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We narrowed to 411 results for: genome

Showing: 201 - 220 of 411 results

  1. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    Published
    Aug. 1, 2017, 1:15 p.m.
    ...subsequently sequenced and aligned to the genome to determine genome-wide translational frequency. Although...states. References 1. Ingolia, Nicholas T., et al. “Genome-wide analysis in vivo of translation with nucleotide...

  2. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type
    Blog Post
    Published
    June 2, 2020, 1:15 p.m.
    ...plasmid deposit please sign up here.  Human whole genome sgRNA iBAR library Article contributed by Susanna...library covers every annotated human gene, it enables genome-wide screening with a high MOI, which is beneficial...settings. Find the sgRNAiBAR library!  Zhu et al., Genome Biology, 2019. PubMed PMID: 30678704. Sparse cell...

  3. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...stably integrates transfer DNA (tDNA) into the genomes of the plants it attacks Growing the transformed...stress of selection you can clip a leaf and extract genomic DNA for genotyping. This won’t hurt your plant ...PMCID: PMC1636043. 3. Kyndt, T. et al. (2015) ‘The genome of cultivated sweet potato contains Agrobacterium...resources on the Addgene blog Engineering the plant genome using CRISPR/Cas9 Using pSiM24 for simplifying ...

  4. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ... cells due to its (random) integration into the genome, and its large DNA packaging capacity is well suited...Additionally, AAVs do not integrate in the host genome but still lead to long-term (>10 years) transgene... Z., 2018. In vivo Applications of CRISPR-Based Genome Editing in the Retina. Front Cell Dev Biol. 2018... Virus (AAV) for cell and gene therapy AAVs for genome editing AAV: A versatile tool for gene expression...

  5. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ...from S. pyogenes (SpCas9), is truly an exceptional genome engineering tool. It is easy to use, functional...CRISPR-associated proteins within the bacterial genome. From this analysis, they identified 53 potential...DNA, identify tumor mutations within cell-free genomic DNA, and detect COVID-19 (Joung, Ladha et al., ...Featured Topic Page Learn about Anti-CRISPRs Multiplex Genome Editing with Cpf1 Resources on Addgene.org Browse...

  6. Addgene’s Top 10 Blog Posts from 2014

    Type
    Blog Post
    Published
    Dec. 16, 2014, 8:01 p.m.
    ...that interest in CRISPR continues to grow as the genome editing technique has developed and improved. The... of 2014 How to Design Your gRNA for CRISPR Genome Editing By John Doench (Guest Blogger) Plasmids...

  7. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ...J. S. (2021). Genome-wide programmable transcriptional memory by CRISPR-based epigenome editing. Cell,...precisely integrating large DNA sequences into a genome (Anzalone et al., 2022). While prime editing and...plasmid containing a complementary site into the genome at this site, resulting in the integrated sequence... autoinhibited methyltransferase DNMT3A to the genomic location specified by the DNA-binding protein. ...in mouse brains for at least six months. Unlike genome editing, this epigenetic strategy doesn’t induce...

  8. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ...Library for Advanced Engineering of Plant Genomes.” The Plant Genome, vol. 16, no. 2, Wiley, 9 Mar. 2023. ...Parts III kit has a focus on transformation and genome engineering. This is a specialized system with ... for the multiplexing of sgRNAs against unique genomic targets or multiple sgRNAs against the same target... Florian, et al. “A Modular Cloning Toolkit for Genome Editing in Plants.” BMC Plant Biology, vol. 20,....15197. Grützner, Ramona, et al. “High-Efficiency Genome Editing in Plants Mediated by a Cas9 Gene Containing...

  9. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    Published
    Dec. 6, 2016, 3:30 p.m.
    ...One huge reason CRISPR has become such a popular genome editing tool is its developers’ willingness to ...this is a particularly prominent problem in CRISPR genome editing experiments. We aim to alleviate this frustration...lentiviruses for delivery of SpCas9 and Cas9 nickase for genome editing. You can also enhance expression of your...

  10. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    Published
    Oct. 19, 2023, 1:15 p.m.
    ...an AAV plasmid an AAV plasmid.  Within the AAV genome, ITRs serve as the origin of replication and are... that flank the ends of the single stranded DNA genome of AAV in the native virus. In an AAV vector plasmid...nicking. Since Rep proteins are important for viral genome replication, these mutations can affect transduction...

  11. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ... is our understanding that AAV-BR1 has the same genome size restrictions as other AAV. Therefore, one ...cargo below 4.4kB (with the ITRs bringing total genome length to ~4.7kB.  If your insert alone is 4.2 ...preclinical researchers have used the flexibility of AAV genome design in remarkably creative ways. The continued...

  13. Editor's Choice, October 2016

    Type
    Blog Post
    Published
    Nov. 4, 2016, 2:30 p.m.
    ...CRISPR in plants, to learn techniques for detecting genome edits, to discover why you might want to use Reddit...Part 2: Building Community Engineering the Plant Genome Using CRISPR/Cas9 Healthcare Consulting: A Door...

  14. A Scientist's Guide to Ebola

    Type
    Blog Post
    Published
    Oct. 16, 2014, 3:01 p.m.
    ...single, linear, negative-sense, single-stranded RNA genomes. The virus does all of its damage with just seven...recommend a few: -The recently established Ebola Genome Portal at the University of California, Santa Cruz...

  15. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ... Although the lab did not conduct comprehensive genome-wide studies of ABE specificity, their other experiments... tightly constrained by PAM availability in the genome. So the development of near-PAMless Cas9 variants...significantly expands the library of accessible genomic targets for base editors (Walton et al., 2020)....2017). Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage. Nature, 551(7681), 464...Zhao, K. T., & Liu, D. R. (2017). Increasing the genome-targeting scope and precision of base editing with...2016). Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage. Nature... N., & Kleinstiver, B. P. (2020). Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9...

  16. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

    Type
    Blog Post
    Published
    Dec. 15, 2016, 3:57 p.m.
    ...alternative method that can be used for cloning or genome engineering and is based on homologous recombination...and will have more unintended changes to their genomes. 2) Designing ssDNA oligos that avoid MMR activation...when introduced into bacteria, integrates into the genome.  Mini-λ uses the endogenous red promoter pL and...stable integration of the phage into the E. coli genome. Selection and confirmation of recombinant clones...in Mammalian Cells CRISPR Methods for Bacterial Genome Engineering Resources on Addgene.org Browse our...

  17. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...integration of multiplexed constructs into target genomes and a 10-fold improvement in detection efficiency...efficiencies in a number of cell types at different genomic loci — in some cases, over 20-fold higher editing...abundant proteins and a critical component for viral genome packaging. Addgene recommends both for use in western.... mRNA trans-splicing dual AAV vectors for (epi)genome editing and gene therapy. Nat Commun., 14(1), 6578...

  18. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    Published
    Aug. 2, 2016, 2:30 p.m.
    ...generally applies to plasmids, but may also facilitate genome editing. Tailored mutations are commonly introduced...induced double-stranded break. This site-directed genome editing requires a template of high homology to...approach can be used to identify Cas9/CRISPR-induced genome modifications. Occasionally, multimerization of...Plasmids 101 Series Use CRISPR to Generate Specific Genomic Mutations Resources on Addgene.org Browse Our...

  20. Grad School Advice Part 2: Building Community

    Type
    Blog Post
    Published
    Oct. 6, 2016, 2:30 p.m.
    ... Ph.D candidate in the Program in Genetics and Genomics at Harvard Medical School. He studies developmental... a graduate of the PhD program in Genetics and Genomics at Harvard Medical School, and is currently a ...
Showing: 201 - 220 of 411 results