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...strategy for increasing protein production. If you are trying to express a protein in its native host there is...

...organisms, they can be problematic for researchers trying to express proteins in heterologous hosts. If you...

...and free for all non-profit uses. We invite you to try ICE today and let us know how it works for you! Read...the sample name or on its corresponding bar graph entry. Clicking to initiate the in-depth analysis will...

...organizations like Science from Scientists, which try to bring real scientists into schools to provide ... job” could entail any number of career paths--industry, nonprofit organization, law office, journal editor...

...opportunity for your classroom, I highly recommend you try implementing one. It's likely only a matter of time... in their careers? A CURE for Biochemistry Undergraduate biochemistry courses are often associated with...Minnesota State (and now Professor of Chemistry and Biochemistry at the University of San Diego), decided...Sharpless was awarded this year’s Nobel Prize in Chemistry, he joined an elite club, becoming just the fifth...presented their findings at the American Society for Biochemistry and Molecular Biology Annual Meeting. Some of...inquiry and research-based learning in a year-long biochemistry laboratory: Part I – guided inquiry – purification...dehydrogenase, and green fluorescent protein. Biochemistry and Molecular Biology Education. 38(5):317-323...

...the scales in your favor for an HDR edit, you can try one or more of the below suggestions. Disable the...

...issues related to AAV vectors, many from people trying to answer the exact same question I was: What AAV...

...applying for technical support positions, you should try to match your expertise with that of the company....are often found in sales or lab-based roles in industry. What to look forward to in a technical support...offer an easy transition from an academic lab to industry without being too commercial or “sales-y”. The...be a good jumping off point for other roles in industry. Many thanks to Eric Perkins and Stephanie Hays...

... these parameters, here are a few things you can try: Increase the number of cycles. After additional...

...societal standpoint, we can all work together to try and prevent outbreaks of drug resistant bacteria ...

... against each other at the same time in order to try and access the entire interactome of a set of proteins...

...their protein of interest. After several decades of trying to address these challenges, researchers have amassed... used for western blots, IP, co-IP, IF, flow -cytometry; can occassionally interfere with protein folding... used for western blots, IP, co-IP, IF, flow -cytometry, but rarely used for purification as elution requires...

...not always a straightforward process. Rather than try to directly detect your gene of interest, scientists...advantage of an element called an Internal Ribosome Entry Site (IRES) to allow for initiation of translation...encephalomyocarditis virus RNA directs internal entry of ribosomes during in vitro translation. Jang et...

...implicated in human diseases. His spare time is spent trying to raise the alpha isoform of GSK-3 to the same...

...exhaustive, but should be a good place to start when trying to pick your perfect promoter! Eukaryotic Promoters...E. coli tryptophan operon Repressible Gets turned off with high levels of cellular tryptophan. lac General...

...position and directionality of the sequence you are trying to clone. Figure 2: MoClo assemblies depend...

...Decades later and after years of painstaking work trying to isolate the causative gene for Rett Syndrome...

...hard to conceptualize where the populations are, try drawing a quadrant over the plot that divides it ...data in the literature. Data reported from flow cytometry experiments can be a little challenging to understand... on how to read a flow plot.  Dot plots Flow cytometry is a method that allows users to analyze single...Created with biorender.com.   The goal of flow cytometry is to use increasingly more stringent markers ...in the same order. However, in an actual flow cytometry run, all markers are read in parallel.  Let’s ...more things to learn about when diving into flow cytometry data. There’s different ways to use FSC and SSC...resources on the Addgene blog Conventional vs. Flow Cytometry Plasmid-based Recombinant Monoclonal Antibodies...

... new to the mARG expression are advised to first try to produce GVs in their own lab using the most reliable...is easy: simply grow cells in Lennox LB (10 g/L tryptone, 5 g/L yeast extract, and 5 g/L NaCl) or on LB-agar...Magnetic Imaging and Control of Cellular Function. Biochemistry 56, 5202–5209 (2017). Bourdeau, R. W. et al....

...this and are still struggling with idea generation, try this thought exercise: Consider the issue as if there...