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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 576 results for: BASE;

Showing: 441 - 480 of 576 results

  7. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ...Blast Most of Addgene’s lentiviral inventory is based on CRISPR tools, though we do provide a few non-...

  8. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    Published
    Jan. 14, 2016, 3:30 p.m.
    ...reliable sequence data back for the first 50-100 base pairs of your sequencing read. You can expect to...mammalian selection markers. You don’t need a fancy database to organize your own plasmid collection, although...help of DNA-editing software which integrates our database of over 2000 primers. Two 96-well PCR plates are...Finally, you will want to have a well-organized database of expected sequences, either for the inserts ...Get Plasmid Backbone Information from Our Vector Database  ...

  11. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ... CRISPR guides that flank regions of hundreds of base pairs or more can simultaneously introduce a pair...

  12. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    Published
    Feb. 25, 2014, 7:11 p.m.
    ...marker help synthesize? Counterselection? (growth-based positive selection for the loss of the marker gene...

  13. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...provide recombinant antibodies, another plasmid-based product that, with Addgene’s support, could fill...

  15. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ...beyond generating frameshift mutations or single base pair edits, these enzymes are worth taking a look...Cas3 DNA digestion can proceed up to multiple kilobases, but only in one direction. Cas3 is thus not helpful...

  17. RNA Extraction Without A Kit

    Type
    Blog Post
    Published
    March 24, 2020, 1:15 p.m.
    ...and RNAProtect® from Qiagen, are ammonium sulfate-based solutions that work by inhibiting RNase activity...

  19. Human Germline Editing Using CRISPR

    Type
    Blog Post
    Published
    Aug. 10, 2017, 2:19 p.m.
    ... at present.   Another issue surrounding CRISPR-based therapy is the level of testing needed to define...

  20. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    Published
    April 10, 2018, 12:56 p.m.
    ...difficult for individual labs to complete. The nanobody based RANbody platform from the Sanes Lab overcomes this...

  28. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type
    Blog Post
    Published
    March 12, 2020, 1:15 p.m.
    ...used to restrict expression to a single cell type based on the overlap in cell types both promoters expressed...Institute of Molecular and Clinical Ophthalmology Basel recently took a different approach to enhance specificity...targeting the same cell class in humans (R=0.67).  A database of the microscopy images of all AAVs that reproducibly...

  30. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    Published
    July 18, 2019, 12:55 p.m.
    ...The Sternberg lab in 2016 created cGAL, a GAL-4 based bipartite expression system that can regulate gene... is curated and added to other databases including wormbase and flybase. microPublication is run and maintained...pages and curated databases for scientists including Wormbook, Wormatlas and Wormbase. And scientists have...institute developed a 3’UTR database specifically for C. elegans. This database provides detailed information...coding mRNAs, and annotations extracted from other databases (Mangone et al., 2008).  OpenWorm OpenWorm is ...published there as well.   Worm Development Dynamics Database (WDDD) The WDDD aims to acquire quantitative data...Koji, et al. "WDDD: worm developmental dynamics database." Nucleic acids research 41.D1 (2012): D732-D737...

  33. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    ...you can extract practically every single reliable base pair from your results. This option is not as fast...sequence databases from the dropdown menu. Typically, we use the default nucleotide database “Nucleotide...plasmids with us or other entries in NCBI’s numerous databases. As our repository has grown over the years (we... explanation of the different BLAST programs, databases, and elements of the BLAST search pages. At Addgene...known reference sequence or to a BLAST sequence database. If you know the expected nucleotide sequence,...identity between your sequence and the reference/database sequence is ≥ 95%. [Our QC process would be trouble-free...blastx checks all six possible frames against the database. Another benefit is that a frameshift mutation...

  34. Antibodies 101: Flow Compensation

    Type
    Blog Post
    Published
    May 16, 2024, 1:15 p.m.
    ...populations.   Figure 2: Compensation example based on experimental sample. Flow plots showing a sample...

  35. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...in-frame to the protein of interest for plasmid-based expression systems. Degron tags are one of many ...

  36. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...neutralized by pre-existing antibodies against AAV9-based vectors (and vice versa), enabling repeat dosing...

  37. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ...Voltage Sensitive Domain (VSD) GEVIs Simply put, VSD-based sensors consist of a VSD fused to a single fluorescent...

  38. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ...clozapine-N-oxide, clozapine, and compound 21 in DREADD-based chemogenetics in mice. Scientific Reports 9: https...

  39. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...Antibodies Plasmids 101: Peptide Tagging Plasmid-based Recombinant Antibody Resources on Addgene.org  ...
Showing: 441 - 480 of 576 results