We narrowed to 583 results for: BASE;

... Fig. 3: Development of a cleavage-free dCas9-based gene editor using microbial SSAPs. Image from Wang... system has a much lower error rate. For a one kilobase insertion, the researchers reported an editing...AAV system. dCas9-SSAP will make the editing of kilobase-scaled sequences more efficient and less risky...

...that the antibody is guaranteed to work. Antibody-based assays are complex and antibodies may perform differently...

...is one of the most complex subjects in antibody-based protocols - but thankfully this is an Antibody 101...

... to identify each protein of interest, this gel-based method identifies proteins solely by weight and ...

...about the advantages of using recombinant plasmid-based antibodies Resources on Addgene.org Discover Addgene...

...statement. Make certain to choose your recommenders based on your relationships to the person as well as their...resources, we created STEAMid, for a nationwide database of  STEAM internships for high school, college...

..., SRIRACCHA and GEMCherry2. We will cover single base-pair editing CRISPR reporters in the near future...

...specificity. This can be especially important for base editing techniques, where Cas9 must be directed ...

...screens of immune cell activity. However, a CRISPR-based tool for in vivo functional analysis of immune cells...

...wanted, the team identified Archon1, a novel opsin-based fluorescent voltage indicator. Archon1 can embed...

...NIR photosensitizer that allows for precise light-based control of protein inactivation/cell ablation while...

...weeks of exposure to pH 5.1 (Potter et al., 2014). Based on our results and the results reported elsewhere...

...Blast Most of Addgene’s lentiviral inventory is based on CRISPR tools, though we do provide a few non-...

.... Such disparities might suggest underlying sex-based influences on physiologic or pathologic processes...

...reliable sequence data back for the first 50-100 base pairs of your sequencing read. You can expect to...mammalian selection markers. You don’t need a fancy database to organize your own plasmid collection, although...help of DNA-editing software which integrates our database of over 2000 primers. Two 96-well PCR plates are...Finally, you will want to have a well-organized database of expected sequences, either for the inserts ...Get Plasmid Backbone Information from Our Vector Database  ...

...This article is based on an interview with Novartis researcher, Carrie Bhang. The ClonTracer Library,...

... CRISPR guides that flank regions of hundreds of base pairs or more can simultaneously introduce a pair...

...marker help synthesize? Counterselection? (growth-based positive selection for the loss of the marker gene...

...provide recombinant antibodies, another plasmid-based product that, with Addgene’s support, could fill...

...M., Shalik, I. K., & Yudkin, D. V. (2023). AAV- based vector improvements unrelated to capsid protein ...

...and optimization of a real-time quantitative PCR-based method for the titration of AAV-2 vector stocks....

...beyond generating frameshift mutations or single base pair edits, these enzymes are worth taking a look...Cas3 DNA digestion can proceed up to multiple kilobases, but only in one direction. Cas3 is thus not helpful...

...Gγ). G proteins are divided into four subtypes, based on their Gα subunit: Gαs, Gαi/o, Gαq/11, and Gα12...

...with BioRender.com. To make channelrhodopsin-based optogenetics minimally-invasive, Frances Arnold’...

...and RNAProtect® from Qiagen, are ammonium sulfate-based solutions that work by inhibiting RNase activity...

...distinguished. The UCLA Library offers some Powerpoint based templates with different column numbers for a good...

..., Portugal. She leads the Glycoimmunology group based at the Research Unit on Applied Molecular Biosciences...

...difficult for individual labs to complete. The nanobody based RANbody platform from the Sanes Lab overcomes this...

...got a full time position with a Washington D.C.-based communications consulting firm, Palladian Partners...

... at present.   Another issue surrounding CRISPR-based therapy is the level of testing needed to define...

...having effects on various biological disciplines, the base technology itself is constantly improving. Cas9 ...

...References 1.Tang, Jonathan C.Y., et al. “A nanobody-based system using fluorescent proteins as scaffolds for...

...recent models of C. elegans molecular biology are based on the standard worm genome. While C. elegans are...

...search engine and sign up for Google Scholar alerts. Based on keywords that you provide, Google Scholar will...

...used to restrict expression to a single cell type based on the overlap in cell types both promoters expressed...Institute of Molecular and Clinical Ophthalmology Basel recently took a different approach to enhance specificity...targeting the same cell class in humans (R=0.67).  A database of the microscopy images of all AAVs that reproducibly...

...The Sternberg lab in 2016 created cGAL, a GAL-4 based bipartite expression system that can regulate gene... is curated and added to other databases including wormbase and flybase. microPublication is run and maintained...pages and curated databases for scientists including Wormbook, Wormatlas and Wormbase. And scientists have...institute developed a 3’UTR database specifically for C. elegans. This database provides detailed information...coding mRNAs, and annotations extracted from other databases (Mangone et al., 2008).  OpenWorm OpenWorm is ...published there as well.   Worm Development Dynamics Database (WDDD) The WDDD aims to acquire quantitative data...Koji, et al. "WDDD: worm developmental dynamics database." Nucleic acids research 41.D1 (2012): D732-D737...

...Fluorescence fLuctuation INcrease by Contact (FLINC)-based biosensors measure fluctuations of TagRFP-T fluorescence...

...side of the optogenetics coin is materials science-based. Light is delivered to your opsin through a small...

...a final list of questions for the actual event, based on these interviews. The idea is to ask questions...

...you can extract practically every single reliable base pair from your results. This option is not as fast...sequence databases from the dropdown menu. Typically, we use the default nucleotide database “Nucleotide...plasmids with us or other entries in NCBI’s numerous databases. As our repository has grown over the years (we... explanation of the different BLAST programs, databases, and elements of the BLAST search pages. At Addgene...known reference sequence or to a BLAST sequence database. If you know the expected nucleotide sequence,...identity between your sequence and the reference/database sequence is ≥ 95%. [Our QC process would be trouble-free...blastx checks all six possible frames against the database. Another benefit is that a frameshift mutation...