We narrowed to 512 results for: sam

...fragments containing the desired mutations. This same process can be used simultaneously for the generation...cassette, ready for transformation into yeast that same day.  The field of synthetic biology has led to ...

...fitting that I’ve ended up (again) dispelling the same myths and misconceptions fifteen years later. What..., peer-reviewed study. The second group read the same abstract but with the names reversed, meaning that...

... Attend events for one organization or go to the same conferences/meetings over and over to get to know...volunteer). Naturally running into or working with the same people is often the easiest way to build relationships...

...use of the two systems in different tissues of the same animal. Flipbow additionally incorporates SUMO tags...which express self-excising Cre recombinase from the same transgene as the Brainbow cassette, simplifying ...expression plasmids for a given species are based on the same backbone to permit expression levels to be directly...

...allows both insert and vector to be digested in the same tube. Alternatively, SLIC can also work directly...using multiple fragments that share homology in the same reaction. In these cases, the use of another cloning...

...Single Nucleus from a Set of Mitotic Chromosomes. Samwer M, Schneider MWG, Hoefler R, Schmalhorst PS, Jude...

...recombination so that the transgenes can be inherited on the same chromosome. Targeted Insertion allows for direct...variants of YFG because the transgenes are found in the same locus across fly lines (i.e. there won’t be positioning...

...2004. We’ve already distributed over 500 viral samples to scientists all over the world. Now that the ..., tracked production and distribution of viral samples. The Office Team made sure we could ship internationally...

...cis-acting elements, which means that they are in the same DNA molecule as the gene that they package. In a.... M., Dobbins, A. L., Li, C., Hirsch, M. L., & Samulski, R. J. (2020). Adeno-Associated Virus Serotype-Specific...

...running it unchanged over and over again, and the same goes for your workflow. Next time you make a mistake... to match your plate layout can help you track samples as you add them. Moving your reagents from your...

... the antibodies time-sensitive, using precious samples, or being compared to other data in a manner that...signaling molecule, that all need to be detected in the same assay. This is one of the most complex subjects ...

...defined disease phenotype. These screens employ the same downstream manipulations as a forward or reverse...unlike in screens. Selections begin and end with the same steps as screens – they start with manipulations...

...that helps visually impaired readers receive the same information as sighted readers.    Figure...Excellent! We asked our workshop participants to do the same thing, and even though only about 10% of them had...

...insertions, deletions, and substitutions - at the same efficiency as Gibson Assembly and GeneArt cloning...plasmid. For insertions, it’s possible to use the same technique just using a large primer known as a megaprimer...

...Allows all group members to lead and learn at the same time Broadens the network of the participants Can...managing to get together. If you all work at the same place, a 1-2 hour lunch on a regular schedule might...

...of individuals and find several mutations in the same gene or in multiple genes, all of which seem to ...insert all of those variants into stem cells with the same genetic background and do an apples-to-apples comparison...

...Because we have a resource like Addgene to all get the same virus.” — Grad student visiting Addgene’s booth ...quality reagents, and we realized we could use the same principles to provide recombinant antibodies, another...

...non-physiological RNA-protein interactions due to sample processing. Require prior knowledge that the protein...control). BioID2 had no detectable signal at the same time point. E. coli’s BirA* was tested at its optimum...

...hypodermis and gut cells of C. elegans causes the same phenotype. Activation of the Raf-MEK-ERK signaling...signaling pathway with psRaf1 or psMEK1 in these same cells by 24-48 hours exposure to blue light also induced...

...This can differ between fluorescent proteins in the same spectral class (Cranfill et al, 2016). This non-...comparison of several fluorescent proteins of the same spectral class on the microscopy system and in the...