, perform an exonuclease cleanup, transform, plate, and pick...sequencing and found 98% of the plasmids were perfectly correct. Because they created the assemblies in...

  2. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    Published
    July 18, 2019, 12:55 p.m.
    ...embryonic genes are individually depleted via RNA interference (RNAi). Scientists monitor the effects of RNAi...RNAi using four-dimensional contrast interference (DIC) microscopy and computer imaging processing. The...

  3. How to Prepare for an Industry Interview

    Type
    Blog Post
    Published
    July 9, 2020, 1:15 p.m.
    ...general questions about job responsibilities, performance evaluations, professional development, and company... research is a deliverable. Find 10 steps to a perfect science talk Be prepared for potential technical...

  4. Engaging with science and society at pgEd

    Type
    Blog Post
    Published
    Sept. 20, 2018, 12:17 p.m.
    ...policy requires broad interest in issues at the interface of science and society, and an ability to talk...in the legal, ethical and policy issues at the interface of biology and society.     Additional resources...

  5. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    Published
    July 24, 2018, 12:51 p.m.
    ...expressing RNAi pMKO.1 puro (Plasmid #8452) RNA interference (RNAi) is an RNA-mediated gene silencing mechanism...be combined with high-throughput sequencing to perform multiplexed analysis of cells pre-treated with ...

  6. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    ...their genes of interest. While recombinases are powerful gene expression tools in and of themselves, their...reduced scale in screens without any reduction in performance compared to larger genome-wide libraries. Find...

  7. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    Published
    Aug. 9, 2022, 1:15 p.m.
    ...non-target proteins), variability (separate batches can perform differently – happens when the antibody is produced...binding to off-target molecules), reliable (wouldn’t perform differently over time), compatible with the desired...

  8. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...quickly saw wide interest, its dimeric nature can interfere with some applications. Several labs have been... (Chuapoco et al., 2023). They found the best-performing variant, CAP-Mac, was effective at breaching ...

  9. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ...’t common to non-scientists and may make your perfectly reasonable paragraph unintelligible to a lay audience...Science Communication Skills 5. Practice makes perfect, apply for small grants too There are actually ...

  10. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ...target sites that flox your genes of interest, a powerful FLEx switch can be created for your specifc experimental...more information. References: 1. Schnütgen F, Doerflinger N, Calléja C, Wendling O, Chambon P, and Ghyselinck...

  12. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    Published
    Feb. 11, 2025, 2:15 p.m.
    ...through Cas3. After these initial steps, Cas3 can perform its helicase and nuclease functions simultaneously...likes to bluff, making you into think it’s more powerful than it is. The nuclease domain of Cas3 is considered...

  13. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ...allows for a wide variety of screens, including interference, activation, and knockout. Most CRISPR screens...CRISPR Delivery Genome-wide Screening Using CRISPR Performing In Vivo CRISPR Screens Using the FITS Approach...

  14. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ...just be an artifact and eliminate sources of interference, but the result persisted. (Even better, multiple...Although a ~1% change in signal might not make a very powerful tool, it was promising enough to keep exploring...

  15. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    Published
    March 28, 2018, 6:52 p.m.
    ... NGA is a secondary PAM for SpCas9. Hu et al. performed GUIDE-seq analysis to characterize genome-wide...an NGG PAM, xCas9 3.7-VPR constructs modestly outperformed SpCas9-VPR in activating transcription. Sites...

  16. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...student at the Max Planck Institute of Colloids and Interfaces. Here are some tips for cell culture that will...lab at the Max Planck Institute of Colloids and Interfaces. Sana is particularly interested in Glycobiology...

  17. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ...protein. The Bender’s popularity was short-lived as powerful molecular graphics packages were soon developed...channel.   He then said “I hadn’t realized how perfectly flat the complex is on this surface”….where the...

  18. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ...have its limitations, especially when trying to perform high-throughput experiments. If a user wants to... modified to tag hundreds of genes, generating powerful genetic screening libraries. Additional protein...

  19. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    Published
    April 4, 2019, 12:53 p.m.
    ...them. For example, gene knockdown with CRISPR interference (CRISPRi) has been limited to lab-adapted bacteria... Resources on Addgene.org Browse our CRISPR interference plasmids Find bacterial CRISPR plasmids Read...

  20. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...every work setting and no manager will ever be perfect, but the chances of getting a good manager do tend...at once. Many companies will hire a postdoc to perform a specific project, while others will hire a group...
Showing: 201 - 220 of 533 results