We narrowed to 512 results for: sam
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TypeBlog PostPublishedJan. 12, 2021, 2:15 p.m.... Attend events for one organization or go to the same conferences/meetings over and over to get to know...volunteer). Naturally running into or working with the same people is often the easiest way to build relationships...
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Summer SciComm Series: A PhD in Science Communication
TypeBlog PostPublishedAug. 23, 2022, 1:15 p.m....fitting that I’ve ended up (again) dispelling the same myths and misconceptions fifteen years later. What..., peer-reviewed study. The second group read the same abstract but with the names reversed, meaning that... -
22 Hot Plasmid Technologies from 2014
TypeBlog PostPublishedJan. 6, 2015, 5:21 p.m....use of the two systems in different tissues of the same animal. Flipbow additionally incorporates SUMO tags...which express self-excising Cre recombinase from the same transgene as the Brainbow cassette, simplifying ...expression plasmids for a given species are based on the same backbone to permit expression levels to be directly... -
Addgene’s Viral Service - Why Virus? Why Now?
TypeBlog PostPublishedMarch 23, 2017, 2:30 p.m....2004. We’ve already distributed over 500 viral samples to scientists all over the world. Now that the ..., tracked production and distribution of viral samples. The Office Team made sure we could ship internationally... -
Why and how to start studying chromatin (with Addgene's help)
TypeBlog PostPublishedJune 12, 2018, 11:40 a.m....Single Nucleus from a Set of Mitotic Chromosomes. Samwer M, Schneider MWG, Hoefler R, Schmalhorst PS, Jude... -
Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies
TypeBlog PostPublishedJuly 28, 2017, 1:30 p.m....recombination so that the transgenes can be inherited on the same chromosome. Targeted Insertion allows for direct...variants of YFG because the transgenes are found in the same locus across fly lines (i.e. there won’t be positioning... -
Plasmids 101: Screens vs. Selections
TypeBlog PostPublishedNov. 10, 2022, 2:15 p.m....defined disease phenotype. These screens employ the same downstream manipulations as a forward or reverse...unlike in screens. Selections begin and end with the same steps as screens – they start with manipulations... -
Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)
TypeBlog PostPublishedSept. 26, 2024, 1:15 p.m....allows both insert and vector to be digested in the same tube. Alternatively, SLIC can also work directly...using multiple fragments that share homology in the same reaction. In these cases, the use of another cloning... -
Clear and Accessible Writing
TypeBlog PostPublishedFeb. 15, 2024, 2:15 p.m....that helps visually impaired readers receive the same information as sighted readers. Figure...Excellent! We asked our workshop participants to do the same thing, and even though only about 10% of them had... -
Viral Vectors 101: Inverted Terminal Repeats
TypeBlog PostPublishedOct. 19, 2023, 1:15 p.m....cis-acting elements, which means that they are in the same DNA molecule as the gene that they package. In a.... M., Dobbins, A. L., Li, C., Hirsch, M. L., & Samulski, R. J. (2020). Adeno-Associated Virus Serotype-Specific... -
Sweating the Small Stuff: Details in the Lab
TypeBlog PostPublishedFeb. 8, 2022, 2:15 p.m....running it unchanged over and over again, and the same goes for your workflow. Next time you make a mistake... to match your plate layout can help you track samples as you add them. Moving your reagents from your... -
Antibodies 101: Buffers, Storage, and Conjugates
TypeBlog PostPublishedFeb. 1, 2022, 2:15 p.m.... the antibodies time-sensitive, using precious samples, or being compared to other data in a manner that...signaling molecule, that all need to be detected in the same assay. This is one of the most complex subjects ... -
REPLACR Mutagenesis: Replacing In Vitro Recombination Methods
TypeBlog PostPublishedFeb. 10, 2016, 3:30 p.m....insertions, deletions, and substitutions - at the same efficiency as Gibson Assembly and GeneArt cloning...plasmid. For insertions, it’s possible to use the same technique just using a large primer known as a megaprimer... -
Form Your Own Peer Mentoring Group: A How-To Guide for Scientists
TypeBlog PostPublishedFeb. 18, 2014, 4:22 p.m....Allows all group members to lead and learn at the same time Broadens the network of the participants Can...managing to get together. If you all work at the same place, a 1-2 hour lunch on a regular schedule might... -
Kiran Musunuru on the Newest TALEN Genome-Editing System
TypeBlog PostPublishedDec. 10, 2013, 3:13 p.m....of individuals and find several mutations in the same gene or in multiple genes, all of which seem to ...insert all of those variants into stem cells with the same genetic background and do an apples-to-apples comparison... -
Choosing the B(right)est Fluorescent Protein: Photostability
TypeBlog PostPublishedJune 8, 2017, 2:30 p.m....This can differ between fluorescent proteins in the same spectral class (Cranfill et al, 2016). This non-...comparison of several fluorescent proteins of the same spectral class on the microscopy system and in the... -
Plasmids 101: CcdB - The Toxic Key to Efficient Cloning
TypeBlog PostPublishedDec. 8, 2016, 3:30 p.m.... the ccdB aspect, Gateway takes advantage of the same principle that cells will not propagate while expressing.... While these two strains ultimately perform the same function, there is some evidence that ccdB Survival... -
RaPID Detection of RNA-protein Interactions
TypeBlog PostPublishedAug. 16, 2018, 12:40 p.m....non-physiological RNA-protein interactions due to sample processing. Require prior knowledge that the protein...control). BioID2 had no detectable signal at the same time point. E. coli’s BirA* was tested at its optimum... -
Lighting Up Cell Signaling with Photoswitchable Kinases
TypeBlog PostPublishedJuly 25, 2017, 1:20 p.m....hypodermis and gut cells of C. elegans causes the same phenotype. Activation of the Raf-MEK-ERK signaling...signaling pathway with psRaf1 or psMEK1 in these same cells by 24-48 hours exposure to blue light also induced... -
CRISPR 101: Cas9 vs. The Other Cas(s)
TypeBlog PostPublishedDec. 8, 2022, 2:15 p.m....isolated which target and cleave RNA through the same mechanism as traditional spCas9. If you are looking...Cas13 and has been proposed to be useful for the same applications (RNA knockdown, RNA editing, etc.)....