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  1. Download Science-Themed Zoom Backgrounds from Addgene

    Type
    Blog Post
    Published
    April 22, 2021, 1:15 p.m.
    ...lentiviral plasmids include popular envelope and packaging plasmids as well as transfer plasmids. Download... the plasmids that Addgene offers by visiting our collections page. Here, you can browse plasmids by field...cute mascots!  Blugene has been helping us make Plasmids 101 videos and needed a break so they're hanging...   Do you do a lot of transformations with the plasmids you receive from Addgene? Our bacteria background...is probably a good fit! Did you know that most plasmids from Addgene are shipped as bacterial stabs? You...

  2. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...absorb the nucleic acids. Containinants bind cellulose less tightly than nucleic acids, so placing the dipstick...contaminants while nucleic acids stick around. Finally, purified total nucleic acids are released by dipping...bacterial cultures or agarose gel slices Product: plasmids or DNA fragments The plasmid purification and ...mammalian cells or tissue Product: total nucleic acids While glass or silica are commonly used to bind ... efficiently binds, or at least entraps nucleic acids. The Botella lab took advantage of this property...pipette.  Here’s how this method works: nucleic acids are broken out of cells with a solution of salts...Additional resources on the Addgene blog Read our Plasmids 101 blog series Find other plasmid protocols and...

  3. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...system consisted of three plasmids: the packaging, envelope, and transfer plasmids. The packaging plasmid... packaging and envelope plasmids are two of Addgene’s most requested plasmids! Lentiviral particles with...genome integration. After co-transfection of these plasmids, 293T cells released transgene-containing lentiviral...recombination between the transfer and packaging plasmids does exist. To further reduce this possibility... First, the packaging system is split into two plasmids: one encoding Rev and one encoding Gag and Pol...transgene-expressing or knockout cell lines. Many CRISPR plasmids are designed for lentiviral use, including Addgene...impact and safety. Many of Addgene’s most requested plasmids are used with lentiviral expression systems. If...

  4. A new platform for fast functional characterization of potentially cancerous mutations

    Type
    Blog Post
    Published
    May 7, 2019, 12:39 p.m.
    ...collection of plasmids each carrying a single gene with a defined mutation or gene fusion. Plasmids carrying...Resources on Addgene.org Find plasmids for your research Find cancer research plasmids and resources ...mutations, and the group has deposited several hundred plasmids from this study with Addgene. Read on to learn...learn more about their work, and how these plasmids can help cancer researchers studying an assortment of...cancer research community, but what else are these plasmids useful for? They could be used to more thoroughly...phenotypic effect in a different system. These plasmids can also help scientists understand how a given...

  5. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ... usage bias in Plasmids 101: Codon usage bias Read more about plasmids in our Plasmids 101 blog series...There are 64 different codons that encode 20 amino acids and three stop codons, meaning that the same amino...prefer certain codons over others for certain amino acids. This is termed codon usage bias. In fact, some ...transfer RNAs (tRNAs). tRNAs bind to and deliver amino acids to the ribosome where they are incorporated into...depicts the 64 codons that encodes the 20 amino acids and three stop codons. (B) Example of codon usage...sequence databases: status for the year 2000. Nucleic Acids Research 28:292–292 . https://doi.org/10.1093/nar...usage bias, and its potential applications. Nucl Acids Res 15:1281–1295 . https://doi.org/10.1093/nar/15.3.1281...

  6. What's New in CRISPR - March 2020

    Type
    Blog Post
    Published
    March 10, 2020, 1:15 p.m.
    ...CRISPR guide Find CRISPR plasmids by function Find recently deposited plasmids ...series, we’ll highlight a few of the new CRISPR plasmids available at Addgene. We will still periodically... Bioengineering Find the optogenetic repressor plasmids Cytosine base editors with minimized off-target...paper in Nature Biotechnology Find the base editor plasmids CRISPRi in Caulobacter crescentus Figure...once. Read the paper in mBio Find the CRISPRi plasmids A multiplexed gRNA piggyBac transposon system... Read the paper in Scientific Reports Find the plasmids Enhanced CRISPR-based DNA demethylation ...paper in Nature Communications Find Casilio-ME plasmids If you have a new CRISPR tool you’ve recently...

  7. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ...Model Organisms blog post Read more about plasmids in our Plasmids 101 blog series Learn more about CRISPR...collection page Find more plasmids and resources for plant resources on Plant Plasmids Collection Page Read...in plasmid manipulation and genetics research. Plasmids are ideal tools for studying genetic modification...disease models.  Check out Addgene’s collection of plasmids containing Rattus norvegicus genes! Mexican axolotl...several vectors for use in the axolotl including plasmids for use in CRISPR and Tol2 transposase induced... and after embryogenesis. Addgene has over 600 plasmids that contain Xenopus genes and a tool-kit from...Read our molecular biology reference Find plasmids for your research ...

  8. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    Published
    Oct. 11, 2016, 2:30 p.m.
    ...for creating plant expression plasmids, including plant CRISPR plasmids from the Yiping Qi lab and the...plant-specific modifications to commonly used CRISPR plasmids are necessary to use the CRISPR system in plant...et al., 2013). Addgene carries Cas9-containing plasmids for knockout, activation and repression of target...systems. Luckily, you don’t have to look far for plasmids that have the plant-specific modifications required...targeting your favorite plant gene; you can find many plasmids for a wide variety of CRISPR applications in plants... available through Addgene. In addition to the plasmids described above, Addgene carries several useful...Plant Parts Kit from the Patron Lab.  As with all plasmids in the repository, we highly recommend reading...

  9. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
    Published
    Sept. 14, 2021, 1:15 p.m.
    ...vectors (LTRs and ITRs), plasmids with multiple polyA sites, and other plasmids that contain repeating ...I help troubleshoot involves viral vectors and plasmids with repeating sequences. Repeating sequences ...other very much undergo recombination, two smaller plasmids are formed. Viral vectors are prone to recombination...and 3’ LTR regions, it splits into two smaller plasmids. The ~3.7 kb recombined vector backbone contains...elements. Knowing how to amplify recombination-prone plasmids and testing your prep can save you the headache...browse the table of common gene mutations in our Plasmids 101: Common Lab E. coli Strains blog post. Check...copy number, try following protocols for low copy plasmids. For example, using a longer growth period (up...

  10. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    Published
    April 25, 2019, 3:01 p.m.
    ...Michael Davidson fluorescent protein collection Check out Addgene’s fluorescent protein plasmids and resources...Biotechnology which allows researchers to deposit plasmids containing the fluorescent protein mNeonGreen....with other fluorescent proteins. Find mNeonGreen plasmids here! mTFP1: a monomeric teal fluorescent protein...physiologically relevant changes in pH. Find mTFP1 plasmids here! mWasabi: a bright green fluorescent protein...excite at 400 nm while EGFP does. Find mWasabi plasmids here! One of the most notable things about these...are best suited for certain studies. If you have plasmids containing mNeonGreen, mTFP1, or mWasabi that ...with the scientific community, please deposit the plasmids at Addgene! References Ai, Hui-wang, et al. "...

  11. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    Published
    Nov. 27, 2018, 2 p.m.
    ...to provide scientific resources, equipment, and plasmids to researchers in resource-limited settings. More...the Addgene-Seeding Labs plasmid grant provide plasmids to these researchers. In the third round of the...biofilms formed on various structured surfaces. Plasmids expressing fluorescent proteins will be used to...microscopy and FACS. In an additional approach, plasmids such as pACBSR-hyg and pFLP-hyg will be used to...regulation and diabetes will be expressed from plasmids (find more information on bacterial expression...information processing during this condition is crucial. Plasmids expressing fluorescent proteins and a new two-...create this bacterial sensor, he will construct plasmids containing promoter regions that activate fluorescent...

  12. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    Published
    June 15, 2023, 1:15 p.m.
    ...which non-canonical amino acids (ncAAs, also sometimes called unnatural amino acids) are placed in a protein...probably quite interested in the canonical 20 amino acids. But in your quest to learn more about your protein...protein of interest, you may find the available amino acids a bit…limiting. It may be time, then, to look towards...and synthetase introduced to the bacteria using plasmids. Rapid expansion occurred, with hundreds of new...Blue Flame award to identify highly requested GCE plasmids. But they’d also love scientist interested in,...and resources, and then checking out all of the plasmids and bacterial strains (available at Addgene for... Site-specific Incorporation of Unnatural Amino Acids into Proteins. Science, 244(4901), 182–188. https...

  13. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type
    Blog Post
    Published
    Sept. 4, 2018, 12:53 p.m.
    ...stationary phase, the number of plasmids per cell increases 4-5 fold (for pUC plasmids) or ~2 fold (for p15a, ...Researchers express genes of interest from plasmids in order to study gene function or to engineer cells...molecular biologist to do? Let’s take a look at how plasmids recently deposited by Chris Voigt’s lab help keep...gene expression and plasmid number. In a suite of plasmids modified from pSC101 with copy numbers ranging...Because pSC101 is not representative of all types of plasmids, the stabilized promoters were also tested in ..., R6K, and ColE2 plasmids) as cell division slows (Kittleson et al., 2011). Segall-Shapiro et al. showed...collections page Download the Plasmid 101 eBook See all plasmids deposited by the Voigt lab ...

  14. All in a Twist: dsRNA

    Type
    Blog Post
    Published
    Feb. 2, 2023, 2:15 p.m.
    ...suffice. This can result in DNA:RNA hybrids. Unlike dsRNA, DNA:RNA hybrids are abundant in normal cells and...when using these reagents. RNA:DNA hybrids Persistent RNA:DNA hybrids that arise during transcription are...Endogenous sources of duplexed RNA DNA:RNA hybrids Sometimes ssRNA just needs a buddy, and even DNA...throughout a single cell, so these nucleic acid hybrids are always around. R-loops are particularly enriched...substrates and in distinct cell cycles. When RNA:DNA hybrids levels are allowed to reach very high or low levels...al., Human proteins that interact with RNA/DNA hybrids. Genome Res., 28(9): 1405-1414 (2018). 10.1101/...

  15. Ghanaian Researchers to Receive Plasmid Awards from Addgene and Seeding Labs

    Type
    Blog Post
    Published
    June 15, 2016, 1:30 p.m.
    ...construction of new plasmids. Dr. Duedu will also use polymerases produced using plasmids from Addgene to ...doctors Bengyella and Duedu and how they plan to use plasmids from Addgene to advance their research. Louis ...cellulosic biomass. Dr. Duedu would like to use the plasmids he acquires through Addgene to produce molecular...fluorescent proteins. Dr. Duedu’s plans for these plasmids have a heavy focus on teaching. He hopes to teach...in environmental samples via PCR. We hope these plasmids will help Dr. Duedu inspire new Ghanaian scientists...and creative ideas applicants will propose for plasmids from the Addgene community and hope we can do ...

  16. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

    Type
    Blog Post
    Published
    March 17, 2016, 2:30 p.m.
    ...separate (and thus smaller) plasmids. Finally, having four separate plasmids allows researchers to partially... four viral genes are each encoded on separate plasmids and are co-transfected into cells alongside a ... the same plasmid (as opposed to four separate plasmids), there are a few reasons arguing against this...Technical tips It is important that all of these plasmids (those containing NP, VP35, VP30, L, and the minigenome...extremely important. For example, particular ratios of plasmids encoding NP and VP35 allow for efficient polymerase... It is important to note that this system uses plasmids where the genes (either viral genes or minigenome...Resources on Addgene.org Browse Our Viral Vector Plasmids Read Our Lentiviral Vector Guide Read Our AAV ...

  17. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    Published
    Dec. 2, 2014, 7:23 p.m.
    ...enChIP Plasmids? All Hodaka Fujii lab plasmids Sign up to receive email alerts when new plasmids from ... shopping for a wide range of plasmids. The merit of depositing plasmids to Addgene has been spectacular... researchers all over the world have requested plasmids for enChIP through Addgene. Retroviral Expression...user-friendly and precise. We will deposit the plasmids of these systems to Addgene in the near future...Community Addgene: In 2012 your lab first deposited plasmids with Addgene. In 2014, you joined Addgene's Advisory...

  18. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    Published
    May 15, 2014, 3:33 p.m.
    ...GFP is a ~27 kDa protein consisting of 238 amino acids derived from the crystal jellyfish Aequorea victoria...from a maturation reaction of three specific amino acids at the center of the protein (Ser65, Tyr66, and .... Scientists can easily utilize GFP-containing plasmids as a means to many functional ends. We’ve listed...each protein or domain being tested. Find FRET plasmids here. Split EGFP: An alternative to FRET, split... marking/selection: Expression constructs like plasmids often include GFP as a marker to help identify...serve as an alternative to antibiotic selection. Plasmids of this type may have the GFP under the control...techniques in fluorescent microscopy. Read More: All Plasmids 101 Articles Addgene's Fluorescent Protein Guide...

  19. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    Published
    Sept. 16, 2020, 1 p.m.
    ...amino acids in size, which includes SpCas9, and shortern orthologs approximately 1000 amino acids in size... Staphylococcus aureus Cas9 (SaCas9, 1053 amino acids) displayed cleavage activity in mammalian cells....small enough to package into AAV. At 984 amino acids in length, Cas9 from Campylobacter jejuni (CjCas9...Neisseria meningitidis Cas9 (NmeCas9) at 1,082 amino acids, can also be packaged into AAV. NmeCas9 has the ... proteins with AAV, at just 1,108 and 986 amino acids, respectively, the size of both is within AAV’s ...capabilities of full-length SpCas9. This set of plasmids includes AAV-Cas9C-VPR for targeted gene activation...Addgene.org Check out our CRISPR Guide Find CRISPR Plasmids ...

  20. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type
    Blog Post
    Published
    July 8, 2025, 1:15 p.m.
    ...blog Plasmids 101: Modular Cloning Plasmids 101: Modular Cloning Applications and Kits Plasmids 101: ...insert’ refers to plasmids carrying each part, ‘vector’ refers to acceptor plasmids, and the ‘amount of...avoid cross-contamination, we recommend retrieving plasmids from your original Addgene kit plate as few times...you will also likely need to miniprep a lot of plasmids from your bacterial stocks beforehand. You can...efficient and precise, positive colonies may contain plasmids with undesired ligations, so always screen multiple...
Showing: 201 - 220 of 678 results