We narrowed to 262 results for: rigi

1. Capturing a Moment with Marc Zimmer’s “The State of Science”

   Type

   Blog Post

   Published

   July 30, 2020, 1:15 p.m.

   ...story has its own Franklin in Doug Prasher, who originally isolated the GFP gene but was not included amongst...

2. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

   Type

   Blog Post

   Published

   Aug. 9, 2016, 2:30 p.m.

   ...Aneesh Karve, CTO at Quilt Data. This post was originally published on the Quilt Genomics Blog and is republished...

3. Lentiviral Vector Uses and Overview

   Type

   Blog Post

   Published

   May 19, 2016, 2:30 p.m.

   ...Although the 2nd generation system is safer than the original system, the possibility of creating a replication-competent...

4. CRISPR Antimicrobials

   Type

   Blog Post

   Published

   May 3, 2016, 2:30 p.m.

   ...systems. Phagemids are plasmids containing a phage origin of replication that can be packaged into replication-incompetent...

5. Tips for Screening with Yeast Two Hybrid Systems

   Type

   Blog Post

   Published

   Oct. 22, 2015, 2:30 p.m.

   ...proteins of interest has occurred. Although the original Y2H systems utilized the yeast Gal4 activator,...

6. Plasmids 101: Protein tags

   Type

   Blog Post

   Published

   Dec. 11, 2014, 4:26 p.m.

   ...combinations still include at least one element from the original TAP tag. The terms dual-labeling and dual-tagging...

7. Negotiating Work and Life: How to Find the Joy

   Type

   Blog Post

   Published

   March 20, 2014, 3:04 p.m.

   ...This article was originally published on the Society for Laboratory Automation and Screening (SLAS) Electronic...

8. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

   Type

   Blog Post

   Published

   July 29, 2025, 1:15 p.m.

   ...Additionally, the TAM changes based on the species of origin of the TnpB protein, much like how PAM sites change...

9. QC Sequencing Technologies at Addgene

   Type

   Blog Post

   Published

   July 15, 2025, 1:15 p.m.

   ...relies on sequencing by synthesis, but modifies the original Sanger procedure to improve throughput by increasing...

10. Plasmids 101: Shuttle Vectors

    Type

    Blog Post

    Published

    May 20, 2025, 1:15 p.m.

    ...population. A bacterial chromosome has a single origin of replication (ori), where DNA replication begins...

11. CRISPR 101: Any Base Transversion Editors

    Type

    Blog Post

    Published

    April 1, 2025, 1:15 p.m.

    ... (AAG). When adenine base editors (ABEs) were originally being developed for A → G edits, researchers ...

12. Twenty Years of Sharing: Expanding Accessibility

    Type

    Blog Post

    Published

    Nov. 21, 2024, 10:47 p.m.

    ...Bacterial Expression Systems AAV Plasmids Blogs Origin of Replication What is a Plasmid? How to Design...

13. Antibodies 101: The Four ELISAs and When to Use Them

    Type

    Blog Post

    Published

    July 25, 2024, 1:15 p.m.

    ...target antigen in a sample. While Engvall et al. originally developed the ELISA assay to measure antibody...

14. Tips for Writing a Good Cover Letter

    Type

    Blog Post

    Published

    May 21, 2024, 1:30 p.m.

    ... you're ready to go! Good luck! This post was originally written by Maria Soriano-Carot in September 2016...

15. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type

    Blog Post

    Published

    Jan. 24, 2023, 2:15 p.m.

    ...arabinose-inducible promoter pBAD and a low-copy number origin of replication (p15A) worked best under a wide ...

16. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type

    Blog Post

    Published

    Aug. 13, 2019, 12:58 p.m.

    ...ideal system to observe embryogenesis live. The origins, divisions, and movements of each of the thousands...

17. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type

    Blog Post

    Published

    Sept. 16, 2020, 1 p.m.

    ... Originally published Jul 14, 2015 and last updated Sep 16, 2020 by Beth Kenkel.  CRISPR genome editing...

18. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type

    Blog Post

    Published

    Aug. 20, 2020, 2:30 p.m.

    ...Originally published Nov 12, 2015 and last updated Aug 20, 2020. Cas9 can be used to modify any desired...

19. New CRISPR Tools: Cas7-11 and PASTE

    Type

    Blog Post

    Published

    Feb. 17, 2022, 2 p.m.

    ...strongly supported their initial findings.  The team originally suspected that this new Cas7-11 might be phylogenetically...

20. Finding nucleic acids with SHERLOCK and DETECTR

    Type

    Blog Post

    Published

    April 16, 2020, 1 p.m.

    ...Originally published Aug 30, 2018 and updated April 16, 2020. Sensitive and specific nucleic acid detection...