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...watch my webinar, “Not Networking 101-Building Relationships for Success”. Choose 1-2 tactics to try. One...be a bit random because you never know which relationships will take off, or who your new contact will ...current organization but also important to build relationships outside your organization. The mentor in your... doing it again next month?” Some mentoring relationships grow naturally out of friendships or work interactions...other ways they can help you grow. Mentoring relationships can last for an hour or for a year. Some click...

...is the question. When presenting on building relationships (also known as “networking”), one of the most...networking to start, build, and track professional relationships. Here are my seven best tips beyond the basics...on LinkedIn!” resulting in just a little more relationship. Pro tip: Once you are connected with someone...connection requests LinkedIn is a place to build relationships. If you don’t add a personal note, you waste...Do not post or comment on anything that is not relevant to business or volunteer organizational work. ...

...some researchers have suggested they may have relevance to human health (Fogarty et al., 2024), and others...created as whole-genome sequencing becomes more relevant (Garcillán-Barcia et al., 2023). In this post,...organization doesn’t necessarily reflect genetic relationships or plasmid incompatibility. Plasmids may also...than one function: for example, Gateway cloning relies on genes found on an E. coli fertility plasmid ...cloning. In addition, scientists have engineered entirely new types of plasmids without clear analogues ...

... the ultimate resolution can involve ideas not related to the actual data collection task but still be...resolution. Creative negotiation can also decrease or entirely eliminate the feeling of conflict many of us associate...negotiation skills will help you strengthen relationships with your colleagues and supervisors and lead...negotiation skills are key to maintaining and growing relationships with your colleagues within and outside of your... what you want and can also strengthen your relationships with others who know that working with you will...

...emerging technologies to remain competitive and relevant. Enter: the journal club.  A journal club is ... in the project.  Once we gauged interest from relevant parties, and the company at large, by polling ...we as scientists often prefer data. At Addgene we rely on polls and surveys. A mix of closed (multiple ...should be continually evaluated and assessed for relevance.  Concluding thoughts Journal clubs are a great...they should be regularly assessed for continued relevance and if needed, rebooted or disbanded all together...

...demonstrated that SHERLOCK could reliably distinguish between Zika and a closely-related virus, Dengue from multiple...simple, portable, and inexpensive platforms to reliably detect nucleic acids at the atomolar level. The... easy to transport and work rapidly, providing reliable results in as little as an hour. Find the SHERLOCK...from cell-free DNA fragments as well as health-related single nucleotide polymorphisms (SNPs) from human...

...stand out with key words and by putting your most relevant experience at the top. You may also consider visiting...opening, you can send your contact an email with the relevant info and they just might personally hand your ...as your own adviser will probably have a lot of relevant experience and information to offer. However, ...Importantly, find a good mentor that can share relevant experience and resources with you to ease the ...the way. Make connections, and nurture those relationships. Then when you are ready to apply for your dream...

...only ever a small fraction of all data directly relevant to a particular experiment, series of experiments...should have integrations with a broad range of relevant generic and science-specific tools. In addition... added on a regular basis, so the ecosystem of related tools is always growing. Beyond the ability to ...understand and/or attempt to reproduce research relating to data deposited into a repository access to ...can be viewed – and conceivably additional work relating to reproducing the original research can be documented...

...a different method of selection entirely. Auxotrophic selection relies on the host organism being unable...basis of shuttle vectors, which can function in unrelated host species, even from completely different kingdoms...replication machinery. A shuttle vector designed for unrelated bacteria should include oris for both target species... lab tools, and they include both vectors for unrelated bacterial species and those that can shuttle between...

...multiplexing is that you can ask how multiple proteins relate to one another in the same sample. For this strategy...BioRender.com   The first couple of steps are preliminary steps that are not exclusive to IF, but they ... The biggest difference between IF and ICC/IHC relates to the type of secondary antibody you use, so as...antibody that you consider in order to determine how reliable the antibody seems and what sort of protocol you..., will help make antibodies more effective and reliable in the future.  We hope that this post has shed...

...methods roughly fall into two categories: those that rely on exogenous enzymes and those that use endogenous...). Both of these systems are powerful, but they rely on exogenous enzymes, require multiple transformation...The most commonly used counter-selection system relies on the sacB gene product of B. subtilis (Hmelo ...would allow stronger counter-selection than one merely co-opted toward that aim (i.e. sacB). Importantly...arabinose promoter worked too. Rhamnose induction relies on the transcriptional activator rhaS. To allow...

...particle orientation distribution and Fourier-shell correlation curve (D), and the reconstructed 3D map with ...times that size (demonstrated up to 10.5 kb) by relying on a site-specific serine recombinase. First, the...on opposite DNA strands. When the prime editor releases the DNA, the two 3’ flaps containing the edited...editor, Coupled Histone tail for Autoinhibition Release of Methyltransferase (CHARM), with several advantages...the methyltransferase binds to H3K4me0 on D3L, releasing the autoinhibition and stimulating the methylation...

...looser gate here. I am not worried about including irrelevant events in my analysis, as these will get excluded...from your single cells, as duplicates can’t be reliably analyzed on their staining signals. For this, ...    Figure 4: (A) Using the proportional relationship between FSC-H and FSC-A we can gate on single...displaying your events. You are free to choose any size-related parameter, such as FSC-H, FSC-A, SSC-H, SSC-A, ...Figure 6: (A) A flow plot showing two stains in relation to each other. Using a quadrant gate, I can divide...

...45), there are also initiatives to create more reliable reagents for future research. I’ve talked to many...many researchers who have complaints about the unreliability of antibodies. IPI is working to address this...repeat previously published results. This over-reliance on the perfect story for a “high impact” publication...ways to publicise negative data - we don’t have to rely on traditional journals. There’s social media - ...publishing community views reproducibility, how industry relies on reproducibility, and how you can push for more...

... Porrot, F., Bohl, D., Marmé, F., Chérel, Y., Salvetti, A., Hurtrel, B., Heard, J.-M., Rivière, Y., & ...your specific experimental system, rather than relying on other systems as proxies.  Finally, the size...consider. First, the titer required is inversely related to transduction efficacy: the higher the efficiency...against the Tetracycline-Dependent Transactivator Correlates with Long-Term Doxycycline-Regulated Transgene...

...assemble a rough guide to the science of Ebola and related resources. Ebola Basics A few basics: Ebolavirus...outbreak and earlier ones, along with links to the relevant scientific literature.  -In light of the current...Medicine have made their complete collection of relevant papers freely available here. -PLoS and The New...of Medicine have similarly curated their Ebola-related content. -And the latest updates on the ongoing...

...attracting and keeping diverse talent in science related careers. Failure to strengthen the talent pipeline...project and the findings were captured in this just released report “Enhancing Dissemination of Evidence-Based... join us for the pd|hub Workshop Report Public Release Event scheduled for March 4th from 2:30-4:00 pm...science career resources Browse all blog posts related to science careers Download the Science Career ...

.... During this time, scientists discovered many related immune mechanisms that could target foreign DNA... effectively. The current classification system relies on a combination of sequence similarity, phylogenetic...same name but be completely different! They are merely linked by a similar function. After analysis, CRISPR...various functions. Class 2 Class 2 CRISPR systems rely on a single protein Cas effector and are likely ...broken down into type II, type V, and type VI, each relying on a unique Cas effector — Cas9, Cas12, or Cas13...

...called a Glycosylase Base Editor, or GBE, for its reliance on the glycosylase UNG to crease abasic sites ...hypothesized that the CGBE edit process occurs entirely while Cas9 is bound, keeping the two DNA strands...regions that were already accessible. It's not entirely clear why this is, but it might point to a more...: CGBEs and indels Cytosine transversion editors rely on a multi-step cellular repair pathway that is ... GBE Glycosylase Base Editor A base editor that relies on DNA glycosylation to create an abasic site. ...

...often happen when we allow ourselves moments of release and mental freedom. Practical approaches Engaging...to detach from the constant stream of thoughts related to lab research and gain fresh perspectives. Such...encourages exploration of tangents or seemingly unrelated ideas. It's beneficial to carry a notebook at ... identify areas in need of improvement. These unrelated ideas might hold the key to unlocking new perspectives...