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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 507 results for: otos

Showing: 201 - 220 of 507 results

  1. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ...DNA sequence and has been suggested to cause cytotoxicity [5]. Second, instead of using a donor-plasmid...“Recombination between inverted loxP sites is cytotoxic for proliferating cells and provides a simple ...sequencing of retroviral insertion sites.,” Nat. Protoc., vol. 4, no. 5, pp. 789–98, Jan. 2009. PubMed ...

  2. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

    Type
    Blog Post
    Published
    Dec. 15, 2016, 3:57 p.m.
    ...Recombination-Based Method of Genetic Engineering. Nature protocols. 2009;4(2):206-223. doi:10.1038/nprot.2008.227...Bacteria Using Homologous Recombination. Current Protocols in Molecular Biology. 106:V:1.16:1.16.1–1.16.39...and Strains Check Out Other Molecular Biology Protocols ...

  3. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    Published
    March 6, 2018, 1:49 p.m.
    ...Resources on Addgene.org Basic Molecular Biology Protocols Protocol Videos Molecular Biology Reference Pages  ... critical reagents for many molecular biology protocols, including vector-insert joining for recombinant...

  4. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    Published
    Oct. 8, 2020, 1:15 p.m.
    ...from lowered pH due to microbial contamination. Photo by Kaustubh Kishor Jadhav. Mycoplasmas can remain...works in the tissue culture hood while wearing PPE. Photo by Kate Harten DeMaio. Cell lines and reagents...Addgene.org Check out Addgene's written and video protocols ...

  5. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    Published
    Oct. 9, 2018, 12:21 p.m.
    ...actually answer your question? If you're using a new protocol or reagents, do you understand all of the steps.... I've found cooking, gardening, hiking, and photography to be very satisfying. Finally, many PFs are...distance runner, gardener, cook, and outdoor photographer.  Additional Resources on the Addgene Blog ...

  6. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    Published
    Sept. 10, 2015, 2:30 p.m.
    ...longer available through Kopf). Additional general protocols on stereotaxic surgeries for viral injections ...in nontransgenic and cre driver mice. Current protocols in neuroscience / editorial board, Jacqueline ...using combinatorial viral approaches. Current protocols in neuroscience / editorial board, Jacqueline ...

  7. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...the highest frequency of genome editing events. Yamamoto Lab Multiplex CRISPR/Cas9 Assembly Kit: This ... efficiency >70%. CRMAGE is an incredibly fast protocol, with only 5 hours incubation time needed for ...single round of editing, and a subsequent curing protocol that requires only 2-3 hours incubation. Kondo...

  8. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    Published
    Sept. 28, 2020, noon
    ... efficiency >70%. CRMAGE is an incredibly fast protocol, with only 5 hours incubation time needed for ...single round of editing, and a subsequent curing protocol that requires only 2-3 hours incubation. E. coli...system has generally low efficiencies or can be proteotoxic when used in Mycobacterial species, Sarah Fortune...

  9. #DeckTheLab to Win a Tasty Winter Treat This Holiday Season

    Type
    Blog Post
    Published
    Nov. 30, 2015, 3:30 p.m.
    ...festivities! Help us spread some cheer by snapping a photo of you and your labmates celebrating the holidays...Festivus— you name it, we want to see it.      All photos tagged with #DeckTheLab throughout the month of...

  10. Addgene's 20th Anniversary Party

    Type
    Blog Post
    Published
    June 11, 2024, 1:15 p.m.
    ...party, we shared memories of Addgene's past. Framed photos and slideshows shared some of the moments we captured...over the years, including an album of Addgenie pet photos. The famous Addgene jacket, home to a questionable...

  12. Top Requested Plasmid of 2017 - pMD2.G

    Type
    Blog Post
    Published
    Jan. 10, 2018, 4:13 p.m.
    ... preps. You can find our lentivirus production protocol on our website and tips for titering your lentivirus...check out our lentivirus guide page. For more protocols and information, be sure to check out the Trono...

  14. PEI Calculator for Planning AAV Packaging Transfections

    Type
    Blog Post
    Published
    Jan. 23, 2018, 2:04 p.m.
    ...Vectors for In Vitro and In Vivo Use." Current Protocols in Molecular Biology (2007): 16-25. PubMed PMID...to Making AAV? Check out Addgene’s Step-By-Step Protocol Pressed For Time? Order Ready-to-Use AAV from ...

  16. New Viral Vectors - March 2024

    Type
    Blog Post
    Published
    March 5, 2024, 2:15 p.m.
    ...contains a biosensor that detects both serotonin and serotonergic drugs. Check out these new vectors, and...

  17. More Data for You: Find Articles Citing Addgene Plasmids

    Type
    Blog Post
    Published
    June 16, 2015, 2:15 p.m.
    ...interest, which may help you identify relevant protocols and experimental data Find other related plasmids...Sequencing Results ICYMI: Addgene’s Plasmids 101 eBook Protocols and Plasmid References @Addgene  ...

  19. Reflections and Looking Ahead

    Type
    Blog Post
    Published
    Feb. 25, 2025, 2:15 p.m.
    ...Gonzalez Bolivar, S., Alende, C., Ruiz Moleon, V., Fotouhi, M., Alqazzaz, M., Southern, K., Alshafie, W., ...platform for antibody characterization. Nature Protocols. https://doi.org/10.1038/s41596-024-01095-8 Kahn...

  20. Inside Addgene's 10 Year Anniversary Gala

    Type
    Blog Post
    Published
    July 3, 2014, 3:48 p.m.
    ...towards the interactive photo graffiti wall. I had a lot of fun playing the prototype of an interactive game...
Showing: 201 - 220 of 507 results