We narrowed to 261 results for: uros

1. Multiplexed Overexpression of Regulatory Factors (MORF) collection

   Type

   Blog Post

   Published

   March 30, 2023, 1:15 p.m.

   ...predicted cell type (e.g., axon-like projections for neuron predicted differentiation), and 15/17 morphologically...

2. Visualizing Translation at the Single Molecule Level

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   Blog Post

   Published

   Aug. 1, 2017, 1:15 p.m.

   ...Using translation inhibitors cycloheximide and puromycin, Halstead et al. verified that translation is ...

3. Hot Plasmids - January 2023

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   Blog Post

   Published

   Jan. 31, 2023, 2:15 p.m.

   ...length GFP and converted to recombinant form by the NeuroMab Facility Isotype IgG2a Differences:  Distinct...

4. R Bodies: Membrane-Rupturing Microscopic Tools

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   Blog Post

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   April 14, 2016, 2:30 p.m.

   ...R-body producing recombinant E. coli on paramecia. European Journal of Protistology 48, 290–296 (2012). PubMed...

5. Synthetic Photobiology: Optogenetics for E. coli

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   Blog Post

   Published

   Sept. 8, 2015, 2:30 p.m.

   ... forget that this technique isn’t limited to neuroscience. In fact, precise light-based control of biological...

6. Plasmids 101: Mammalian Vectors

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   Blog Post

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   March 25, 2014, 3:15 p.m.

   ...80S; inhibits chain elongation. 50-500 ug/mL Puromycin pac HeLa, 293HEK, Jurkat T cells Inhibits protein...

7. AAV Q&A with Tim Miles

   Type

   Blog Post

   Published

   May 17, 2022, 1:15 p.m.

   ...microglia? I have been using the AAV.PHP.eb for neuron, oligodendrocyte, and astrocyte specificity and...

8. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

   Type

   Blog Post

   Published

   March 17, 2020, 1:32 p.m.

   ...Richter MF, Zhao KT, Eton E, Lapinaite A, Newby GA, Thuronyi BW, Wilson C, Koblan LW, Zeng J, Bauer DE, Doudna...

9. Developing a cold tolerant E. coli using specialized chaperones

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   Blog Post

   Published

   Jan. 28, 2020, 2:35 p.m.

   ... a decidedly uncomfortable 45°C). Their more adventurous cousins are the extremophiles - the legends who...

10. Clear and Accessible Writing

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    Blog Post

    Published

    Feb. 15, 2024, 2:15 p.m.

    ...guidelines.  Which is why, at the Society for Neuroscience conference in November 2023, we hosted our very...

11. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

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    Blog Post

    Published

    May 3, 2018, 1:48 p.m.

    ... AAV-mediated delivery to iPS-derived cortical neurons, dCasRx-mediated exon skipping improved the ratio...

12. Dos & Don'ts When Publishing a Scientific Manuscript

    Type

    Blog Post

    Published

    May 22, 2014, 4:02 p.m.

    ...journals, has joined a pilot project from the Neuroscience Information Framework (NIF) called the Resource...

13. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type

    Blog Post

    Published

    Dec. 10, 2013, 3:13 p.m.

    ...disease in question – cardiomyocytes, adipocytes or neurons, for instance – to see how those mutations influence...

14. What's New in CRISPR - November 2021

    Type

    Blog Post

    Published

    Nov. 18, 2021, 2:15 p.m.

    ...Cas12f from a different species, Acidibacillus sulfuroxidans, AsCas12f1. They found that this system, without...

15. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

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    Blog Post

    Published

    March 3, 2020, 2:15 p.m.

    ...many FRET-opsin GEVIs. With an increase in the neuron’s membrane potential, Ace absorbance increases and...

16. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

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    Blog Post

    Published

    Jan. 31, 2019, 1:32 p.m.

    ...multiple cell types: fibroblasts, myofibroblasts, neuroendocrine, adipose, immune, inflammatory, angiogenic ...

17. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

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    Blog Post

    Published

    July 12, 2021, 1:15 p.m.

    ...vectors (for lentiviral packaging) and contain an IRES-Puro marker for stable cell clone selection. For more...

18. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type

    Blog Post

    Published

    Oct. 6, 2020, 1:15 p.m.

    ...using CRISPR–dCas9-activator transgenic mice. Nat Neurosci 21:440–446 . https://doi.org/10.1038/s41593-017...

19. Fluorescent Proteins 101: Introduction to FRET

    Type

    Blog Post

    Published

    June 27, 2017, 1:03 p.m.

    ...molecular “spring”) (Meng et al., 2008), and monitor neuronal activity (voltage sensors), to name a few.   Find...

20. Adapting Toehold Switches to Detect Zika Virus

    Type

    Blog Post

    Published

    Aug. 30, 2016, 2:30 p.m.

    ...important in the current outbreak because of the neurological symptoms associated with the American-lineage...