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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 406 results for: mag

Showing: 201 - 220 of 406 results

  1. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    Published
    Oct. 13, 2022, 1:45 p.m.
    ...knock in, base edit, activate, repress, and even image your favorite gene. With so many Cas9 reagents available...target strand based on the sequence of the pegRNA. Image from David Liu.   Activation and repression...

  2. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    Published
    Nov. 19, 2020, 2:15 p.m.
    ...-inducible Cre not only halted the neurological damage but completely reversed it, regardless of age or...corrected by CRISPR/Cas9 in wild-type human iPSCs. Image modified from Le et al., 2019. Gene editing ...

  3. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ...with mRNA and charged tRNAs to build a peptide. Image from Mariana Ruiz Villarreal. By the late 1980s... codons are under expressed compared to others. Image from Hiss et al., 2017. Most commonly, scientists...

  4. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ...groups have utilized dCas13 fusion proteins for imaging, tracking, modulating splicing, and regulating ...fluorescent fusion protein dLwaCas13a-NF for in vivo RNA imaging. In short, the Cas13a molecule brings a much anticipated...

  5. Tips for Getting a Faculty Position

    Type
    Blog Post
    Published
    July 20, 2023, 1:15 p.m.
    ...schools. I will emphatically deny that I have a magic formula to guarantee a reader the faculty job of...optimization of fluorescent proteins,  and live cell imaging approaches. Erik is also an avid long distance ...

  6. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    Published
    Aug. 23, 2016, 2:30 p.m.
    ...now to capture interesting key results, striking images, creative ideas, and engaging stories as you encounter...supportive audience? 7. Hone Your slides Use big images that are easy to see (and for heavens sakes, label...

  7. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...5, 6, 8, and 9 in two different brain regions.  Image modified from Aschauer et al., 2013, Figure 1. Used...both oligodendrocytes (Olig2) and neurons (NeuN). Image from Griffin et al., 2019, Figure 4. Used under ...

  8. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ... is twice the length of the monomer (~11.6 kb). Image generated by SnapGene software.  Thanks to ...multimer identification.   Fig. 2: Gel image of Plasmid 61564: pBAMD1-2 (~4.7 kb) digested with...

  9. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    Published
    March 8, 2021, 2:41 p.m.
    ...paper. Pretty much like an actual sandwich! S'mores image from Evan Amos.   After the transfer, some areas...chemiluminescence, is detected on x-ray film or with specific imaging devices. In most cases, the primary antibody, which...

  10. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ...aureus Cas9 (Sa-Cas) and deposited plasmid IF311: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-LSD1. LSD1 is a particularly.... These two vectors are part of the larger pMVP/pMAGIC cloning system, which contains Cas9 fusions for...

  11. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ... and obtain a knockout mouse in about 3 months. Image courtesy of Wenning Qin. Single-stranded DNA...electroporation is performed immediately after injection. Image from Ohtsuka et al., 2018. Used with permission ...

  12. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    Published
    July 12, 2016, 2:30 p.m.
    ...cellular repair mechanisms rush in to repair the damage. If all they can do is stitch the two broken ends...Mizutani E, Sato H, Kasai M, Ogawa A, Suchy F, Yamaguchi T, Nakauchi H (2018) Intra-embryo Gene Cassette...

  13. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ...cargo with the excision-only piggyBac™ transposase. Image courtesy of Transposagen.   How does piggyBac... selection cassette by excision-only piggyBac™. Image courtesy of Transposagen.   How does the piggyBac...

  14. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    Published
    June 8, 2021, 1:15 p.m.
    ...scientist was like. The word “scientist” conjured up images of men like Albert Einstein and Bill Nye in their...postdocs I’ve met have done things that I never even imagined before I set foot in the lab. They’re also more...

  16. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    Published
    March 6, 2018, 1:49 p.m.
    ...: PMC146043. 9. Nilsson, Stefan V., and Göran Magnusson. "Sealing of gaps in duplex DNA by T4 DNA ligase... using a capillary tube, probe DNA-immobilized magnetic beads and chemiluminescence detection." Analytical...

  18. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...dimers. (c) A double peak indicating 2 amplicons. Image credits (respectively): Zuzanna K. Filutowska; Selma...allows, you can fit in a quick game of Connect Four! Image created in BioRender.   Passive Reference Dyes...

  19. Beginner's Guide to Viral Vectors

    Type
    Blog Post
    Published
    June 2, 2017, 2:30 p.m.
    ... FAQ, and our viral service pages. Click on the Image Above to Download the Guide ...

  20. The Codon Guide to Synthetic Biology

    Type
    Blog Post
    Published
    Sept. 14, 2023, 1:15 p.m.
    ...prioritize open-access resources whenever possible.  Image credit: Niko McCarty; adapted from Bruce Wetzel ...
Showing: 201 - 220 of 406 results