We narrowed to 619 results for: LIS

... of pushing them in one by one. It seems very simplistic and it isn’t electronic but it is one of the ...

...Cas9 targeting of splice sites. Nat Biotechnol. Published online November 5, 2018. doi:10.1038/nbt.4283 ...

...original, though its maturation is even faster. Published just a few days later, Mohan Balasubramanian and...

...Any tags, selectable markers, and fusion proteins listed for each plasmid  If the plasmids pass all inspection...

...Originally published Aug 3, 2017 and last updated Apr 6, 2021 by Will Arnold. Introducing NGS in 2017...

...take to get an answer, consider asking to avoid unrealistic expectations. And finally, provide feedback...

...were held during the weekend. I attended multiple CLIs and benefited greatly by improving my skills and...

...makerspaces around the world. Hackerspaces.org maintains a list of active spaces near you.   Learning about C. ...

...could make a model of Cas9 based on the structure published by Martin Jinek’s group. We had already made an...

...protein, we have used Western blots, dot blots, ELISAs, and enzyme assays to select both over- and under-expressing...

... stocks that are available to everyone. Flybase lists what stocks are available at Bloomington, but you...

...  Equally as important is to be able to actively listen - this can be hard - but it is the best way to ...

...Tompkins, G. J., & McCawley, P. (1977). The establishment of two cell lines from the insectspodoptera ...

...range of topics. He embraces open science, self-publishing directly on his website (and often posting updates...

..., M., Farouni, R., Lagler, S., Kalamakis, G., Lischetti, U., Jabaudon, D., & Platt, R. J. (2023). Transcriptional...

... for you — and the mice. While not an exhaustive list, these can include what’s being injected, the type...

...synthetic human scFV library and characterized them by ELISA, homogeneous time resolved fluorescence, and suspension...

...gene of interest will direct the investigator to a list of all available Addgene plasmids he or she can ...

...handy guide from OpenWetWare for a more extensive listing of common E. coli strains. Increase the volume ...

...dimeric FP could ruin your day (and experiment) are listed below. Solutions to avoid these all too common ...

...negligible level. One major reason we chose to publish the concept and likely capabilities of RNA-guided...

...purification; the whole cloning process is so simplistic once you have your parts. That was the motivation...

...alignment tools available. Try these resources for lists of alternatives to BLAST: ExPASy - http://www.expasy.org...

...pH changes (e.g. pHluorin, pHTomato). Keep this list handy as you plan your next experiment or to hand...

... selected and ranked based on the previously established sgRNA rules and  potential on- and off-target...

...genome engineering in bacteria, alongside more established tools such as Cas9-recombineering, CRISPRa/i,...

...embed_player" overrideable=False, type='scriptV4', hide_playlist=True, viral_sharing=False, embed_button=False...

...answered the above questions, you can create your short list of options and then pick whichever is most accessible...

... toxic to dividing cells. The other antibiotics listed below affect continuous cellular processes (e.g...

...adapted from the 1979 Patterson Protocol. When published, the Patterson Protocol was shown to purify DNA...

... of parameters influencing droplet rain in the Listeria monocytogenes prfA assay-reduction of ambiguous...

...restriction enzyme digest; however colony PCR can accomplish the same thing in less time and for less money...

... the viral mRNAs and minigenome. This can be accomplished in multiple ways: transfecting with an additional...

...to yeast transformation protocols also helped establish the yeast two-hybrid (Y2H) system as a robust ...

...grant situation. So, young researchers need to publish good publications in a short period of time and...

...within neuronal networks in living animals. To accomplish such cell specific manipulations in vivo, FLEx...

...applications of CRISPR. Since this post was originally published, we've also seen the new development of Cas13b...

...consider when designing a full gene. A non-exhaustive list includes: Codon abundance relative to cognate tRNA...

...safe-targeting guides as a control will result in a smaller list of true positive hits than when using non-targeting...

...appear to be very effective probes for finely visualising sub-cellular structures or low-abundance proteins...

... a graphical representation of the results and a list of the analyzed samples (see below).   If the sample...

...Central PMCID: PMC6333924. Howerton, Alexis R., Alison V. Roland, and Tracy L. Bale. "Dorsal raphe neuroinflammation...

...mTurquoise2, mNeonGreen, and mScarlet-I for labeling a list of specific structures within cells. For example...

...work — in their system. The data is curated and published with a DOI, allowing others to reference and cite...

...have been used successfully in experiments and published in peer reviewed journals. If you need to generate...

...Creating a bioinformatics workflow in Nextflow Establishing a complete pipeline however, required more than...

...it isn’t a good fit for all experiments. And the list of antibiotic options doesn’t end there. Make sure...

...2012) Bacterial plasmid partition machinery: a minimalist approach to survival. Current Opinion in Structural...

...these libraries as ingredients to a recipe. It’s unrealistic to plant, grow, and harvest grains every time...

... yourself for potential gRNA sites, or you can utilise several online tools that do it for you. One such...