Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 486 results for: mme

Showing: 201 - 220 of 486 results

  1. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ...than 50 cycles is recommended. Decrease your ramp rate. A rate of 2C/s is recommended to ensure an even...

  2. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    Published
    Sept. 6, 2018, 12:02 p.m.
    ...to purchase the Cas9 protein and a gRNA from a commercial vendor, this can often be expensive. An alternative...transcribed from ssDNA, which can be generated by commercial vendors such as IDT. These two components are...

  3. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    Published
    Sept. 8, 2016, 2:30 p.m.
    ...to this established dye structure have yielded commercial panels of advanced rhodamine fluorophores, such...Mensh, a wordsmith soldier of fortune, for helpful comments. Many thanks to our guest blogger, Luke D. Lavis...

  4. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    Published
    Oct. 6, 2015, 2:30 p.m.
    ...a time.   Not everything that is received is immediately transformed and stored. Some samples need to ...to organize samples within your lab, we highly recommend using barcodes! Barcoded tubes are easily accessible...

  5. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    Published
    May 15, 2014, 3:33 p.m.
    ... tag for protein purification and a number of commercial antibodies to GFP are available. Others: We’ve...interactions.   Do you have a favorite way to use GFP?  Comment and tell us how! And come back next week when we'll...

  7. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ... variable and rabbit constant domains and is recommended for use in western blots (Figure 1). If you prefer...worm-optimized wrmScarlet. mScarlet-I3, while initially dimmer, showed greater photostability and also matured...

  8. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    Published
    Oct. 19, 2017, 1:54 p.m.
    ...pair palindromic repeats separated by an 8 bp asymmetric core. Recombination between these sites can lead...discovered that it is not the exact sequence of the asymmetric target site core but its 8 bp length that is ...

  10. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    Published
    Dec. 13, 2016, 3:30 p.m.
    ...bevacizumab has recently been added to the treatment recommendations for non-resectable mesothelioma by the National.... Senate. If this bill passes, it will put an immediate stop to the use of asbestos, thus ensuring that...

  11. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...under CC-BY 4.0 License. Importantly, even commercial antibodies can be tethered on the viral surface...thick-section and whole-mount labeling techniques. The Trimmer lab’s anti-PSD-95 [K28/43R] recently passed their...

  12. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...Numpy, and Biopython.  We started at the usual recommended place: documentation. We oriented ourselves with...process. For example, our bbduk process created trimmed FASTQ files as output, which we specified as input...

  13. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ...unprecedented specificity: when purified, it could be programmed to generate specific cuts on the RNA target. ...non-dividing and primary cells, and can be easily programmed for new genes. In comparison with other large...

  14. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    Published
    Jan. 17, 2019, 1:38 p.m.
    ...librarians and data curators, IT managers, the commercialization office, the CIO’s office, and research computing...of the vibrant life sciences research and commercialization community in greater Boston, while at the ...

  15. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ... from Addgene now have full sequence data, we recommend directly analyzing gene sequences for codon optimization...thereby increase their abundance. For example, the commercial Rosetta E. coli strains express a variety of ...

  16. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ...we hear the word ‘antibiotic’, very often we immediately imagine a prescription for a bacterial infection...drug-resistant infections globally: final report and recommendations." London: Wellcome Trust & HM Government (2016...

  17. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...the study, or even review Facebook and Twitter comments. On We Share science, video usurps the written...the University of Maine, about her research on plummeting embryo survival rates. Video on We Share Science...

  18. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    Published
    Oct. 13, 2022, 1:45 p.m.
    ...rest of the genome.  2) The target is present immediately upstream of a Protospacer Adjacent Motif (PAM...where the edit is desired, and it performs the programmed edit within the editing window (bases 4-8 of ...

  19. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ...a single gene they want to activate, we would recommend using a cDNA overexpression vector rather than...closest to the transcription start site (TSS). We recommend targeting the guides to a region less than 200...

  20. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ... mycoplasma contamination. There are several commercially available mycoplasma detection kits that use... example, while freeze thaw is generally not recommended, Holic et al. found that lentivirus produced ...
Showing: 201 - 220 of 486 results