We narrowed to 291 results for: fus
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TypeBlog PostPublishedDec. 21, 2017, 2:06 p.m....coding sequence feature that may be used to make fusion genes, detection needs to occur even if one or ...
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10 Great Guest Posts We're Thankful For
TypeBlog PostPublishedNov. 18, 2016, 3 p.m....that respond to different pH’s and some that are fused to fluorescent proteins. She also showed that R-... -
Plasmid Cloning by PCR
TypeBlog PostPublishedMarch 29, 2016, 2:30 p.m....in the recipient plasmid by viewing the MCS and fusing the upstream restriction site to the forward primer... -
FPbase: A new community-editable fluorescent protein database
TypeBlog PostPublishedMay 16, 2018, 1 p.m....environmental conditions (such as temperature, pH, fusion protein, etc...). There are many online guides... -
Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System
TypeBlog PostPublishedJune 4, 2019, 1:02 p.m....abundance of a protein variant library is monitored by fusing the variants to eGFP followed by an internal ribosomal... -
FlipGFP, a novel fluorescence protease reporter to study apoptosis
TypeBlog PostPublishedMay 21, 2019, 12:10 p.m....collection Find empty backbones for fluorescent protein fusions Browse the fluorescent proteins plasmid kits ... -
The Crispr Babies Saga Shows the Need for Action, Not More Delays
TypeBlog PostPublishedDec. 20, 2018, 1:15 p.m....incorrectly known as “three-parent babies,” where fused genetic material from intended parents is “rehoused... -
Summer SciComm Series: A PhD in Science Communication
TypeBlog PostPublishedAug. 23, 2022, 1:15 p.m.... for advice on her baby’s health but adamantly refused to get them vaccinated. A classmate told me that... -
The MTA at Addgene
TypeBlog PostPublishedJuly 23, 2024, 2:17 p.m..... Why the MTA? The MTA process can be a bit confusing, and it’s certainly frustrating to find out your... -
Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish
TypeBlog PostPublishedNov. 27, 2018, 2 p.m....as wild lettuce. Aside from their use as food, infusions of L. taraxacifolia leaves are widely used to ... -
R Bodies: Membrane-Rupturing Microscopic Tools
TypeBlog PostPublishedApril 14, 2016, 2:30 p.m.... functional. Attaching the fluorescent protein fusions allowed us to image R bodies inside cells, and ... -
A Practical Approach to Choosing the B(right)est Fluorescent Protein
TypeBlog PostPublishedJune 1, 2017, 2:30 p.m....with a plasmid containing a fluorescent protein (fusion) there is huge variation in the fluorescence intensities... -
Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection
TypeBlog PostPublishedMay 11, 2017, 2:30 p.m....clarity. But, often these methods don’t agree. Confusing indeed! We've developed a new plasmid toolkit,... -
Top Requested Lentivirus and AAV of 2016
TypeBlog PostPublishedJan. 6, 2017, 3:56 p.m....human muscarinic receptor 4 (Gαi-coupled) DREADD fused to mCherry, all under the control of the human synapsin... -
Why and how to start studying chromatin (with Addgene's help)
TypeBlog PostPublishedJune 12, 2018, 11:40 a.m....Cell. 2009 Mar 27;33(6):717-26. Reporter-nanobody fusions (RANbodies) as versatile, small, sensitive immunohistochemical... -
Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering
TypeBlog PostPublishedMay 3, 2018, 1:48 p.m.... al. saw great results with CasRx and AdmCas13d fused to a nuclear localization signal (NLS). In a HEK293... -
Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME
TypeBlog PostPublishedJune 6, 2019, 12:41 p.m....buoyancy. These proteins allow dissolved gases to diffuse in and out but prohibit water from entering their... -
Antibodies 101: ChIP
TypeBlog PostPublishedMarch 10, 2022, 3:25 p.m....molecular and cell biology techniques. What’s all the fuss? Well, ChIP’s complexities come not from the techniques... -
Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors
TypeBlog PostPublishedMarch 17, 2020, 1:32 p.m....catalytically impaired Cas protein (dCas or Cas nickase) fused to a DNA-modifying enzyme, in this case a deaminase... -
Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs
TypeBlog PostPublishedJune 2, 2015, 6:06 p.m....systems with limited packaging capacity. Still confused about the various tRNA expression strategies? ...