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Showing: 201 - 220 of 455 results

  1. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    Published
    July 16, 2020, 1:15 p.m.
    ... placing orders per day across regions. Our investigation of model fit revealed some potential instabilities...University; and Howard Hughes Medical Institute Investigator. Liu’s research integrates chemistry and evolution...

  2. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    Published
    Feb. 9, 2023, 2:15 p.m.
    ...ownership of their work and become more actively involved in the learning process. As trainees become more... and effort and/or perceived professional risk involved in setting up a CURE, especially if it’s a new...

  3. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ... using droplet digital PCR Droplet digital PCR involves partitioning a PCR reaction mixture into approximately... Witte, Anna Kristina, et al. "A systematic investigation of parameters influencing droplet rain in the...

  4. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ... in using the 3rd-generation system is that it involves transfecting four different plasmids into the ...quickly check that a construct is functional before investing time and resources into making a stable cell line...

  5. Lab to Office Culture Shock

    Type
    Blog Post
    Published
    Feb. 5, 2019, 1:04 p.m.
    ...culture shock? Working in an academic research lab involves a few key features that can be difficult to replicate...sometimes wish for a stretch of time that doesn’t involve critical thinking. Former Addgenie Jessica Welch...

  6. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ... Perhaps you, a colleague, or your adviser was invited to write on a particular topic. Alternatively, ...review articles Whether submitting a review by invitation or by your own accord,  once you have these rules...

  7. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ...resources, time, and experimental needs. Since its invention in the late 1990s, Gateway cloning technology ...technology The Gateway cloning method, developed by Invitrogen, is an in vitro version of the integration and...

  8. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ...resistance testing is requested clinically, this involves growing a standardized amount of the bacteria ...focus on creating new antibiotic therapies, and investigate more effective drug targets (ones that are less...

  9. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    Published
    May 8, 2018, 1 p.m.
    ...researcher see which of the multiplexed sgRNA was involved in a particular edit, and which type of edit was...open source and free for all non-profit uses. We invite you to try ICE today and let us know how it works...

  10. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    Published
    July 25, 2024, 1:15 p.m.
    ...in a competitive ELISA the reporter signal is inversely proportional to the level of antigen in the sample...in a competitive ELISA the signal intensity is inversely proportional to the amount of antigen in the sample...

  12. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ...gene commonly associated with CRISPR arrays and involved in spacer acquisition following infection, as ... we appreciate the opportunity to share these invaluable reagents with the scientific community. Note...

  13. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    Published
    Oct. 1, 2015, 2:30 p.m.
    ...in which your circuit of interest is putatively involved in regulating the behavioral output. For example...suggests that other regions of the brain may be involved in retrieval of remote contextual memories. This...

  14. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    Published
    Sept. 5, 2017, 1:58 p.m.
    ...DNA repair pathway. Accomplishing this usually involves simultaneously introducing a homologous DNA repair... (Paquet, Kwart, et al 2016). For those of you involved in the tedious business of iPSC clone picking,...

  15. March for Science

    Type
    Blog Post
    Published
    April 21, 2017, 2:30 p.m.
    ...march is meant to inspire scientists to be more involved in their communities (something already encouraged...mathematical constant “pi” supports scientists getting involved in politics. Disclaimer: The views presented ...

  16. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    Published
    March 27, 2018, 1:32 p.m.
    ...delivered through transfection of naked DNA. Other investigators who had been working to develop high efficiency..., which is particularly well suited for strand invasion, a key step in the homologous recombination pathway...

  17. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ...making them more useful for certain studies that involve surgery or imaging.  Rattus norvegicus. Image...entire developmental cycle. A majority of studies involving axolotls focus on embryonics and biochemistry....

  18. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    Published
    Nov. 16, 2021, 2:15 p.m.
    ...sample type. Sample Preparation This step can involve multiple smaller steps that all help to ensure ... Similar to Sample Preparation, this step can involve multiple smaller steps, but the aim is to preserve...

  19. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    Published
    June 27, 2024, 1:15 p.m.
    ...membrane-impermeant. With each cell division, the (invariant) amount of dye is reduced per cell (Figure 4a)...Harvard Medical School. His projects include investigating negative regulators of T cells and cytokine ...

  20. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    Published
    Sept. 14, 2016, 2:30 p.m.
    ... a suicide switch in cyanobacteria, synthetic inverters in E. coli, and biosensors in E. coli: Čelešnik...017129. Hsia, Justin, et al. "Modular Synthetic Inverters from Zinc Finger Proteins and Small RNAs." PloS...
Showing: 201 - 220 of 455 results