We narrowed to 513 results for: gats

...Crystal Shih is an Investigator III at Novartis, a multinational pharmaceutical company based in Switzerland...collaborative nature of working in industry and how delegating can empower people Chapter 3: How Crystal advanced...

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...in human cells They used the Golden Gate cloning method to ligate expression cassettes of the gRNAs into...

...expressed well in cells and did not form undesirable aggregates. Using budding yeast as a model system, Georgieva...overexpression, while the rest of the fusions had some negative effect on these parameters. IT5 and IT6 displayed...

...developed and implemented by applying the Golden Gate cloning method to human proteins known as Pumilio...lose their solubility. Her lab is currently investigating the issue and hopes to have a better understanding...

...Super 8x TOPFlash, scramble shRNA for use as a negative control, and empty backbones such as pBABE-puro... Our most popular kit of the year was the Golden Gate TALEN Kit, which continues to be our most requested...

...weekend in the United States, I look forward to gathering around a large table for a meal with my family...thankful for many things, and one is that I get to gather around a large table to eat with my Addgene family...

... in cancer Previously available methods for investigating and functionally validating the thousands of...useful for? They could be used to more thoroughly investigate any of the dozens of wild-type genes and their...

... defined contexts would be quite useful for interrogating these scenarios. X-CHIME We recently developed...To demonstrate the power of these systems to investigate in vivo mechanisms, we targeted two phosphatases...

...have been enchanting people for years and have gathered much acclaim - including being featured in the...smaller competitions are now taking place and gathering momentum, including in Cambridge and Bristol. ...

...resources this way, the CCRP2 is giving Rutgers investigators the agility to rapidly initiate complex research... As researchers around the world continue to investigate COVID-19, and once universities reopen and restart...

...particularly encourage people to upload and share their negative data. After all, they tell me, when something ...blog Antibodies 101: Multiplex Immunofluorescence Negative Can Be Positive: Open AAV Data with Addgene Antibodies...

...for students, postdocs, and young Principal Investigators (PIs). We've interviewed Addgene depositors ...Always be sure to include various positive and negative controls to “convince [any] old skeptic professor...

...cloning methods (restriction enzyme, ligation independent, Gateway, Gibson, etc). The cloning method is...

...biomedical research community, but also allows cross-interrogation of different datasets that might otherwise be...to identify. Figure 2: Users can cross-interrogate screens from the literature for a drug, disease...

...thermophiles (high OR medium heat loving), but not obligate thermophiles. However, the recent discovery of... CRISPR/Cas9 genome editing in facultative and obligate thermophiles without the need to grow the organisms...

...both in brain slices and freely behaving mice to gather the following types of data: Report time-course...and mouse brain slices and freely moving mice to gather the following types of data: Report endogenous...

...created a collection of plasmids called TRUPATH to interrogate 14 of the 16 non-visual G protein signal transducers...TRUPATH, an open-source biosensor platform for interrogating the GPCR transducerome. Nat Chem Biol 16:841...

...including replications and experiments resulting in “negative data” often dominate the early years of a project...recommend sharing ReFigures on your Linkedin and ResearchGate profile. To see how to create ReFigures in a...

...stayed to see the Opening Keynote address with Bill Gates. The long line for bag check was definitely worth...for global health and the various initiatives the Gates’ Foundation is involved with was one of the highlights...