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  2. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...original, though its maturation is even faster. Published just a few days later, Mohan Balasubramanian and...

  5. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...synthetic human scFV library and characterized them by ELISA, homogeneous time resolved fluorescence, and suspension...

  6. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ..., M., Farouni, R., Lagler, S., Kalamakis, G., Lischetti, U., Jabaudon, D., & Platt, R. J. (2023). Transcriptional...

  7. Common Injection Routes in Mice

    Type
    Blog Post
    Published
    Jan. 22, 2025, 9:42 p.m.
    ... for you — and the mice. While not an exhaustive list, these can include what’s being injected, the type...

  11. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ...could make a model of Cas9 based on the structure published by Martin Jinek’s group. We had already made an...

  13. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ...handy guide from OpenWetWare for a more extensive listing of common E. coli strains. Increase the volume ...

  19. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    ...alignment tools available. Try these resources for lists of alternatives to BLAST: ExPASy - http://www.expasy.org...

  20. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...pH changes (e.g. pHluorin, pHTomato). Keep this list handy as you plan your next experiment or to hand...

  24. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...adapted from the 1979 Patterson Protocol. When published, the Patterson Protocol was shown to purify DNA...

  25. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ... of parameters influencing droplet rain in the Listeria monocytogenes prfA assay-reduction of ambiguous...

  27. Plasmids 101: Colony PCR

    Type
    Blog Post
    Published
    May 12, 2016, 2:30 p.m.
    ...restriction enzyme digest; however colony PCR can accomplish the same thing in less time and for less money...

  34. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...mTurquoise2, mNeonGreen, and mScarlet-I for labeling a list of specific structures within cells. For example...

  35. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    Published
    March 31, 2020, 1:15 p.m.
    ...2012) Bacterial plasmid partition machinery: a minimalist approach to survival. Current Opinion in Structural...

  36. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    Published
    Jan. 23, 2020, 2:45 p.m.
    ...these libraries as ingredients to a recipe. It’s unrealistic to plant, grow, and harvest grains every time...

  39. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ...consider when designing a full gene. A non-exhaustive list includes: Codon abundance relative to cognate tRNA...

  41. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ... yourself for potential gRNA sites, or you can utilise several online tools that do it for you. One such...

  42. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...appear to be very effective probes for finely visualising sub-cellular structures or low-abundance proteins...

  44. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...Creating a bioinformatics workflow in Nextflow Establishing a complete pipeline however, required more than...

  49. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    Published
    June 27, 2024, 1:15 p.m.
    ...system, as is commonly used in western blotting or ELISAs.  A popular method for indirect labeling employs...
Showing: 551 - 600 of 611 results